Rabbit Recombinant Monoclonal IRF3 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 2 publications.
Images
![Western blot - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (AB201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--western-blot-img8910.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (AB201809)")
![Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (AB201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--immunocytochemistry-immunofluorescence-img14557.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (AB201809)")
![Flow Cytometry (Intracellular) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (AB201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--flow-cytometry-intracellular-img12611.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (AB201809)")
![Western blot - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (AB201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--western-blot-img54096.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (AB201809)")
![Western blot - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (AB201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--western-blot-img54285.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (AB201809)")
Publications
Filter by
- Journal of virology 86:4538-472012C7L family of poxvirus host range genes inhibits antiviral activities induced by type I interferons and interferon regulatory factor 1.
- Nature cell biology 13:254-622011Klotho suppresses RIG-I-mediated senescence-associated inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Liu et. al.PubMed 21336305
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested | Expected |
| Mouse | Not recommended | Expected | Expected | Expected | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
10 products for Alternative Product
5 products for Alternative Version
Target data
Function
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed:22394562, PubMed:25636800, PubMed:27302953). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:11846977, PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:32972995). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed:16846591, PubMed:16979567, PubMed:20049431). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed:22394562, PubMed:25636800, PubMed:27302953). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed:16154084, PubMed:27302953, PubMed:33440148). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed:16846591). In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis (PubMed:25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
Alternative names
Interferon regulatory factor 3, IRF-3, IRF3
Recommended products
Rabbit Recombinant Monoclonal IRF3 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 2 publications.
Alternative names
Interferon regulatory factor 3, IRF-3, IRF3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR2418Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab201809 is the carrier-free version of Anti-IRF3 antibody [EPR2418Y] ab68481.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IRF3 also known as Interferon Regulatory Factor 3 acts as an important transcription factor in the immune response. It has a molecular weight of approximately 47 kDa. The IRF3 protein is mainly expressed in the cytoplasm and nucleus of various cell types including immune cells such as macrophages and dendritic cells. The protein becomes activated through phosphorylation a process frequently identified in its phosphorylated form phospo-IRF3 or p-IRF3 which facilitates its role in immune function.
- Biological function summary
IRF3 participates in the regulation of type I interferon (IFN) response a fundamental antiviral defense mechanism. IRF3 when phosphorylated forms a complex with CBP/p300 which then translocates to the nucleus to drive the expression of IFN-stimulated genes. This action strengthens the innate immune response and boosts the body's ability to counteract viral infections. Its activity and regulation are significant for maintaining a balanced immune response without excessive inflammation.
- Pathways
IRF3 is involved in the Toll-like receptor (TLR) and RIG-I-like receptor (RLR) signaling pathways both essential in pathogen recognition and response. Within these pathways IRF3 interacts with proteins such as MAVS and TBK1 to propagate immune signaling. The activation of IRF3 in these pathways results in the production of type I interferons and other cytokines orchestrating an effective antiviral response. These interactions highlight the protein's central role in mediating immune signaling cascades.
- Associated diseases and disorders
IRF3's malfunction or deregulation can contribute to autoimmune diseases and antiviral deficiencies. Conditions such as systemic lupus erythematosus (SLE) and chronic hepatitis B infection are linked to IRF3 activity. During autoimmune responses or viral persistence the aberrant activation of IRF3 can lead to inappropriate immune responses. The connection with proteins like STAT1 in these conditions highlights the complex network IRF3 engages in facilitating its impact on disease progression and immune dysregulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Western blot - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--western-blot-img8910.jpg "Western blot - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)")
Western blot - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)
This data was developed using the same antibody clone in a different buffer formulation (Anti-IRF3 antibody [EPR2418Y] ab68481).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-IRF3 antibody [EPR2418Y] ab68481 observed at 50 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-IRF3 antibody [EPR2418Y] ab68481 was shown to react with IRF3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human IRF3 knockout HeLa cell line ab255345 (knockout cell lysate Human IRF3 knockout HeLa cell lysate ab263784) was used. Wild-type and IRF3 knockout samples were subjected to SDS-PAGE. Anti-IRF3 antibody [EPR2418Y] ab68481 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-IRF3 antibody [EPR2418Y] (Anti-IRF3 antibody [EPR2418Y] ab68481) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 20 µg
Lane 2: MCF7 cell lysate at 20 µg
Lane 3: Wild-type HeLa cell lysate at 20 µg
Lane 4: IRF3 knockout HeLa cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 37 kDa, 51 kDa
Lanes 1 - 4: Merged signal (red and green). Green - Anti-IRF3 antibody [EPR2418Y] ab68481 observed at 50 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-IRF3 antibody [EPR2418Y] ab68481 was shown to react with IRF3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human IRF3 knockout HeLa cell line ab255345 (knockout cell lysate Human IRF3 knockout HeLa cell lysate ab263784) was used. Wild-type and IRF3 knockout samples were subjected to SDS-PAGE. Anti-IRF3 antibody [EPR2418Y] ab68481 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
![Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--immunocytochemistry-immunofluorescence-img14557.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)")
Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling IRF3 with Anti-IRF3 antibody [EPR2418Y] ab68481 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/400 dilution was used as the secondary antibody (green). The confocal image shows cytoplasmic on HeLa cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. Anti-IRF3 antibody [EPR2418Y] ab68481 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF3 antibody [EPR2418Y] ab68481).
