Rabbit Polyclonal IRF3 phospho S396 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.
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- Cell death & disease 13:6402022Mitotic SENP3 activation couples with cGAS signaling in tumor cells to stimulate anti-tumor immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaolei Hu,Yalan Chen,Xinyu Yang,Yang Wang,Jianli He,Tianshi Wang,Qiuju Fan,Liufu Deng,Jun Tu,Hongsheng Tan,Jinke ChengPubMed 35869062
- bioRxiv : the preprint server for biology :2021Self-assembling short immunostimulatory duplex RNAs with broad spectrum antiviral activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLonglong Si,Haiqing Bai,Crystal Yuri Oh,Tian Zhang,Fan Hong,Amanda Jiang,Yongxin Ye,Tristan X Jordan,James Logue,Marisa McGrath,Chaitra Belgur,Atiq Nurani,Wuji Cao,Rachelle Prantil-Baun,Steven P Gygi,Rani K Powers,Matthew Frieman,Benjamin R tenOever,Donald E IngberPubMed 34845453
- Frontiers in veterinary science 7:5705932020Effects of Probiotics BaSC06 on Intestinal Digestion and Absorption, Antioxidant Capacity, Microbiota Composition, and Macrophage Polarization in Pigs for Fattening.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuefang Cao et. al.PubMed 33240950
- Nature microbiology 5:584-5982020N-methyladenosine modification enables viral RNA to escape recognition by RNA sensor RIG-I.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMijia Lu et. al.PubMed 32015498
- Nature communications 9:12432018MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeipei Zhou et. al.PubMed 29593341
- International journal of molecular medicine 40:1921-19312017Paralemmin-3 contributes to lipopolysaccharide-induced inflammatory response and is involved in lipopolysaccharide-Toll-like receptor-4 signaling in alveolar macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu-Xin Chen et. al.PubMed 29039447
- Inflammation 40:1983-19992017Downregulation of Paralemmin-3 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Rats by Regulating Inflammatory Response and Inhibiting Formation of TLR4/MyD88 and TLR4/TRIF Complexes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuxin Chen,Lu Tang,Jian Feng,Yi Wang,Zhihai Han,Jiguang MengPubMed 28801798
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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7 products for Alternative Product
Target data
Function
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed:22394562, PubMed:24049179, PubMed:25636800, PubMed:27302953, PubMed:31340999, PubMed:36603579, PubMed:8524823). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:11846977, PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:32972995, PubMed:36603579, PubMed:8524823). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:36603579). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed:22394562, PubMed:25636800, PubMed:27302953, PubMed:36603579). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed:16154084, PubMed:27302953, PubMed:33440148, PubMed:36603579). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed:16846591). In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis (PubMed:25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
Alternative names
Interferon regulatory factor 3, IRF-3, IRF3
Recommended products
Rabbit Polyclonal IRF3 phospho S396 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab138449 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IRF3 also known as Interferon Regulatory Factor 3 acts as an important transcription factor in the immune response. It has a molecular weight of approximately 47 kDa. The IRF3 protein is mainly expressed in the cytoplasm and nucleus of various cell types including immune cells such as macrophages and dendritic cells. The protein becomes activated through phosphorylation a process frequently identified in its phosphorylated form phospo-IRF3 or p-IRF3 which facilitates its role in immune function.
- Biological function summary
IRF3 participates in the regulation of type I interferon (IFN) response a fundamental antiviral defense mechanism. IRF3 when phosphorylated forms a complex with CBP/p300 which then translocates to the nucleus to drive the expression of IFN-stimulated genes. This action strengthens the innate immune response and boosts the body's ability to counteract viral infections. Its activity and regulation are significant for maintaining a balanced immune response without excessive inflammation.
- Pathways
IRF3 is involved in the Toll-like receptor (TLR) and RIG-I-like receptor (RLR) signaling pathways both essential in pathogen recognition and response. Within these pathways IRF3 interacts with proteins such as MAVS and TBK1 to propagate immune signaling. The activation of IRF3 in these pathways results in the production of type I interferons and other cytokines orchestrating an effective antiviral response. These interactions highlight the protein's central role in mediating immune signaling cascades.
- Associated diseases and disorders
IRF3's malfunction or deregulation can contribute to autoimmune diseases and antiviral deficiencies. Conditions such as systemic lupus erythematosus (SLE) and chronic hepatitis B infection are linked to IRF3 activity. During autoimmune responses or viral persistence the aberrant activation of IRF3 can lead to inappropriate immune responses. The connection with proteins like STAT1 in these conditions highlights the complex network IRF3 engages in facilitating its impact on disease progression and immune dysregulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-IRF3 (phospho S396) antibody (ab138449)
All lanes: Western blot - Anti-IRF3 (phospho S396) antibody (ab138449) at 1/500 dilution
All lanes: Jurkat cells treated with 200 ng/ml of EGF for 30 minutes.
Predicted band size: 43 kDa, 46 kDa, 47 kDa, 51 kDa, 54 kDa
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