JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB240752

Anti-IRF8 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal IRF8 antibody. Suitable for IP, ChIP-seq and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Irf8 aa 200-250.

View Alternative Names

Icsbp, Icsbp1, Irf8, Interferon regulatory factor 8, IRF-8, Interferon consensus sequence-binding protein, ICSBP

1 Images
ChIP-sequencing - Anti-IRF8 antibody (AB240752)
  • ChIP-seq

Supplier Data

ChIP-sequencing - Anti-IRF8 antibody (AB240752)

Chromatin from mouse bone marrow dendritic cells (BMDCs) was immunoprecipitated with ab240752 and analyzed by DNA sequencing. The figure illustrates the peak distribution of IRF8 binding within a 156 kb region of chromosome 19 (blue) as detected by ab240752.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

ChIP-seq, IP

applications

Immunogen

Synthetic Peptide within Mouse Irf8 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

P23611

style
left-column

Alternative Products

Primary Antibodies

AB245607

Anti-IRF8 antibody

Immunoprecipitation - Anti-IRF8 antibody (AB245607)

  • 0
  • 0
Primary Antibodies

AB168808

Anti-IRF8 antibody

Immunoprecipitation - Anti-IRF8 antibody (AB168808)

  • 0
  • 0
Primary Antibodies

AB207418

Anti-IRF8 antibody [EPR20441]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] (AB207418)

  • 5
  • 1

Complementary Products

Primary Antibodies

AB308516

Anti-HOXA10 antibody [EPR27317-76]

20ul selling size|RabMAb|Recombinant|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-HOXA10 antibody [EPR27317-76] (AB308516)

  • 0
  • 0
Primary Antibodies

AB92506

Anti-STAT1 alpha antibody [EPYR2154]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Advanced Validation

Immunoprecipitation - Anti-STAT1 alpha antibody [EPYR2154] (AB92506)

  • 4
  • 2
Primary Antibodies

AB109461

Anti-STAT1 (phospho S727) antibody [EPR3146]

20ul selling size|Recombinant|Advanced Validation|RabMAb|KO Validated

Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)

  • 4
  • 1
Primary Antibodies

AB227835

Anti-PU.1/Spi1 antibody [EPR22624-20] - ChIP Grade

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-PU.1/Spi1 antibody [EPR22624-20] - ChIP Grade (AB227835)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRF8 also known as Interferon Regulatory Factor 8 plays a significant role as a transcription factor in the regulation of gene expression. With a molecular mass of approximately 48 kDa this nuclear protein primarily functions in immune cells such as monocytes dendritic cells and macrophages. It binds to specific DNA sequences in gene promoters influencing the transcription of genes involved in immune response and development of blood cells. The expression of IRF8 extends beyond immune cells to tissues like the spleen and thymus.
Biological function summary

IRF8 drives differentiation and maturation of various immune cell types. It forms part of protein complexes that control the expression of genes necessary for immune surveillance and defense mechanisms. IRF8 can dimerize with other IRF family members or proteins like PU.1 to modulate activity of specific target genes. This transcription factor supports the growth and differentiation of myeloid cells and helps to balance lymphoid and myeloid lineage commitment which is important for maintaining a proper immune system function.

Pathways

IRF8 is integral to the interferon signaling and hematopoietic cell lineage pathways. It enhances the expression of interferon-stimulated genes integrating signals for a potent antiviral response. The protein connects with transcriptional regulators such as IRF1 and STAT1 to modulate these pathways. By participating in hematopoietic differentiation IRF8 also influences pathways related to the immune system development and inflammatory responses.

Mutations or alterations in IRF8 function associate with immunodeficiencies and hematologic malignancies. Chronic myelogenous leukemia and systemic lupus erythematosus exemplify conditions where IRF8 plays a role. Its dysfunction affects the regulation of cytokine genes which can lead to inappropriate immune responses. The protein's activity intersects with other key players such as BTK and BCL2 which are involved in disease processes related to immune cell proliferation and survival.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) (PubMed : 12393690, PubMed : 2111015). Can both act as a transcriptional activator or repressor (PubMed : 2111015). Plays a negative regulatory role in cells of the immune system (PubMed : 2111015). Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (PubMed : 12393690, PubMed : 22992524). Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection (PubMed : 12393690, PubMed : 12461077, PubMed : 12538667, PubMed : 23382217). Positively regulates macroautophagy in dendritic cells (By similarity). Acts as a transcriptional repressor of osteoclast differentiation factors such as NFATC1 and EEIG1 (PubMed : 32741026).
See full target information Irf8
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC developmental biology 20:22 PubMed33203369

2020

Depletion of SNRNP200 inhibits the osteo-/dentinogenic differentiation and cell proliferation potential of stem cells from the apical papilla.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomin Su,Haoqing Yang,Ruitang Shi,Chen Zhang,Huina Liu,Zhipeng Fan,Jianpeng Zhang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com