Rabbit Monoclonal IRF4 antibody. Suitable for WB, IP and reacts with Mouse samples.
Images
![Immunoprecipitation - Anti-IRF4 antibody [EPR26379-107] (AB300459), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-epr26379-107-ab300459--immunoprecipitation-img400849.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IRF4 antibody [EPR26379-107] (AB300459)")
![Western blot - Anti-IRF4 antibody [EPR26379-107] (AB300459), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-epr26379-107-ab300459--western-blot-img441104.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IRF4 antibody [EPR26379-107] (AB300459)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IHC-Fr | ICC/IF | Flow Cyt | IP | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Target data
Function
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes.
Alternative names
Spip, Irf4, Interferon regulatory factor 4, IRF-4, Lymphocyte-specific interferon regulatory factor, NF-EM5, PU.1 interaction partner, Transcriptional activator PIP, LSIRF
Recommended products
Rabbit Monoclonal IRF4 antibody. Suitable for WB, IP and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR26379-107
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The IRF4 protein also known as Interferon Regulatory Factor 4 is a transcription factor encoded by the IRF4 gene. It weighs approximately 52 kDa. IRF4 is expressed mainly in lymphoid cells including B cells T cells and macrophages. It plays a mechanical role in regulating gene expression by binding to specific DNA sequences. Through these interactions IRF4 influences various cellular processes important for the immune response.
- Biological function summary
IRF4 helps in the activation and differentiation of immune cells. It forms complexes with other transcription factors like PU.1 enhancing its regulatory functions. IRF4 also contributes to the maturation of B and T lymphocytes impacting the adaptive immune system. It acts by modulating expression of cytokines and surface markers involved in immune response.
- Pathways
IRF4 functions within the immune response and cell differentiation pathways. It interacts with pathways like NF-kB and MAPK linking it to important immune regulatory processes. IRF4 associates with proteins such as c-Rel in the NF-kB pathway influencing gene expression related to immune activation and survival.
- Associated diseases and disorders
IRF4 plays a role in multiple myeloma and autoimmune disorders. Aberrant expression of IRF4 can lead to dysregulated immune cell proliferation as seen in multiple myeloma. It also connects with proteins like BLIMP-1 contributing to pathogenesis of autoimmune conditions. Understanding IRF4’s involvement provides insights into potential therapeutic targets for such diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-IRF4 antibody [EPR26379-107] (ab300459), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-epr26379-107-ab300459--immunoprecipitation-img400849.jpg "Immunoprecipitation - Anti-IRF4 antibody [EPR26379-107] (ab300459)")
Immunoprecipitation - Anti-IRF4 antibody [EPR26379-107] (ab300459)
IRF4 was immunoprecipitated from 0.35 mg A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate 10 ug with ab300459 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300459 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate 10 ug Lane 2: ab300459 IP in A20 whole cell lysate Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300459 in A20 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes
Lane 1: Immunoprecipitation - Anti-IRF4 antibody [EPR26379-107] (ab300459) at 1/30 dilution
Lane 2: Immunoprecipitation - Anti-IRF4 antibody [EPR26379-107] (ab300459) at 1/1000 dilution
All lanes: A20 whole cell lysate at 10 µg
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3min
![Western blot - Anti-IRF4 antibody [EPR26379-107] (ab300459), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-epr26379-107-ab300459--western-blot-img441104.jpg "Western blot - Anti-IRF4 antibody [EPR26379-107] (ab300459)")
Western blot - Anti-IRF4 antibody [EPR26379-107] (ab300459)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: RAW264.7 (PMID: 16243976).
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-IRF4 antibody [EPR26379-107] (ab300459) at 1/1000 dilution
Lane 1: A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate at 20 µg
Lane 2: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 52 kDa
Observed band size: 51 kDa
Exposure time: 3min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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