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AB245607

Anti-IRF8 antibody

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(2 Publications)

Rabbit Polyclonal IRF8 antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Irf8 aa 350 to C-terminus.

View Alternative Names

Icsbp, Icsbp1, Irf8, Interferon regulatory factor 8, IRF-8, Interferon consensus sequence-binding protein, ICSBP

3 Images
Immunoprecipitation - Anti-IRF8 antibody (AB245607)
  • IP

Supplier Data

Immunoprecipitation - Anti-IRF8 antibody (AB245607)

IRF8 was immunoprecipitated from RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245607 used for IP at 3 μg per reaction. For WB 1 μg/ml.

Lane 1 : ab245607 IP in RAW 264.7 whole cell lysate.
Lane 2 : Control IgG IP in RAW 264.7 whole cell lysate.
Chemiluminescence detection : 3 minutes.

All lanes:

Immunoprecipitation - Anti-IRF8 antibody (ab245607)

Predicted band size: 48 kDa

false

Western blot - Anti-IRF8 antibody (AB245607)
  • WB

Supplier Data

Western blot - Anti-IRF8 antibody (AB245607)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-IRF8 antibody (ab245607) at 0.1 µg/mL

Lane 1:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 50 µg

Lane 2:

RAW 264.7 whole cell lysate at 15 µg

Predicted band size: 48 kDa

true

Exposure time: 3min

Western blot - Anti-IRF8 antibody (AB245607)
  • WB

Supplier Data

Western blot - Anti-IRF8 antibody (AB245607)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-IRF8 antibody (ab245607) at 0.1 µg/mL

All lanes:

L1210 whole cell lysate at 50 µg

Predicted band size: 48 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Mouse Irf8 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P23611

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab245607 was affinity purified using an epitope specific to IRF8 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRF8 also known as Interferon Regulatory Factor 8 plays a significant role as a transcription factor in the regulation of gene expression. With a molecular mass of approximately 48 kDa this nuclear protein primarily functions in immune cells such as monocytes dendritic cells and macrophages. It binds to specific DNA sequences in gene promoters influencing the transcription of genes involved in immune response and development of blood cells. The expression of IRF8 extends beyond immune cells to tissues like the spleen and thymus.
Biological function summary

IRF8 drives differentiation and maturation of various immune cell types. It forms part of protein complexes that control the expression of genes necessary for immune surveillance and defense mechanisms. IRF8 can dimerize with other IRF family members or proteins like PU.1 to modulate activity of specific target genes. This transcription factor supports the growth and differentiation of myeloid cells and helps to balance lymphoid and myeloid lineage commitment which is important for maintaining a proper immune system function.

Pathways

IRF8 is integral to the interferon signaling and hematopoietic cell lineage pathways. It enhances the expression of interferon-stimulated genes integrating signals for a potent antiviral response. The protein connects with transcriptional regulators such as IRF1 and STAT1 to modulate these pathways. By participating in hematopoietic differentiation IRF8 also influences pathways related to the immune system development and inflammatory responses.

Mutations or alterations in IRF8 function associate with immunodeficiencies and hematologic malignancies. Chronic myelogenous leukemia and systemic lupus erythematosus exemplify conditions where IRF8 plays a role. Its dysfunction affects the regulation of cytokine genes which can lead to inappropriate immune responses. The protein's activity intersects with other key players such as BTK and BCL2 which are involved in disease processes related to immune cell proliferation and survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) (PubMed : 12393690, PubMed : 2111015). Can both act as a transcriptional activator or repressor (PubMed : 2111015). Plays a negative regulatory role in cells of the immune system (PubMed : 2111015). Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (PubMed : 12393690, PubMed : 22992524). Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection (PubMed : 12393690, PubMed : 12461077, PubMed : 12538667, PubMed : 23382217). Positively regulates macroautophagy in dendritic cells (By similarity). Acts as a transcriptional repressor of osteoclast differentiation factors such as NFATC1 and EEIG1 (PubMed : 32741026).
See full target information Irf8
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed35550030

2022

CREB5 reprograms FOXA1 nuclear interactions to promote resistance to androgen receptor-targeting therapies.

Applications

Unspecified application

Species

Unspecified reactive species

Justin H Hwang,Rand Arafeh,Ji-Heui Seo,Sylvan C Baca,Megan Ludwig,Taylor E Arnoff,Lydia Sawyer,Camden Richter,Sydney Tape,Hannah E Bergom,Sean McSweeney,Jonathan P Rennhack,Sarah A Klingenberg,Alexander T M Cheung,Jason Kwon,Jonathan So,Steven Kregel,Eliezer M Van Allen,Justin M Drake,Matthew L Freedman,William C Hahn

Stem cells (Dayton, Ohio) 39:1322-1334 PubMed34058047

2021

Zbtb46-dependent altered developmental program in embryonic stem cell-derived blood cell progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Pal Boto,Timea Beatrix Gerzsenyi,Adel Lengyel,Balint Szunyog,Istvan Szatmari
View all publications
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