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Rabbit Recombinant Monoclonal IRF4 antibody. Suitable for IHC-P, IP, ChIP, WB and reacts with Human samples. Cited in 3 publications.


Images

ChIP - Anti-IRF4 antibody [EPR5653] (AB124691), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EPR5653] (AB124691), expandable thumbnail
  • Immunoprecipitation - Anti-IRF4 antibody [EPR5653] (AB124691), expandable thumbnail
  • Western blot - Anti-IRF4 antibody [EPR5653] (AB124691), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EPR5653] (AB124691), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPChIPFlow CytWB
Human
Tested
Not recommended
Tested
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg for 25.00000 µg chromatin
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity).

Alternative names

MUM1, IRF4, Interferon regulatory factor 4, IRF-4, Lymphocyte-specific interferon regulatory factor, Multiple myeloma oncogene 1, NF-EM5, LSIRF

Recommended products

Rabbit Recombinant Monoclonal IRF4 antibody. Suitable for IHC-P, IP, ChIP, WB and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5653
Purification technique
Affinity purification Protein A
Specificity

Uniprot ID - Q15306

Dissociation constant
4.18 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The IRF4 protein also known as Interferon Regulatory Factor 4 is a transcription factor encoded by the IRF4 gene. It weighs approximately 52 kDa. IRF4 is expressed mainly in lymphoid cells including B cells T cells and macrophages. It plays a mechanical role in regulating gene expression by binding to specific DNA sequences. Through these interactions IRF4 influences various cellular processes important for the immune response.

Biological function summary

IRF4 helps in the activation and differentiation of immune cells. It forms complexes with other transcription factors like PU.1 enhancing its regulatory functions. IRF4 also contributes to the maturation of B and T lymphocytes impacting the adaptive immune system. It acts by modulating expression of cytokines and surface markers involved in immune response.

Pathways

IRF4 functions within the immune response and cell differentiation pathways. It interacts with pathways like NF-kB and MAPK linking it to important immune regulatory processes. IRF4 associates with proteins such as c-Rel in the NF-kB pathway influencing gene expression related to immune activation and survival.

Associated diseases and disorders

IRF4 plays a role in multiple myeloma and autoimmune disorders. Aberrant expression of IRF4 can lead to dysregulated immune cell proliferation as seen in multiple myeloma. It also connects with proteins like BLIMP-1 contributing to pathogenesis of autoimmune conditions. Understanding IRF4’s involvement provides insights into potential therapeutic targets for such diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • ChIP - Anti-IRF4 antibody [EPR5653] (ab124691), expandable thumbnail

    ChIP - Anti-IRF4 antibody [EPR5653] (ab124691)

    Chromatin was prepared from SK-MEL-28 cells according to the Abcam Dual-X-ChIP protocol. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 μg of chromatin, 5 μg of ab124691 (red), or 5 μg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 25 μl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

    ChIP was performed according to the literature (PMID: 24267888)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EPR5653] (ab124691), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EPR5653] (ab124691)

    ab124691, at 1/100, staining IRF4 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-IRF4 antibody [EPR5653] (ab124691), expandable thumbnail

    Immunoprecipitation - Anti-IRF4 antibody [EPR5653] (ab124691)

    IRF4 was immunoprecipitated from 0.35 mg Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg with 124691 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg

    Lane 2: ab124691 IP in Ramos whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab124691 in Ramos whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-IRF4 antibody [EPR5653] (ab124691)

    Predicted band size: 52 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-IRF4 antibody [EPR5653] (ab124691), expandable thumbnail

    Western blot - Anti-IRF4 antibody [EPR5653] (ab124691)

    All lanes: Western blot - Anti-IRF4 antibody [EPR5653] (ab124691) at 1/1000 dilution

    Lane 1: Ramos cell lysate at 10 µg

    Lane 2: HuT-78 cell lysate at 10 µg

    Lane 3: IM 9 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EPR5653] (ab124691), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EPR5653] (ab124691)

    ab124691, at 1/100, staining IRF4 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EPR5653] (ab124691), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EPR5653] (ab124691)

    Immunohistochemical analysis of dog spleen tissue, staining IRF4 with ab124691.

    Tissue was fixed with formaldehyde and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a TRIS-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/50 in PBS plus 1X casein) for 1 hour 30 minutes at 37°C. An undiluted HRP-conjugated horse anti-rabbit polyclonal IgG was used as the secondary antibody.

  • OI-RD Scanning - Anti-IRF4 antibody [EPR5653] (ab124691), expandable thumbnail

    OI-RD Scanning - Anti-IRF4 antibody [EPR5653] (ab124691)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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