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AB288440

Anti-IRF7 antibody [EPR25274-33]

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(1 Publication)

Rabbit Recombinant Monoclonal IRF7 antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Interferon regulatory factor 7, IRF-7, Irf7

1 Images
Western blot - Anti-IRF7 antibody [EPR25274-33] (AB288440)
  • WB

Supplier Data

Western blot - Anti-IRF7 antibody [EPR25274-33] (AB288440)

Blocking and dilution buffer : 5% NFDM/TBST

Exposure time : 3 minutes

Induction of IRF-7 expression by IFN alpha treatment (PMID : 30598516)

All lanes:

Anti-MRPL23 antibody (<a href='/en-us/products/unavailable/mrpl23-antibody-ab228440'>ab228440</a>) at 1/1000 dilution

Lane 1:

Untreated A20 (Mouse reticulum sarcoma B lymphocyte), whole cell lysate at 20 µg

Lane 2:

A20 treated with 10 ng/ml mouse IFN alpha for 24 hours, added 10 μM MG-132 for 6 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 54 kDa

Observed band size: 45-60 kDa

false

  • Carrier free

    Anti-IRF7 antibody [EPR25274-33] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25274-33

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The transcription factor IRF7 also known as Interferon Regulatory Factor 7 plays a significant role in the regulation of type I interferon genes. This protein acts as an important modulator of the immune response specifically during viral infections. The molecular weight of IRF7 is approximately 54 kDa. It is predominantly expressed in lymphoid tissues such as the spleen and thymus and is also found in other immune cells like B cells and dendritic cells. The expression of IRF7 is induced by pathogen-associated molecular patterns which stimulate the activation of immune signaling pathways.
Biological function summary

IRF7 activates and modulates the expression of type I interferons and other cytokines important for antiviral defense. This protein can form part of a larger complex with other IRF family members to enhance its effect on gene transcription. IRF7 operates through its interaction with transcriptional coactivators allowing it to execute its function as a transcriptional activator reinforcing the immune system's response to infectious agents.

Pathways

IRF7 plays a critical role in the Toll-like receptor (TLR) signaling pathway and retinoic acid-inducible gene I (RIG-I) pathway. It directly interacts with MyD88 and IRAK1 proteins linking it to signal transduction pathways that lead to interferon-beta production. The activation of IRF7 in these pathways also strengthens the body's defense mechanism by enhancing the transcription of antiviral genes forming a robust antibacterial and antiviral environment within the host.

IRF7's function is implicated in autoimmune diseases and specific cancers. For autoimmune conditions like systemic lupus erythematosus (SLE) dysregulated IRF7 activity is observed leading to an aberrant interferon response. It connects with proteins such as IRF5 which has its expressions elevated in affected patients. In relation to cancer IRF7 can impact tumor progression particularly in breast cancer through modulation of immune surveillance functions. Its interaction with interferon pathways becomes a double-edged sword affecting tumor immunity and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses (PubMed : 22095711, PubMed : 27129230). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Induces transcription of ubiquitin hydrolase USP25 mRNA in response to lipopolysaccharide (LPS) or viral infection in a type I IFN-dependent manner (PubMed : 27129230). Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.
See full target information Irf7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine 23:437-449 PubMed36336986

2022

Promotive role of IRF7 in ferroptosis of colonic epithelial cells in ulcerative colitis by the miR-375-3p/SLC11A2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zepeng Chen,Qinglong Gu,Ruichao Chen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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