Mouse Monoclonal IRF3 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IRF3 aa 1-150.
Images
![Flow Cytometry - Anti-IRF3 antibody [IRF35I218] (AB50772), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-irf35i218-ab50772--flow-cytometry-img27489.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-IRF3 antibody [IRF35I218] (AB50772)")
![Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-irf35i218-ab50772--western-blot-img49067.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772)")
![Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [IRF35I218] (AB50772), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-irf35i218-ab50772--immunocytochemistry-immunofluorescence-img54240.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [IRF35I218] (AB50772)")
![Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-irf35i218-ab50772--western-blot-img44435.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772)")
![Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-irf35i218-ab50772--western-blot-img435768.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772)")
Publications
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- mBio 14:e00332232023Human OTUD6B positively regulates type I IFN antiviral innate immune responses by deubiquitinating and stabilizing IRF3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Wang,Hui Zheng,Chunsheng Dong,Sidong XiongPubMed 37650650
- Nature 604:557-5622022Activation of STING by targeting a pocket in the transmembrane domain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDefen Lu,Guijun Shang,Jie Li,Yong Lu,Xiao-Chen Bai,Xuewu ZhangPubMed 35388221
- PLoS pathogens 16:e10090282020Vesicular trafficking permits evasion of cGAS/STING surveillance during initial human papillomavirus infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrittany L Uhlorn,Robert Jackson,Shuaizhi Li,Shauna M Bratton,Koenraad Van Doorslaer,Samuel K CamposPubMed 33253291
- Life science alliance 3:2020Attenuation of cGAS/STING activity during mitosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrittany L Uhlorn et. al.PubMed 32661021
- International journal of oncology 52:1623-16322018Interferon regulatory factor 3 mediates Poly(I:C)-induced innate immune response and apoptosis in non‑small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Yi et. al.PubMed 29512705
- Molecular therapy. Methods & clinical development 6:194-2062017Global Screening of Antiviral Genes that Suppress Baculovirus Transgene Expression in Mammalian Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChia-Hung Wang et. al.PubMed 28831401
- PLoS pathogens 12:e10059672016Histone H2B-IFI16 Recognition of Nuclear Herpesviral Genome Induces Cytoplasmic Interferon-β Responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJawed Iqbal et. al.PubMed 27764250
- Journal of virology 88:1582-902013Direct, interferon-independent activation of the CXCL10 promoter by NF-κB and interferon regulatory factor 3 during hepatitis C virus infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica Brownell,Jacob Bruckner,Jessica Wagoner,Emmanuel Thomas,Yueh-Ming Loo,Michael Gale,T Jake Liang,Stephen J PolyakPubMed 24257594
- The Journal of biological chemistry 286:10225-322011Morphine inhibits murine dendritic cell IL-23 production by modulating Toll-like receptor 2 and Nod2 signaling.
- Journal of immunology (Baltimore, Md. : 1950) 183:4422-312009TLR3 ligand polyinosinic:polycytidylic acid induces IL-17A and IL-21 synthesis in human Th cells.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 1.16% Sodium chloride, 1% BSA, 0.316% Tris HCl- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human IRF3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q14653
Reactivity data
Select an application| Flow Cyt | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed:22394562, PubMed:25636800, PubMed:27302953). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:11846977, PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:32972995). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed:16846591, PubMed:16979567, PubMed:20049431). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed:22394562, PubMed:25636800, PubMed:27302953). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed:16154084, PubMed:27302953, PubMed:33440148). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed:16846591). In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis (PubMed:25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
Alternative names
Interferon regulatory factor 3, IRF-3, IRF3
Recommended products
Mouse Monoclonal IRF3 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IRF3 aa 1-150.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 1.16% Sodium chloride, 1% BSA, 0.316% Tris HCl- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human IRF3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q14653
- Clone number
IRF35I218
- Purity
Tissue culture supernatant
- Concentration
- Purification notes
Concentrated and dialyzed culture medium (protein free medium) of hybridoma
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
![Flow Cytometry - Anti-IRF3 antibody [IRF35I218] (ab50772), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-irf35i218-ab50772--flow-cytometry-img27489.jpg "Flow Cytometry - Anti-IRF3 antibody [IRF35I218] (ab50772)")
Flow Cytometry - Anti-IRF3 antibody [IRF35I218] (ab50772)
Overlay histogram showing HeLa cells stained with ab50772 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab50772, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-irf35i218-ab50772--western-blot-img49067.jpg "Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772)")
Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772)
All lanes: Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772) at 1/50 dilution
All lanes: HT-1080 whole cell lysate at 25 µg
SecondaryAll lanes: Anti mouse IgG at 1/2500 dilution
Predicted band size: 47 kDa
Observed band size: 55 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [IRF35I218] (ab50772), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-irf35i218-ab50772--immunocytochemistry-immunofluorescence-img54240.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [IRF35I218] (ab50772)")
Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [IRF35I218] (ab50772)
Immunofluorescent staining of HeLa cells with a 1/10 dilution of ab50772 as primary antibody and AlexaFluor®488 conjugated Goat Anti-mouse IgG diluted 1/200 as the secondary antibody
![Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-irf35i218-ab50772--western-blot-img44435.jpg "Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772)")
Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772)
IRF3 antibody [IRF35I218] (ab50772) at 1/100 dilution + Ripa lysate of Hela cells at 500 µg
Secondary
Anti-mouse IgG at 1/1000 dilutionAll lanes: Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772) at 1/100 dilution
All lanes: Ripa lysate of Hela cells at 500 µg
SecondaryAll lanes: Anti-mouse IgG at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 55 kDa
![Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772), expandable thumbnail](https://content.abcam.com/products/images/irf3-antibody-irf35i218-ab50772--western-blot-img435768.jpg "Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772)")
This image is courtesy of an anonymous customer reviewWestern blot - Anti-IRF3 antibody [IRF35I218] (ab50772)
All lanes: Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772) at 1/2000 dilution
All lanes: Human THP1 lysate at 25 µg
SecondaryAll lanes: HRP Goat Anti Mouse Secondary Antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1min
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