Anti-IRF5 antibody (ab21689) is a rabbit polyclonal antibody detecting IRF5 in Western Blot. Suitable for Human, Mouse, Rat.
- Over 20 publications
- Trusted since 2005
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- Nature communications 13:67392022CDKN1A is a target for phagocytosis-mediated cellular immunotherapy in acute leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAwatef Allouch,Laurent Voisin,Yanyan Zhang,Syed Qasim Raza,Yann Lecluse,Julien Calvo,Dorothée Selimoglu-Buet,Stéphane de Botton,Fawzia Louache,Françoise Pflumio,Eric Solary,Jean-Luc PerfettiniPubMed 36347876
- Nature communications 12:67022021Defactinib inhibits PYK2 phosphorylation of IRF5 and reduces intestinal inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGrigory Ryzhakov,Hannah Almuttaqi,Alastair L Corbin,Dorothée L Berthold,Tariq Khoyratty,Hayley L Eames,Samuel Bullers,Claire Pearson,Zhichao Ai,Kristina Zec,Sarah Bonham,Roman Fischer,Luke Jostins-Dean,Simon P L Travis,Benedikt M Kessler,Irina A UdalovaPubMed 34795257
- Molecular therapy. Nucleic acids 25:708-7152021siRNA-loaded biodegradable lipid nanoparticles ameliorate concanavalin A-induced liver injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWataru Kawase,Daisuke Kurotaki,Yuta Suzuki,Hiroshi Ishihara,Tatsuma Ban,Go R Sato,Juri Ichikawa,Hideyuki Yanai,Tadatsugu Taniguchi,Kappei Tsukahara,Tomohiko TamuraPubMed 34589288
- Nature communications 12:48132021Altered function and differentiation of age-associated B cells contribute to the female bias in lupus mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdd Ricker,Michela Manni,Danny Flores-Castro,Daniel Jenkins,Sanjay Gupta,Juan Rivera-Correa,Wenzhao Meng,Aaron M Rosenfeld,Tania Pannellini,Mahesh Bachu,Yurii Chinenov,Peter K Sculco,Rolf Jessberger,Eline T Luning Prak,Alessandra B PernisPubMed 34376664
- Nature communications 12:43792021Genetic and chemical inhibition of IRF5 suppresses pre-existing mouse lupus-like disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatsuma Ban,Masako Kikuchi,Go R Sato,Akio Manabe,Noriko Tagata,Kayo Harita,Akira Nishiyama,Kenichi Nishimura,Ryusuke Yoshimi,Yohei Kirino,Hideyuki Yanai,Yoshiko Matsumoto,Shuichi Suzuki,Hiroe Hihara,Masashi Ito,Kappei Tsukahara,Kentaro Yoshimatsu,Tadashi Yamamoto,Tadatsugu Taniguchi,Hideaki Nakajima,Shuichi Ito,Tomohiko TamuraPubMed 34282144
- Science advances 7:2021TRAF6-IRF5 kinetics, TRIF, and biophysical factors drive synergistic innate responses to particle-mediated MPLA-CpG co-presentation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesP Pradhan et. al.PubMed 33523878
- Immunity 54:235-246.e52020The persistence of interleukin-6 is regulated by a blood buffer system derived from dendritic cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshraf S Yousif,Larance Ronsard,Pankaj Shah,Tatsushi Omatsu,Maya Sangesland,Thalia Bracamonte Moreno,Evan C Lam,Vladimir D Vrbanac,Alejandro B Balazs,Hans-Christian Reinecker,Daniel LingwoodPubMed 33357409
- EMBO molecular medicine 12:e130382020Monocytopenia, monocyte morphological anomalies and hyperinflammation characterise severe COVID-19 in type 2 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFawaz Alzaid,Jean-Baptiste Julla,Marc Diedisheim,Charline Potier,Louis Potier,Gilberto Velho,Bénédicte Gaborit,Philippe Manivet,Stéphane Germain,Tiphaine Vidal-Trecan,Ronan Roussel,Jean-Pierre Riveline,Elise Dalmas,Nicolas Venteclef,Jean-François GautierPubMed 32816392
- Nucleic acids research 48:589-6042019Specific enhancer selection by IRF3, IRF5 and IRF9 is determined by ISRE half-sites, 5' and 3' flanking bases, collaborating transcription factors and the chromatin environment in a combinatorial fashion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMária Csumita,Attila Csermely,Attila Horvath,Gergely Nagy,Fanny Monori,Loránd Göczi,Hans-Acha Orbea,Walter Reith,Lajos SzélesPubMed 31799619
- Cell reports 28:3367-3380.e82019GEF-H1 Signaling upon Microtubule Destabilization Is Required for Dendritic Cell Activation and Specific Anti-tumor Responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbhishek S Kashyap,Laura Fernandez-Rodriguez,Yun Zhao,Gianni Monaco,Marcel P Trefny,Naohiro Yoshida,Kea Martin,Ashwani Sharma,Natacha Olieric,Pankaj Shah,Michal Stanczak,Nicole Kirchhammer,Sung-Moo Park,Sebastien Wieckowski,Heinz Laubli,Rachid Zagani,Benjamin Kasenda,Michel O Steinmetz,Hans-Christian Reinecker,Alfred ZippeliusPubMed 31553907
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Not recommended |
| Mouse | Tested | Not recommended |
| Rat | Tested | Not recommended |
| Cow | Predicted | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PubMed:11303025, PubMed:15695821, PubMed:22412986, PubMed:25326418, PubMed:32433612). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (By similarity). Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway (By similarity). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
Alternative names
Interferon regulatory factor 5, IRF-5, IRF5
Recommended products
Anti-IRF5 antibody (ab21689) is a rabbit polyclonal antibody detecting IRF5 in Western Blot. Suitable for Human, Mouse, Rat.
