Rabbit Polyclonal IRF8 antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Irf8 aa 350 to C-terminus.
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Publications
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- Stem cells (Dayton, Ohio) 39:1322-13342021Zbtb46-dependent altered developmental program in embryonic stem cell-derived blood cell progenitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPal Boto,Timea Beatrix Gerzsenyi,Adel Lengyel,Balint Szunyog,Istvan SzatmariPubMed 34058047
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Irf8 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P23611
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Mouse | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) (PubMed:12393690, PubMed:2111015). Can both act as a transcriptional activator or repressor (PubMed:2111015). Plays a negative regulatory role in cells of the immune system (PubMed:2111015). Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (PubMed:12393690, PubMed:22992524). Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection (PubMed:12393690, PubMed:12461077, PubMed:12538667, PubMed:23382217). Positively regulates macroautophagy in dendritic cells (By similarity). Acts as a transcriptional repressor of osteoclast differentiation factors such as NFATC1 and EEIG1 (PubMed:32741026).
Alternative names
Icsbp, Icsbp1, Irf8, Interferon regulatory factor 8, IRF-8, Interferon consensus sequence-binding protein, ICSBP
Recommended products
Rabbit Polyclonal IRF8 antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Irf8 aa 350 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Irf8 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P23611
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab245607 was affinity purified using an epitope specific to IRF8 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IRF8 also known as Interferon Regulatory Factor 8 plays a significant role as a transcription factor in the regulation of gene expression. With a molecular mass of approximately 48 kDa this nuclear protein primarily functions in immune cells such as monocytes dendritic cells and macrophages. It binds to specific DNA sequences in gene promoters influencing the transcription of genes involved in immune response and development of blood cells. The expression of IRF8 extends beyond immune cells to tissues like the spleen and thymus.
- Biological function summary
IRF8 drives differentiation and maturation of various immune cell types. It forms part of protein complexes that control the expression of genes necessary for immune surveillance and defense mechanisms. IRF8 can dimerize with other IRF family members or proteins like PU.1 to modulate activity of specific target genes. This transcription factor supports the growth and differentiation of myeloid cells and helps to balance lymphoid and myeloid lineage commitment which is important for maintaining a proper immune system function.
- Pathways
IRF8 is integral to the interferon signaling and hematopoietic cell lineage pathways. It enhances the expression of interferon-stimulated genes integrating signals for a potent antiviral response. The protein connects with transcriptional regulators such as IRF1 and STAT1 to modulate these pathways. By participating in hematopoietic differentiation IRF8 also influences pathways related to the immune system development and inflammatory responses.
- Associated diseases and disorders
Mutations or alterations in IRF8 function associate with immunodeficiencies and hematologic malignancies. Chronic myelogenous leukemia and systemic lupus erythematosus exemplify conditions where IRF8 plays a role. Its dysfunction affects the regulation of cytokine genes which can lead to inappropriate immune responses. The protein's activity intersects with other key players such as BTK and BCL2 which are involved in disease processes related to immune cell proliferation and survival.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-IRF8 antibody (ab245607)
Prepared using NETN lysis buffer.
All lanes: Western blot - Anti-IRF8 antibody (ab245607) at 0.1 µg/mL
All lanes: L1210 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 48 kDa
Exposure time: 30s
Western blot - Anti-IRF8 antibody (ab245607)
Prepared using NETN lysis buffer.
All lanes: Western blot - Anti-IRF8 antibody (ab245607) at 0.1 µg/mL
Lane 1: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 50 µg
Lane 2: RAW 264.7 whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 48 kDa
Exposure time: 3min
Immunoprecipitation - Anti-IRF8 antibody (ab245607)
IRF8 was immunoprecipitated from RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245607 used for IP at 3 μg per reaction. For WB 1 μg/ml.Lane 1: ab245607 IP in RAW 264.7 whole cell lysate.
Lane 2: Control IgG IP in RAW 264.7 whole cell lysate.
Chemiluminescence detection: 3 minutes.All lanes: Immunoprecipitation - Anti-IRF8 antibody (ab245607)
Predicted band size: 48 kDa
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