Rabbit Recombinant Monoclonal IRF4 antibody. Carrier free. Suitable for ICC/IF, IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EP5699] - BSA and Azide free (AB240071), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-ep5699-bsa-and-azide-free-ab240071--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136723.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EP5699] - BSA and Azide free (AB240071)")
![Flow Cytometry (Intracellular) - Anti-IRF4 antibody [EP5699] - BSA and Azide free (AB240071), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-ep5699-bsa-and-azide-free-ab240071--flow-cytometry-intracellular-img127548.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IRF4 antibody [EP5699] - BSA and Azide free (AB240071)")
![Immunoprecipitation - Anti-IRF4 antibody [EP5699] - BSA and Azide free (AB240071), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-ep5699-bsa-and-azide-free-ab240071--immunoprecipitation-img176176.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IRF4 antibody [EP5699] - BSA and Azide free (AB240071)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EP5699] - BSA and Azide free (AB240071), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-ep5699-bsa-and-azide-free-ab240071--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98927.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EP5699] - BSA and Azide free (AB240071)")
![Immunocytochemistry/ Immunofluorescence - Anti-IRF4 antibody [EP5699] - BSA and Azide free (AB240071), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-ep5699-bsa-and-azide-free-ab240071--immunocytochemistry-immunofluorescence-img432459.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IRF4 antibody [EP5699] - BSA and Azide free (AB240071)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IHC-P | IP | WB | Flow Cyt (Intra) | ChIP | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ChIP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
3 products for Alternative Version
Target data
Function
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity).
Alternative names
MUM1, IRF4, Interferon regulatory factor 4, IRF-4, Lymphocyte-specific interferon regulatory factor, Multiple myeloma oncogene 1, NF-EM5, LSIRF
Recommended products
Rabbit Recombinant Monoclonal IRF4 antibody. Carrier free. Suitable for ICC/IF, IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP5699
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240071 is the carrier-free version of Anti-IRF4 antibody [EP5699] ab133590.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The IRF4 protein also known as Interferon Regulatory Factor 4 is a transcription factor encoded by the IRF4 gene. It weighs approximately 52 kDa. IRF4 is expressed mainly in lymphoid cells including B cells T cells and macrophages. It plays a mechanical role in regulating gene expression by binding to specific DNA sequences. Through these interactions IRF4 influences various cellular processes important for the immune response.
- Biological function summary
IRF4 helps in the activation and differentiation of immune cells. It forms complexes with other transcription factors like PU.1 enhancing its regulatory functions. IRF4 also contributes to the maturation of B and T lymphocytes impacting the adaptive immune system. It acts by modulating expression of cytokines and surface markers involved in immune response.
- Pathways
IRF4 functions within the immune response and cell differentiation pathways. It interacts with pathways like NF-kB and MAPK linking it to important immune regulatory processes. IRF4 associates with proteins such as c-Rel in the NF-kB pathway influencing gene expression related to immune activation and survival.
- Associated diseases and disorders
IRF4 plays a role in multiple myeloma and autoimmune disorders. Aberrant expression of IRF4 can lead to dysregulated immune cell proliferation as seen in multiple myeloma. It also connects with proteins like BLIMP-1 contributing to pathogenesis of autoimmune conditions. Understanding IRF4’s involvement provides insights into potential therapeutic targets for such diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EP5699] - BSA and Azide free (ab240071), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-ep5699-bsa-and-azide-free-ab240071--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136723.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EP5699] - BSA and Azide free (ab240071)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EP5699] - BSA and Azide free (ab240071)
Immunohistochemical analysis of paraffin embedded Human lymphoma tissue labelling IRF4, using Anti-IRF4 antibody [EP5699] ab133590 at a dilution of 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF4 antibody [EP5699] ab133590).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-IRF4 antibody [EP5699] - BSA and Azide free (ab240071), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-ep5699-bsa-and-azide-free-ab240071--flow-cytometry-intracellular-img127548.jpg "Flow Cytometry (Intracellular) - Anti-IRF4 antibody [EP5699] - BSA and Azide free (ab240071)")
Flow Cytometry (Intracellular) - Anti-IRF4 antibody [EP5699] - BSA and Azide free (ab240071)
Overlay histogram showing Ramos cells stained with Anti-IRF4 antibody [EP5699] ab133590 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-IRF4 antibody [EP5699] ab133590, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF4 antibody [EP5699] ab133590).
![Immunoprecipitation - Anti-IRF4 antibody [EP5699] - BSA and Azide free (ab240071), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-ep5699-bsa-and-azide-free-ab240071--immunoprecipitation-img176176.jpg "Immunoprecipitation - Anti-IRF4 antibody [EP5699] - BSA and Azide free (ab240071)")
Immunoprecipitation - Anti-IRF4 antibody [EP5699] - BSA and Azide free (ab240071)
This data was developed using Anti-IRF4 antibody [EP5699] ab133590, the same antibody clone in a different buffer formulation.
IRF4 was immunoprecipitated from 0.35 mg IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate 10 μg with Anti-IRF4 antibody [EP5699] ab133590 at 1/20 dilution (1μg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate 10 μg
Lane 2: abab133590 IP in IM-9 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IRF4 antibody [EP5699] ab133590 in IM-9 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Lower bands could be ubiquitin mediated protein degradation. (PMID:23658517)
All lanes: Immunoprecipitation - Anti-IRF4 antibody [EP5699] (Anti-IRF4 antibody [EP5699] ab133590)
Predicted band size: 52 kDa
Observed band size: 52 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EP5699] - BSA and Azide free (ab240071), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-ep5699-bsa-and-azide-free-ab240071--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98927.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EP5699] - BSA and Azide free (ab240071)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EP5699] - BSA and Azide free (ab240071)
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling IRF4, using Anti-IRF4 antibody [EP5699] ab133590 at a dilution of 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF4 antibody [EP5699] ab133590).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-IRF4 antibody [EP5699] - BSA and Azide free (ab240071), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-ep5699-bsa-and-azide-free-ab240071--immunocytochemistry-immunofluorescence-img432459.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IRF4 antibody [EP5699] - BSA and Azide free (ab240071)")
Immunocytochemistry/ Immunofluorescence - Anti-IRF4 antibody [EP5699] - BSA and Azide free (ab240071)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF4 antibody [EP5699] ab133590).
Immunocytochemistry/ Immunofluorescence analysis of IM-9 (human multiple myeloma B lymphoblast) labeling IRF4 with Anti-IRF4 antibody [EP5699] ab133590 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. DAPI was used as nuclear counterstain. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 was used as the counterstain antibody.
Confocal image showing mainly nuclear staining in IM-9 cell line.
Negative control: 293T (PMID: 23804715)
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
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