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AB182859

Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free

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(9 Publications)

Rabbit Recombinant Monoclonal IRF3 phospho S386 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 9 publications.

View Alternative Names

Interferon regulatory factor 3, IRF-3, IRF3

1 Images
Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (AB182859)
  • Dot

Unknown

Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (AB182859)

This Dot Blot data was generated using the same anti-phospho IRF3 S386 antibody clone, EPR2346, in a different buffer formulation (cat# ab76493).

Dot blot analysis of IRF3 single phospho peptide pS386 (lane 1) and IRF3 non-phospho peptide (lane 2) with ab76493 at 1/1000. Blocking and diluting buffer was 5% NFDM/TBST. The secondary antibody used was ab97051 Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/100,000.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2346

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab182859 is the carrier-free version of ab76493.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRF3 also known as Interferon Regulatory Factor 3 acts as an important transcription factor in the immune response. It has a molecular weight of approximately 47 kDa. The IRF3 protein is mainly expressed in the cytoplasm and nucleus of various cell types including immune cells such as macrophages and dendritic cells. The protein becomes activated through phosphorylation a process frequently identified in its phosphorylated form phospo-IRF3 or p-IRF3 which facilitates its role in immune function.
Biological function summary

IRF3 participates in the regulation of type I interferon (IFN) response a fundamental antiviral defense mechanism. IRF3 when phosphorylated forms a complex with CBP/p300 which then translocates to the nucleus to drive the expression of IFN-stimulated genes. This action strengthens the innate immune response and boosts the body's ability to counteract viral infections. Its activity and regulation are significant for maintaining a balanced immune response without excessive inflammation.

Pathways

IRF3 is involved in the Toll-like receptor (TLR) and RIG-I-like receptor (RLR) signaling pathways both essential in pathogen recognition and response. Within these pathways IRF3 interacts with proteins such as MAVS and TBK1 to propagate immune signaling. The activation of IRF3 in these pathways results in the production of type I interferons and other cytokines orchestrating an effective antiviral response. These interactions highlight the protein's central role in mediating immune signaling cascades.

IRF3's malfunction or deregulation can contribute to autoimmune diseases and antiviral deficiencies. Conditions such as systemic lupus erythematosus (SLE) and chronic hepatitis B infection are linked to IRF3 activity. During autoimmune responses or viral persistence the aberrant activation of IRF3 can lead to inappropriate immune responses. The connection with proteins like STAT1 in these conditions highlights the complex network IRF3 engages in facilitating its impact on disease progression and immune dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed : 22394562, PubMed : 24049179, PubMed : 25636800, PubMed : 27302953, PubMed : 31340999, PubMed : 36603579, PubMed : 8524823). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed : 11846977, PubMed : 16846591, PubMed : 16979567, PubMed : 20049431, PubMed : 32972995, PubMed : 36603579, PubMed : 8524823). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed : 16846591, PubMed : 16979567, PubMed : 20049431, PubMed : 36603579). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed : 22394562, PubMed : 25636800, PubMed : 27302953, PubMed : 36603579). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed : 16154084, PubMed : 27302953, PubMed : 33440148, PubMed : 36603579). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed : 16846591). In response to Sendai virus infection, is recruited by TOMM70 : HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70 : HSP90AA1 : IRF3 : BAX inducing apoptosis (PubMed : 25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed : 33440148).
See full target information IRF3 phospho S386
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Animal models and experimental medicine 6:245-254 PubMed37271936

2023

Anti-apoptotic protein BCL-XL as a therapeutic vulnerability in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yumin Wei,Liping Zhang,Chao Wang,Zefeng Li,Mingjie Luo,Guomin Xie,Xingjiu Yang,Mengyuan Li,Shuyue Ren,Dongbing Zhao,Ran Gao,Jia-Nan Gong

Journal of immunology (Baltimore, Md. : 1950) 191:3264-76 PubMed23926323

2013

Melanoma differentiation-associated gene 5 senses hepatitis B virus and activates innate immune signaling to suppress virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Hsin-Lin Lu,Fang Liao

PloS one 8:e53717 PubMed23308279

2013

RING finger protein 11 targets TBK1/IKKi kinases to inhibit antiviral signaling.

Applications

WB

Species

Unspecified reactive species

Soratree Charoenthongtrakul,Linlin Gao,Kislay Parvatiyar,David Lee,Edward W Harhaj

The Journal of experimental medicine 209:1567-82 PubMed22851595

2012

Heterozygous TBK1 mutations impair TLR3 immunity and underlie herpes simplex encephalitis of childhood.

Applications

Unspecified application

Species

Unspecified reactive species

Melina Herman,Michael Ciancanelli,Yi-Hung Ou,Lazaro Lorenzo,Maja Klaudel-Dreszler,Elodie Pauwels,Vanessa Sancho-Shimizu,Rebeca Pérez de Diego,Avinash Abhyankar,Elisabeth Israelsson,Yiqi Guo,Annabelle Cardon,Flore Rozenberg,Pierre Lebon,Marc Tardieu,Edyta Heropolitanska-Pliszka,Damien Chaussabel,Michael A White,Laurent Abel,Shen-Ying Zhang,Jean-Laurent Casanova

Proceedings of the National Academy of Sciences of 109:9378-83 PubMed22619329

2012

Molecular basis of Tank-binding kinase 1 activation by transautophosphorylation.

Applications

WB

Species

Unspecified reactive species

Xiaolei Ma,Elizabeth Helgason,Qui T Phung,Clifford L Quan,Rekha S Iyer,Michelle W Lee,Krista K Bowman,Melissa A Starovasnik,Erin C Dueber

The Journal of biological chemistry 286:44211-7 PubMed22027828

2011

Galectin-9 protein expression in endothelial cells is positively regulated by histone deacetylase 3.

Applications

WB

Species

Human

Saydul Alam,Hongling Li,Andriana Margariti,Daniel Martin,Anna Zampetaki,Ouassila Habi,Gillian Cockerill,Yanhua Hu,Qingbo Xu,Lingfang Zeng

The Journal of biological chemistry 286:36592-602 PubMed21885437

2011

ABIN1 protein cooperates with TAX1BP1 and A20 proteins to inhibit antiviral signaling.

Applications

WB

Species

Unspecified reactive species

Linlin Gao,Helen Coope,Susan Grant,Averil Ma,Steven C Ley,Edward W Harhaj

Nature cell biology 13:254-62 PubMed21336305

2011

Klotho suppresses RIG-I-mediated senescence-associated inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Liu,Su Wu,Hongwei Ren,Jun Gu

Cell research 20:994-1011 PubMed20628368

2010

Tom70 mediates activation of interferon regulatory factor 3 on mitochondria.

Applications

WB

Species

Human

Xin-Yi Liu,Bo Wei,He-Xin Shi,Yu-Fei Shan,Chen Wang
View all publications
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