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Rabbit Recombinant Monoclonal IRF5 phospho S446 antibody. Suitable for WB, Dot and reacts with Human, Transfected cell lysate - Human, Synthetic peptide samples.

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Images

Western blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (AB309088), expandable thumbnail
  • Western blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (AB309088), expandable thumbnail
  • Dot Blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (AB309088), expandable thumbnail
  • Western blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (AB309088), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBDotICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Not recommended
Not recommended
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell lysate - Human, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell lysate - Human, Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PubMed:11303025, PubMed:15695821, PubMed:22412986, PubMed:25326418, PubMed:32433612). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (By similarity). Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway (By similarity). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).

Alternative names

Interferon regulatory factor 5, IRF-5, IRF5

Recommended products

Rabbit Recombinant Monoclonal IRF5 phospho S446 antibody. Suitable for WB, Dot and reacts with Human, Transfected cell lysate - Human, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26132-254
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

IRF5 or Interferon Regulatory Factor 5 is a member of the IRF family of transcription factors. This protein weighs approximately 56 kDa and is expressed in cells of the immune system including B cells dendritic cells and macrophages. IRF5 plays an important role in regulating the transcription of interferon-stimulated genes following a viral infection. It acts by binding to specific DNA sequences initiating transcription processes that are important for the antiviral response.

Biological function summary

IRF5 influences the immune response through the regulation of cytokine production. It activates genes involved in producing pro-inflammatory cytokines such as TNF-α and IL-6 which are important for clearing viral infections and activating immune cells. IRF5 does not usually form part of a protein complex but it interacts closely with TRAF6 and MyD88 for its activation. Its pivotal role in cytokine production highlights its importance in controlling inflammation.

Pathways

IRF5 is an important component of the Toll-like receptor (TLR) signaling pathway and plays a role in the MyD88-dependent pathway. In this context IRF5 gets activated by TLRs interacting with MyD88 and TAK1 to enhance the transcription of important genes for immune response. It is also closely connected to the IRF3 protein which shares similar activation pathways and can similarly initiate an antiviral state.

Associated diseases and disorders

Defects or dysregulation in IRF5 function are linked to autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE IRF5 influences autoantibody production contributing to disease development. It works alongside other regulatory proteins involved in autoimmunity including IFN-α and STAT4 which also play significant roles in modulating immune responses. Understanding IRF5's role in these conditions could present new opportunities for therapeutic interventions using IRF5 inhibitors.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (ab309088), expandable thumbnail
    The image was kindly provided by an anonymous collaborator.

    Western blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (ab309088)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (ab309088) at 1/500 dilution

    Lane 1: Untreated THP-1 (human monocytic leukemia monocyte) overexpressing TLR9 whole cell lysate at 25 µg

    Lane 2: THP-1 overexpressing TLR9 treated with CpG-B for 3 hours whole cell lysate at 25 µg

    Lane 3: Untreated IRF5 knockout THP-1 overexpressing TLR9 whole cell lysate at 25 µg

    Lane 4: IRF5 knockout THP-1 overexpressing TLR9 treated with CpG-B for 3 hours whole cell lysate at 25 µg

    Predicted band size: 56 kDa

    Observed band size: 56 kDa

  • Western blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (ab309088), expandable thumbnail

    Western blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (ab309088)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    In Western blot, anti-IRF5 antibody (Anti-IRF5 antibody [EPR17067] ab181553) staining at 1/1000 dilution.

    Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (ab309088) at 1/1000 dilution

    Lane 1: 293T cells transfected with a human IRF5 expression vector containing a His-tag whole cell lysate (untreated membrane) at 20 µg

    Lane 2: 293T cells transfected with a human IRF5 expression vector containing a His-tag whole cell lysate (phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 56 kDa

    Exposure time: 180s

  • Dot Blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (ab309088), expandable thumbnail

    Dot Blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (ab309088)

    Dot blot analysis of IRF5 (phospho S446) using ab309088 at 1:1000 (0.503 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
    Lane 1: IRF5 (phospho S446) peptide a
    Lane 2: IRF5 (phospho S446) peptide b
    Lane 3: IRF5 (phospho S446) non-phospho peptide c
    Exposure time: 180 seconds
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (ab309088), expandable thumbnail

    Western blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (ab309088)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
    Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 26 seconds

    All lanes: Western blot - Anti-IRF5 (phospho S446) antibody [EPR26132-254] (ab309088) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containi a his tag whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human IRF5 expression vector containi a His-tag whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a human IRF5 (S446D mutation) expression vector containi a His-tag whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a human IRF5 (S446A mutation) expression vector containi a His-tag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 56 kDa

    Exposure time: 26s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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