Rabbit Recombinant Monoclonal IRF4 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (AB242427), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-bsa-and-azide-free-ab242427--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15697.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (AB242427)")
![Flow Cytometry (Intracellular) - Anti-IRF4 antibody [SP114] - BSA and Azide free (AB242427), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-bsa-and-azide-free-ab242427--flow-cytometry-intracellular-img38573.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IRF4 antibody [SP114] - BSA and Azide free (AB242427)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (AB242427), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-bsa-and-azide-free-ab242427--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112794.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (AB242427)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (AB242427), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-bsa-and-azide-free-ab242427--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137128.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (AB242427)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (AB242427), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-bsa-and-azide-free-ab242427--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81234.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (AB242427)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | IP | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested |
| Mouse | Predicted | Not recommended | Predicted | Predicted |
| Rat | Predicted | Not recommended | Predicted | Predicted |
| Chicken | Predicted | Not recommended | Predicted | Predicted |
| Cow | Predicted | Not recommended | Predicted | Predicted |
| Dog | Predicted | Not recommended | Predicted | Predicted |
| Rabbit | Predicted | Not recommended | Predicted | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Mouse, Rat, Rabbit | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Not suitable for IP-Mouse. |
Species Chicken | Dilution info - | Notes - |
Species Cow | Dilution info - | Notes - |
Species Dog | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Rabbit | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Primary antibody incubation for 1 hour at room temperature. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Mouse, Rat, Rabbit | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat mediated antigen retrieval with 10 mM EDTA buffer before commencing with IHC staining protocol. Primary antibody incubation for 30 minutes at room temperature. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Mouse, Rat, Rabbit | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
Target data
Function
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity).
Alternative names
MUM1, IRF4, Interferon regulatory factor 4, IRF-4, Lymphocyte-specific interferon regulatory factor, Multiple myeloma oncogene 1, NF-EM5, LSIRF
Recommended products
Rabbit Recombinant Monoclonal IRF4 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- SP114
- Purification technique
- Affinity purification Protein A/G
- Specificity
Not suitable for IP-Mouse.
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab242427 is the carrier-free version of Anti-IRF4 antibody [SP114] - C-terminal ab227646.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The IRF4 protein also known as Interferon Regulatory Factor 4 is a transcription factor encoded by the IRF4 gene. It weighs approximately 52 kDa. IRF4 is expressed mainly in lymphoid cells including B cells T cells and macrophages. It plays a mechanical role in regulating gene expression by binding to specific DNA sequences. Through these interactions IRF4 influences various cellular processes important for the immune response.
- Biological function summary
IRF4 helps in the activation and differentiation of immune cells. It forms complexes with other transcription factors like PU.1 enhancing its regulatory functions. IRF4 also contributes to the maturation of B and T lymphocytes impacting the adaptive immune system. It acts by modulating expression of cytokines and surface markers involved in immune response.
- Pathways
IRF4 functions within the immune response and cell differentiation pathways. It interacts with pathways like NF-kB and MAPK linking it to important immune regulatory processes. IRF4 associates with proteins such as c-Rel in the NF-kB pathway influencing gene expression related to immune activation and survival.
- Associated diseases and disorders
IRF4 plays a role in multiple myeloma and autoimmune disorders. Aberrant expression of IRF4 can lead to dysregulated immune cell proliferation as seen in multiple myeloma. It also connects with proteins like BLIMP-1 contributing to pathogenesis of autoimmune conditions. Understanding IRF4’s involvement provides insights into potential therapeutic targets for such diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (ab242427), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-bsa-and-azide-free-ab242427--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15697.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (ab242427)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (ab242427)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling IRF4 with Anti-IRF4 antibody [SP114] - C-terminal ab227646 at 1/100 dilution (2.32 μg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF4 antibody [SP114] - C-terminal ab227646)![Flow Cytometry (Intracellular) - Anti-IRF4 antibody [SP114] - BSA and Azide free (ab242427), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-bsa-and-azide-free-ab242427--flow-cytometry-intracellular-img38573.jpg "Flow Cytometry (Intracellular) - Anti-IRF4 antibody [SP114] - BSA and Azide free (ab242427)")
Flow Cytometry (Intracellular) - Anti-IRF4 antibody [SP114] - BSA and Azide free (ab242427)
Flow Cytometry analysis of Ramos(Human Burkitt's lymphoma B lymphocyte) cells labeling IRF4 with purified Anti-IRF4 antibody [SP114] - C-terminal ab227646 at 1:230 dilution (1.01 μg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S100 beta antibody [SP127] ab115803).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (ab242427), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-bsa-and-azide-free-ab242427--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112794.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (ab242427)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (ab242427)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin's lymphoma tissue sections labeling IRF4 with Anti-IRF4 antibody [SP114] - C-terminal ab227646 at 1/100 dilution (2.32 μg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF4 antibody [SP114] - C-terminal ab227646)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (ab242427), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-bsa-and-azide-free-ab242427--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137128.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (ab242427)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (ab242427)
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for IRF4 using Anti-IRF4 antibody [SP114] - C-terminal ab227646 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-IRF4 antibody [SP114] - C-terminal ab227646).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (ab242427), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-bsa-and-azide-free-ab242427--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81234.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (ab242427)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - BSA and Azide free (ab242427)
Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for IRF4 using Anti-IRF4 antibody [SP114] - C-terminal ab227646 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-IRF4 antibody [SP114] - C-terminal ab227646).
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com