AB50772

Anti-IRF3 antibody [IRF35I218]

Name: Anti-IRF3 antibody [IRF35I218] (ab50772) | Abcam
Brand: Abcam Limited
SKU: AB50772
Price: 510 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(11 Publications)

Mouse Monoclonal IRF3 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IRF3 aa 1-150.

View Alternative Names

Interferon regulatory factor 3, IRF-3, IRF3

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [IRF35I218] (AB50772)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [IRF35I218] (AB50772)

Immunofluorescent staining of HeLa cells with a 1/10 dilution of ab50772 as primary antibody and AlexaFluor®488 conjugated Goat Anti-mouse IgG diluted 1/200 as the secondary antibody

Flow Cytometry - Anti-IRF3 antibody [IRF35I218] (AB50772)

Flow Cyt

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Flow Cytometry - Anti-IRF3 antibody [IRF35I218] (AB50772)

Overlay histogram showing HeLa cells stained with ab50772 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab50772, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

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Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772)

IRF3 antibody [IRF35I218] (ab50772) at 1/100 dilution + Ripa lysate of Hela cells at 500 µg Secondary Anti-mouse IgG at 1/1000 dilution

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Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772) at 1/100 dilution

All lanes:

Ripa lysate of Hela cells at 500 µg

Secondary

All lanes:

Anti-mouse IgG at 1/1000 dilution

Predicted band size: 47 kDa

Observed band size: 55 kDa

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Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772)

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Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772) at 1/50 dilution

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HT-1080 whole cell lysate at 25 µg

Secondary

All lanes:

Anti mouse IgG at 1/2500 dilution

Predicted band size: 47 kDa

Observed band size: 55 kDa

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AbReview65364****

Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772)

All lanes:

Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772) at 1/2000 dilution

All lanes:

Human THP1 lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti Mouse Secondary Antibody at 1/10000 dilution

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Exposure time: 1min

This image is courtesy of an anonymous Abreview

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

IRF35I218

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human IRF3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q14653

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/100", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification notes

Concentrated and dialyzed culture medium (protein free medium) of hybridoma

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: 1.16% Sodium chloride, 1% BSA, 0.316% Tris HCl

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRF3 also known as Interferon Regulatory Factor 3 acts as an important transcription factor in the immune response. It has a molecular weight of approximately 47 kDa. The IRF3 protein is mainly expressed in the cytoplasm and nucleus of various cell types including immune cells such as macrophages and dendritic cells. The protein becomes activated through phosphorylation a process frequently identified in its phosphorylated form phospo-IRF3 or p-IRF3 which facilitates its role in immune function.

Biological function summary

IRF3 participates in the regulation of type I interferon (IFN) response a fundamental antiviral defense mechanism. IRF3 when phosphorylated forms a complex with CBP/p300 which then translocates to the nucleus to drive the expression of IFN-stimulated genes. This action strengthens the innate immune response and boosts the body's ability to counteract viral infections. Its activity and regulation are significant for maintaining a balanced immune response without excessive inflammation.

Pathways

IRF3 is involved in the Toll-like receptor (TLR) and RIG-I-like receptor (RLR) signaling pathways both essential in pathogen recognition and response. Within these pathways IRF3 interacts with proteins such as MAVS and TBK1 to propagate immune signaling. The activation of IRF3 in these pathways results in the production of type I interferons and other cytokines orchestrating an effective antiviral response. These interactions highlight the protein's central role in mediating immune signaling cascades.

IRF3's malfunction or deregulation can contribute to autoimmune diseases and antiviral deficiencies. Conditions such as systemic lupus erythematosus (SLE) and chronic hepatitis B infection are linked to IRF3 activity. During autoimmune responses or viral persistence the aberrant activation of IRF3 can lead to inappropriate immune responses. The connection with proteins like STAT1 in these conditions highlights the complex network IRF3 engages in facilitating its impact on disease progression and immune dysregulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed : 22394562, PubMed : 24049179, PubMed : 25636800, PubMed : 27302953, PubMed : 31340999, PubMed : 36603579, PubMed : 8524823). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed : 11846977, PubMed : 16846591, PubMed : 16979567, PubMed : 20049431, PubMed : 32972995, PubMed : 36603579, PubMed : 8524823). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed : 16846591, PubMed : 16979567, PubMed : 20049431, PubMed : 36603579). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed : 22394562, PubMed : 25636800, PubMed : 27302953, PubMed : 36603579). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed : 16154084, PubMed : 27302953, PubMed : 33440148, PubMed : 36603579). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed : 16846591). In response to Sendai virus infection, is recruited by TOMM70 : HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70 : HSP90AA1 : IRF3 : BAX inducing apoptosis (PubMed : 25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed : 33440148).