- Over 20 publications
- Trusted since 2005
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-IRF5 antibody (ab21689) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.
What is the molecular weight of IRF5?
Anti-IRF5 (ab21689) specifically detects a band for IRF5 (UniProt: Q13568) at a molecular weight of 58kDa.
Trusted by the scientific community
Anti-IRF5 (ab21689) was first used in a scientific publication in 2005 and has been cited over 20 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IRF5 or Interferon Regulatory Factor 5 is a member of the IRF family of transcription factors. This protein weighs approximately 56 kDa and is expressed in cells of the immune system including B cells dendritic cells and macrophages. IRF5 plays an important role in regulating the transcription of interferon-stimulated genes following a viral infection. It acts by binding to specific DNA sequences initiating transcription processes that are important for the antiviral response.
- Biological function summary
IRF5 influences the immune response through the regulation of cytokine production. It activates genes involved in producing pro-inflammatory cytokines such as TNF-α and IL-6 which are important for clearing viral infections and activating immune cells. IRF5 does not usually form part of a protein complex but it interacts closely with TRAF6 and MyD88 for its activation. Its pivotal role in cytokine production highlights its importance in controlling inflammation.
- Pathways
IRF5 is an important component of the Toll-like receptor (TLR) signaling pathway and plays a role in the MyD88-dependent pathway. In this context IRF5 gets activated by TLRs interacting with MyD88 and TAK1 to enhance the transcription of important genes for immune response. It is also closely connected to the IRF3 protein which shares similar activation pathways and can similarly initiate an antiviral state.
- Associated diseases and disorders
Defects or dysregulation in IRF5 function are linked to autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE IRF5 influences autoantibody production contributing to disease development. It works alongside other regulatory proteins involved in autoimmunity including IFN-α and STAT4 which also play significant roles in modulating immune responses. Understanding IRF5's role in these conditions could present new opportunities for therapeutic interventions using IRF5 inhibitors.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Western blot - Anti-IRF5 antibody (ab21689)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab21689 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-IRF5 antibody (ab21689) at 1 µg/mL
Lane 1: HeLa Whole Cell Lysate at 20 µg
Lane 2: Mouse Liver Tissue Lysate at 10 µg
Lane 3: Mouse Kidney Tissue Lysate at 10 µg
Lane 4: Rat Liver Tissue Lysate at 10 µg
SecondaryAll lanes: Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 25 kDa, 60 kDa, 85 kDa
Exposure time: 8min
Western blot - Anti-IRF5 antibody (ab21689)
All lanes: Western blot - Anti-IRF5 antibody (ab21689) at 1 µg/mL
Lane 1: HeLa whole cell lysate at 20 µg
Lane 2: HeLa whole cell lysate at 20 µg with Human IRF5 peptide (ab22411)
SecondaryAll lanes: Alexa Fluor Goat polyclonal to IgG (700) at 1/10000 dilution
Predicted band size: 56 kDa
Observed band size: 60 kDa
Western blot - Anti-IRF5 antibody (ab21689)
All lanes: Western blot - Anti-IRF5 antibody (ab21689) at 1 µg/mL
Lane 1: Liver (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 2: Kidney (Mouse) Tissue Lysate at 10 µg
Lane 3: Liver (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 60 kDa
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