Rabbit Recombinant Monoclonal IRF4 antibody. C-terminal. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples. Cited in 2 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (AB227646), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-c-terminal-ab227646--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15697.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (AB227646)")
![Flow Cytometry (Intracellular) - Anti-IRF4 antibody [SP114] - C-terminal (AB227646), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-c-terminal-ab227646--flow-cytometry-intracellular-img38573.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IRF4 antibody [SP114] - C-terminal (AB227646)")
![Western blot - Anti-IRF4 antibody [SP114] - C-terminal (AB227646), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-c-terminal-ab227646--western-blot-img58841.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IRF4 antibody [SP114] - C-terminal (AB227646)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (AB227646), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-c-terminal-ab227646--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112794.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (AB227646)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (AB227646), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-c-terminal-ab227646--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137128.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (AB227646)")
Publications
Filter by
- Cell death and differentiation 26:2637-26512019MiR-35 buffers apoptosis thresholds in the C. elegans germline by antagonizing both MAPK and core apoptosis pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnh T Tran,Eric M Chapman,Mathieu N Flamand,Bin Yu,Samuel J Krempel,Thomas F Duchaine,Matthew Eroglu,W Brent DerryPubMed 30952991
- Virchows Archiv : an international journal of path 473:341-3492018Round-robin test for the cell-of-origin classification of diffuse large B-cell lymphoma-a feasibility study using full slide staining.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Reinke et. al.PubMed 29730836
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | WB | IHC-P | IP | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended |
| Mouse | Predicted | Predicted | Predicted | Not recommended |
| Rat | Predicted | Predicted | Predicted | Not recommended |
| Chicken | Predicted | Predicted | Predicted | Not recommended |
| Cow | Predicted | Predicted | Predicted | Not recommended |
| Dog | Predicted | Predicted | Predicted | Not recommended |
| Rabbit | Predicted | Predicted | Predicted | Not recommended |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/230 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Chicken, Cow, Dog | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Primary antibody incubation for 1 hour at room temperature. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Chicken, Cow, Dog | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat mediated antigen retrieval with 10 mM EDTA buffer before commencing with IHC staining protocol. Primary antibody incubation for 30 minutes at room temperature. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Chicken, Cow, Dog | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Not suitable for IP-Mouse. |
Species Rat | Dilution info - | Notes - |
Species Rabbit | Dilution info - | Notes - |
Species Chicken | Dilution info - | Notes - |
Species Cow | Dilution info - | Notes - |
Species Dog | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
1 product for Alternative Version
Target data
Function
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity).
Alternative names
MUM1, IRF4, Interferon regulatory factor 4, IRF-4, Lymphocyte-specific interferon regulatory factor, Multiple myeloma oncogene 1, NF-EM5, LSIRF
Recommended products
Rabbit Recombinant Monoclonal IRF4 antibody. C-terminal. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- SP114
- Purification technique
- Affinity purification Protein A/G
- Specificity
Not suitable for IP-Mouse.
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The IRF4 protein also known as Interferon Regulatory Factor 4 is a transcription factor encoded by the IRF4 gene. It weighs approximately 52 kDa. IRF4 is expressed mainly in lymphoid cells including B cells T cells and macrophages. It plays a mechanical role in regulating gene expression by binding to specific DNA sequences. Through these interactions IRF4 influences various cellular processes important for the immune response.
- Biological function summary
IRF4 helps in the activation and differentiation of immune cells. It forms complexes with other transcription factors like PU.1 enhancing its regulatory functions. IRF4 also contributes to the maturation of B and T lymphocytes impacting the adaptive immune system. It acts by modulating expression of cytokines and surface markers involved in immune response.
- Pathways
IRF4 functions within the immune response and cell differentiation pathways. It interacts with pathways like NF-kB and MAPK linking it to important immune regulatory processes. IRF4 associates with proteins such as c-Rel in the NF-kB pathway influencing gene expression related to immune activation and survival.
- Associated diseases and disorders
IRF4 plays a role in multiple myeloma and autoimmune disorders. Aberrant expression of IRF4 can lead to dysregulated immune cell proliferation as seen in multiple myeloma. It also connects with proteins like BLIMP-1 contributing to pathogenesis of autoimmune conditions. Understanding IRF4’s involvement provides insights into potential therapeutic targets for such diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-c-terminal-ab227646--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15697.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling IRF4 with ab227646 at 1/100 dilution (2.32 μg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Flow Cytometry (Intracellular) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-c-terminal-ab227646--flow-cytometry-intracellular-img38573.jpg "Flow Cytometry (Intracellular) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)")
Flow Cytometry (Intracellular) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)
Flow Cytometry analysis of Ramos(Human Burkitt's lymphoma B lymphocyte) cells labeling IRF4 with purified ab227646 at 1:230 dilution (1.01 μg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
![Western blot - Anti-IRF4 antibody [SP114] - C-terminal (ab227646), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-c-terminal-ab227646--western-blot-img58841.jpg "Western blot - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)")
Western blot - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)
Blocking/Diluting buffer and concentration: 5% NFDM /TBST
All lanes: Western blot - Anti-IRF4 antibody [SP114] - C-terminal (ab227646) at 0.25 µg/mL
Lane 1: Ramos (Human Burkitt's lymphoma B lymphocyte) cell lysate at 20 µg
Lane 2: HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) cell lysate at 20 µg
Lane 3: IM-9 (Human peripheral blood lymphoblast) cell lysate at 20 µg
Lane 4: 293T (Human embryonic kidney epithelial cell) cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 0.05 µg/mL
Predicted band size: 52 kDa
Observed band size: 52 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-c-terminal-ab227646--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112794.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin's lymphoma tissue sections labeling IRF4 with ab227646 at 1/100 dilution (2.32 μg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-c-terminal-ab227646--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137128.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for IRF4 using ab227646 at 1/100 dilution in immunohistochemical analysis.
![Western blot - Anti-IRF4 antibody [SP114] - C-terminal (ab227646), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-c-terminal-ab227646--western-blot-img94271.jpg "Western blot - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)")
Western blot - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)
All lanes: Western blot - Anti-IRF4 antibody [SP114] - C-terminal (ab227646) at 1/100 dilution
All lanes: Ramos (human Burkitt's lymphoma cell line) cell lysate
Predicted band size: 52 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-c-terminal-ab227646--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81234.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)
Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for IRF4 using ab227646 at 1/100 dilution in immunohistochemical analysis.
![Western blot - Anti-IRF4 antibody [SP114] - C-terminal (ab227646), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-c-terminal-ab227646--western-blot-img94271.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646), expandable thumbnail](https://content.abcam.com/products/images/irf4-antibody-sp114-c-terminal-ab227646--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81234.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [SP114] - C-terminal (ab227646)")
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