![Flow Cytometry (Intracellular) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--flow-cytometry-intracellular-img12611.jpg "Flow Cytometry (Intracellular) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)")
Flow Cytometry (Intracellular) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)
Overlay histogram showing HAP1 wildtype (green line) and HAP1-IRF3 knockout cells (red line) stained with Anti-IRF3 antibody [EPR2418Y] ab68481. The cells were fixed with 80% methanol (5 min) (left pannel) or 4% formaldehyde (10 min) (right pannel), and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (Anti-IRF3 antibody [EPR2418Y] ab68481, 0.1μg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) presorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution for 30 min at 22°C. A rabbit IgG isotype control antibody (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-IRF3 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity). Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
Note: We recommend fixing cells using MeOH instead of PFA to get optimal results.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF3 antibody [EPR2418Y] ab68481).
![Western blot - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--western-blot-img54096.jpg "Western blot - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)")
Western blot - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)
This data was developed using the same antibody clone in a different buffer formulation (Anti-IRF3 antibody [EPR2418Y] ab68481).
Lanes 1 - 2: Merged signal (red and green). Green - Anti-IRF3 antibody [EPR2418Y] ab68481 observed at 50 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
Anti-IRF3 antibody [EPR2418Y] ab68481 was shown to react with IRF3 in wild-type A549 cells in western blot with loss of signal observed in IRF3 knockout cell line Human IRF3 knockout A549 cell line ab267098 (IRF3 knockout cell lysate Human IRF3 knockout A549 cell lysate ab256954). Wild-type and IRF3 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with Anti-IRF3 antibody [EPR2418Y] ab68481 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-IRF3 antibody [EPR2418Y] (Anti-IRF3 antibody [EPR2418Y] ab68481) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: IRF3 knockout A549 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa
![Western blot - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--western-blot-img54285.jpg "Western blot - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)")
Western blot - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)
This data was developed using the same antibody clone in a different buffer formulation (Anti-IRF3 antibody [EPR2418Y] ab68481).
Lanes 1 - 2: Merged signal (red and green). Green - Anti-IRF3 antibody [EPR2418Y] ab68481 observed at 50 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
Anti-IRF3 antibody [EPR2418Y] ab68481 was shown to react with IRF3 in wild-type A549 cells in western blot with loss of signal observed in IRF3 knockout cell line Human IRF3 knockout A549 cell line ab267097 (IRF3 knockout cell lysate Human IRF3 knockout A549 cell lysate ab256953). Wild-type and IRF3 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with Anti-IRF3 antibody [EPR2418Y] ab68481 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-IRF3 antibody [EPR2418Y] (Anti-IRF3 antibody [EPR2418Y] ab68481) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: IRF3 knockout A549 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa
![Flow Cytometry (Intracellular) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--flow-cytometry-intracellular-img26514.jpg "Flow Cytometry (Intracellular) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)")
Flow Cytometry (Intracellular) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)
Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed U937 (Human histiocytic lymphoma cells) cells labeling IRF3 with Anti-IRF3 antibody [EPR2418Y] ab68481 at 1/160 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF3 antibody [EPR2418Y] ab68481).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128451.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)
Immunohistochemical analysis of paraffin-embedded Mouse spleen labeling IRF3 with ab68481at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF3 antibody [EPR2418Y] ab68481).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129253.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)
Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix labeling IRF3 with ab68481at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF3 antibody [EPR2418Y] ab68481).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57193.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)
Immunohistochemical analysis of paraffin-embedded Human tonsil labeling IRF3 with ab68481at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF3 antibody [EPR2418Y] ab68481).
![Flow Cytometry (Intracellular) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--flow-cytometry-intracellular-img26514.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128451.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129253.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-epr2418y-bsa-and-azide-free-ab201809--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57193.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [EPR2418Y] - BSA and Azide free (ab201809)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com