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AB12501

Anti-IRF4 antibody [MUM1p]

4

(1 Review)

|

(6 Publications)

Mouse Monoclonal IRF4 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IRF4.

View Alternative Names

MUM1, IRF4, Interferon regulatory factor 4, IRF-4, Lymphocyte-specific interferon regulatory factor, Multiple myeloma oncogene 1, NF-EM5, LSIRF

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [MUM1p] (AB12501)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [MUM1p] (AB12501)

IHC image of ab12501 staining in human Hodgkin's lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12501, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MUM1p

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IRF4.

Q15306

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/25", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: Tissue culture supernatant, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IRF4 protein also known as Interferon Regulatory Factor 4 is a transcription factor encoded by the IRF4 gene. It weighs approximately 52 kDa. IRF4 is expressed mainly in lymphoid cells including B cells T cells and macrophages. It plays a mechanical role in regulating gene expression by binding to specific DNA sequences. Through these interactions IRF4 influences various cellular processes important for the immune response.
Biological function summary

IRF4 helps in the activation and differentiation of immune cells. It forms complexes with other transcription factors like PU.1 enhancing its regulatory functions. IRF4 also contributes to the maturation of B and T lymphocytes impacting the adaptive immune system. It acts by modulating expression of cytokines and surface markers involved in immune response.

Pathways

IRF4 functions within the immune response and cell differentiation pathways. It interacts with pathways like NF-kB and MAPK linking it to important immune regulatory processes. IRF4 associates with proteins such as c-Rel in the NF-kB pathway influencing gene expression related to immune activation and survival.

IRF4 plays a role in multiple myeloma and autoimmune disorders. Aberrant expression of IRF4 can lead to dysregulated immune cell proliferation as seen in multiple myeloma. It also connects with proteins like BLIMP-1 contributing to pathogenesis of autoimmune conditions. Understanding IRF4’s involvement provides insights into potential therapeutic targets for such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes.
See full target information IRF4
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 29:60 PubMed37098476

2023

Exosomal IRF1-loaded rat adipose-derived stem cell sheet contributes to wound healing in the diabetic foot ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wu,Jun Tu,Jinjun Huang,Huicai Wen,Yuanlin Zeng,Yingjie Lu

Lupus science & medicine 6:e000324 PubMed31205728

2019

Cell of origin in diffuse large B-cell lymphoma in systemic lupus erythematosus: molecular and clinical factors associated with survival.

Applications

Unspecified application

Species

Unspecified reactive species

Basile Tessier-Cloutier,David Dw Twa,Eva Baecklund,Randy Gascoyne,Nathalie A Johnson,Carin Backlin,Diane L Kamen,Ann E Clarke,Rosalind Ramsey-Goldman,Jennifer Lf Lee,Pedro Farinha,Sasha Bernatsky

Neuro-oncology 16:728-34 PubMed24497406

2014

Different spatial distribution between germinal center B and non-germinal center B primary central nervous system lymphoma revealed by magnetic resonance group analysis.

Applications

Unspecified application

Species

Human

Manabu Kinoshita,Takashi Sasayama,Yoshitaka Narita,Fumio Yamashita,Atsushi Kawaguchi,Yasuyoshi Chiba,Naoki Kagawa,Kazuhiro Tanaka,Eiji Kohmura,Hideyuki Arita,Yoshiko Okita,Makoto Ohno,Yasuji Miyakita,Soichiro Shibui,Naoya Hashimoto,Toshiki Yoshimine

Archives of pathology & laboratory medicine 134:449-56 PubMed20196672

2010

Activation-induced cytidine deaminase expression in diffuse large B-cell lymphoma with a paracortical growth pattern: a lymphoma of possible interfollicular large B-cell origin.

Applications

IHC-P

Species

Human

Nadine S Aguilera,Aaron Auerbach,Carol L Barekman,Jack Lichy,Susan L Abbondanzo

Leukemia & lymphoma 48:2221-32 PubMed17926183

2007

Protein expression and cellular localization in two prognostic subgroups of diffuse large B-cell lymphoma: higher expression of ZAP70 and PKC-beta II in the non-germinal center group and poor survival in patients deficient in nuclear PTEN.

Applications

IHC-P

Species

Human

Marie Fridberg,Anna Servin,Lola Anagnostaki,Johan Linderoth,Mattias Berglund,Ola Söderberg,Gunilla Enblad,Anders Rosén,Tomas Mustelin,Mats Jerkeman,Jenny L Persson,Anette Gjörloff Wingren

Cancer science 97:1050-5 PubMed16918993

2006

Clinico-pathological characteristics of p63 expression in B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Noriyasu Fukushima,Toshimi Satoh,Naoko Sueoka,Akemi Sato,Masaru Ide,Takashi Hisatomi,Nobuo Kuwahara,Rika Tomimasu,Naoko Tsuneyoshi,Noriko Funai,Masayuki Sano,Osamu Tokunaga,Eisaburo Sueoka
View all publications
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Product promise

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