Skip to main content

Mouse Monoclonal IRF4 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IRF4.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [MUM1p] (AB12501), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.3 - 7.5
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human IRF4. Database link Q15306

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/10.00000 - 1/25.00000
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity).

Alternative names

MUM1, IRF4, Interferon regulatory factor 4, IRF-4, Lymphocyte-specific interferon regulatory factor, Multiple myeloma oncogene 1, NF-EM5, LSIRF

Recommended products

Mouse Monoclonal IRF4 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IRF4.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human IRF4. Database link Q15306
Clone number
MUM1p
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The IRF4 protein also known as Interferon Regulatory Factor 4 is a transcription factor encoded by the IRF4 gene. It weighs approximately 52 kDa. IRF4 is expressed mainly in lymphoid cells including B cells T cells and macrophages. It plays a mechanical role in regulating gene expression by binding to specific DNA sequences. Through these interactions IRF4 influences various cellular processes important for the immune response.

Biological function summary

IRF4 helps in the activation and differentiation of immune cells. It forms complexes with other transcription factors like PU.1 enhancing its regulatory functions. IRF4 also contributes to the maturation of B and T lymphocytes impacting the adaptive immune system. It acts by modulating expression of cytokines and surface markers involved in immune response.

Pathways

IRF4 functions within the immune response and cell differentiation pathways. It interacts with pathways like NF-kB and MAPK linking it to important immune regulatory processes. IRF4 associates with proteins such as c-Rel in the NF-kB pathway influencing gene expression related to immune activation and survival.

Associated diseases and disorders

IRF4 plays a role in multiple myeloma and autoimmune disorders. Aberrant expression of IRF4 can lead to dysregulated immune cell proliferation as seen in multiple myeloma. It also connects with proteins like BLIMP-1 contributing to pathogenesis of autoimmune conditions. Understanding IRF4’s involvement provides insights into potential therapeutic targets for such diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [MUM1p] (ab12501), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [MUM1p] (ab12501)

    IHC image of ab12501 staining in human Hodgkin's lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12501, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com