Rabbit Recombinant Monoclonal IRF3 phospho S386 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 9 publications.
Images
![Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (AB182859), expandable thumbnail](https://content.abcam.com/products/images/irf3-phospho-s386-antibody-epr2346-bsa-and-azide-free-ab182859--dot-blot-img18189.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (AB182859)")
Publications
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- Animal models and experimental medicine 6:245-2542023Anti-apoptotic protein BCL-XL as a therapeutic vulnerability in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYumin Wei,Liping Zhang,Chao Wang,Zefeng Li,Mingjie Luo,Guomin Xie,Xingjiu Yang,Mengyuan Li,Shuyue Ren,Dongbing Zhao,Ran Gao,Jia-Nan GongPubMed 37271936
- Journal of immunology (Baltimore, Md. : 1950) 191:3264-762013Melanoma differentiation-associated gene 5 senses hepatitis B virus and activates innate immune signaling to suppress virus replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHsin-Lin Lu et. al.PubMed 23926323
- PloS one 8:e537172013RING finger protein 11 targets TBK1/IKKi kinases to inhibit antiviral signaling.Applications:WBReactive species:Unspecified reactive speciesSoratree Charoenthongtrakul et. al.PubMed 23308279
- The Journal of experimental medicine 209:1567-822012Heterozygous TBK1 mutations impair TLR3 immunity and underlie herpes simplex encephalitis of childhood.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelina Herman et. al.PubMed 22851595
- Proceedings of the National Academy of Sciences of 109:9378-832012Molecular basis of Tank-binding kinase 1 activation by transautophosphorylation.
- The Journal of biological chemistry 286:44211-72011Galectin-9 protein expression in endothelial cells is positively regulated by histone deacetylase 3.
- The Journal of biological chemistry 286:36592-6022011ABIN1 protein cooperates with TAX1BP1 and A20 proteins to inhibit antiviral signaling.
- Nature cell biology 13:254-622011Klotho suppresses RIG-I-mediated senescence-associated inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Liu et. al.PubMed 21336305
- Cell research 20:994-10112010Tom70 mediates activation of interferon regulatory factor 3 on mitochondria.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | |
|---|---|---|
| Human | Predicted | Expected |
| Synthetic peptide | Tested | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
12 products for Alternative Product
Target data
Function
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed:22394562, PubMed:25636800, PubMed:27302953). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:11846977, PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:32972995). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed:16846591, PubMed:16979567, PubMed:20049431). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed:22394562, PubMed:25636800, PubMed:27302953). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed:16154084, PubMed:27302953, PubMed:33440148). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed:16846591). In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis (PubMed:25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
Alternative names
Interferon regulatory factor 3, IRF-3, IRF3
Recommended products
Rabbit Recombinant Monoclonal IRF3 phospho S386 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 9 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR2346
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab182859 is the carrier-free version of Anti-IRF3 (phospho S386) antibody [EPR2346] ab76493.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IRF3 also known as Interferon Regulatory Factor 3 acts as an important transcription factor in the immune response. It has a molecular weight of approximately 47 kDa. The IRF3 protein is mainly expressed in the cytoplasm and nucleus of various cell types including immune cells such as macrophages and dendritic cells. The protein becomes activated through phosphorylation a process frequently identified in its phosphorylated form phospo-IRF3 or p-IRF3 which facilitates its role in immune function.
- Biological function summary
IRF3 participates in the regulation of type I interferon (IFN) response a fundamental antiviral defense mechanism. IRF3 when phosphorylated forms a complex with CBP/p300 which then translocates to the nucleus to drive the expression of IFN-stimulated genes. This action strengthens the innate immune response and boosts the body's ability to counteract viral infections. Its activity and regulation are significant for maintaining a balanced immune response without excessive inflammation.
- Pathways
IRF3 is involved in the Toll-like receptor (TLR) and RIG-I-like receptor (RLR) signaling pathways both essential in pathogen recognition and response. Within these pathways IRF3 interacts with proteins such as MAVS and TBK1 to propagate immune signaling. The activation of IRF3 in these pathways results in the production of type I interferons and other cytokines orchestrating an effective antiviral response. These interactions highlight the protein's central role in mediating immune signaling cascades.
- Associated diseases and disorders
IRF3's malfunction or deregulation can contribute to autoimmune diseases and antiviral deficiencies. Conditions such as systemic lupus erythematosus (SLE) and chronic hepatitis B infection are linked to IRF3 activity. During autoimmune responses or viral persistence the aberrant activation of IRF3 can lead to inappropriate immune responses. The connection with proteins like STAT1 in these conditions highlights the complex network IRF3 engages in facilitating its impact on disease progression and immune dysregulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859), expandable thumbnail](https://content.abcam.com/products/images/irf3-phospho-s386-antibody-epr2346-bsa-and-azide-free-ab182859--dot-blot-img18189.jpg "Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)")
Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)
This Dot Blot data was generated using the same anti-phospho IRF3 S386 antibody clone, EPR2346, in a different buffer formulation (cat# Anti-IRF3 (phospho S386) antibody [EPR2346] ab76493).
Dot blot analysis of IRF3 single phospho peptide pS386 (lane 1) and IRF3 non-phospho peptide (lane 2) with Anti-IRF3 (phospho S386) antibody [EPR2346] ab76493 at 1/1000. Blocking and diluting buffer was 5% NFDM/TBST. The secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP) ab97051 Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/100,000.
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