See full target information IRF3

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

mBio 14:e0033223 PubMed37650650

2023

Human OTUD6B positively regulates type I IFN antiviral innate immune responses by deubiquitinating and stabilizing IRF3.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wang,Hui Zheng,Chunsheng Dong,Sidong Xiong

Nature 604:557-562 PubMed35388221

2022

Activation of STING by targeting a pocket in the transmembrane domain.

Applications

Unspecified application

Species

Unspecified reactive species

Defen Lu,Guijun Shang,Jie Li,Yong Lu,Xiao-Chen Bai,Xuewu Zhang

PLoS pathogens 16:e1009028 PubMed33253291

2020

Vesicular trafficking permits evasion of cGAS/STING surveillance during initial human papillomavirus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Brittany L Uhlorn,Robert Jackson,Shuaizhi Li,Shauna M Bratton,Koenraad Van Doorslaer,Samuel K Campos

Life science alliance 3: PubMed32661021

2020

Attenuation of cGAS/STING activity during mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Brittany L Uhlorn,Eduardo R Gamez,Shuaizhi Li,Samuel K Campos

International journal of oncology 52:1623-1632 PubMed29512705

2018

Interferon regulatory factor 3 mediates Poly(I:C)-induced innate immune response and apoptosis in non‑small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Yi,Dan Sun,Qian Han,Zhonghui Liu,Zeng Zeng,Yanping Wu,Xiaoyu Chai,Xinmin Liu

Molecular therapy. Methods & clinical development 6:194-206 PubMed28831401

2017

Global Screening of Antiviral Genes that Suppress Baculovirus Transgene Expression in Mammalian Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Hung Wang,Nenavath Gopal Naik,Lin-Li Liao,Sung-Chan Wei,Yu-Chan Chao

PLoS pathogens 12:e1005967 PubMed27764250

2016

Histone H2B-IFI16 Recognition of Nuclear Herpesviral Genome Induces Cytoplasmic Interferon-β Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Jawed Iqbal,Mairaj Ahmed Ansari,Binod Kumar,Dipanjan Dutta,Arunava Roy,Leela Chikoti,Gina Pisano,Sujoy Dutta,Shahrooz Vahedi,Mohanan Valiya Veettil,Bala Chandran

Journal of virology 88:1582-90 PubMed24257594

2013

Direct, interferon-independent activation of the CXCL10 promoter by NF-κB and interferon regulatory factor 3 during hepatitis C virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Brownell,Jacob Bruckner,Jessica Wagoner,Emmanuel Thomas,Yueh-Ming Loo,Michael Gale,T Jake Liang,Stephen J Polyak

The Journal of biological chemistry 286:10225-32 PubMed21245149

2011

Morphine inhibits murine dendritic cell IL-23 production by modulating Toll-like receptor 2 and Nod2 signaling.

Applications

ICC/IF

Species

Mouse

Jinghua Wang,Jing Ma,Rick Charboneau,Roderick Barke,Sabita Roy

Journal of immunology (Baltimore, Md. : 1950) 183:4422-31 PubMed19748983

2009

TLR3 ligand polyinosinic:polycytidylic acid induces IL-17A and IL-21 synthesis in human Th cells.

Applications

Species

Human

Christian K Holm,Charlotte C Petersen,Malene Hvid,Line Petersen,Søren R Paludan,Bent Deleuran,Marianne Hokland

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-IRF3 antibody [IRF35I218]

Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [IRF35I218] (AB50772)

Flow Cytometry - Anti-IRF3 antibody [IRF35I218] (AB50772)

Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772)

Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772)

Western blot - Anti-IRF3 antibody [IRF35I218] (AB50772)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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