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AB207418

Anti-IRF8 antibody [EPR20441]

5

(1 Review)

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(8 Publications)

Anti-IRF8 antibody [EPR20441] (ab207418) is a rabbit monoclonal antibody detecting IRF8 in Western Blot, IP, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

ICSBP1, IRF8, Interferon regulatory factor 8, IRF-8, Interferon consensus sequence-binding protein, H-ICSBP, ICSBP

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] (AB207418)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] (AB207418)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IRF8 with ab207418 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human tonsil (x200) [PMID : 18663414] [PMID : 16380510].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] (AB207418)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] (AB207418)

Immunohistochemical analysis of paraffin-embedded human non-hodgkin's lymphoma tissue labeling IRF8 with ab207418 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human non-hodgkin's lymphoma.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-IRF8 antibody [EPR20441] (AB207418)
  • IP

Supplier Data

Immunoprecipitation - Anti-IRF8 antibody [EPR20441] (AB207418)

IRF8 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab207418 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab207418 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Raji whole cell lysate 10 μg (Input).

Lane 2 : ab207418 IP in Raji whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab207418 in Raji whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-IRF8 antibody [EPR20441] (ab207418)

Predicted band size: 48 kDa

false

Western blot - Anti-IRF8 antibody [EPR20441] (AB207418)
  • WB

Supplier Data

Western blot - Anti-IRF8 antibody [EPR20441] (AB207418)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 30 seconds; Lane 2 : 2 seconds; Lane 3 : 15 seconds.

All lanes:

Western blot - Anti-IRF8 antibody [EPR20441] (ab207418) at 1/1000 dilution

Lane 1:

Human fetal spleen lysate at 10 µg

Lane 2:

Human tonsil lysate at 10 µg

Lane 3:

Human colon lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Western blot - Anti-IRF8 antibody [EPR20441] (AB207418)
  • WB

Supplier Data

Western blot - Anti-IRF8 antibody [EPR20441] (AB207418)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IRF8 antibody [EPR20441] (ab207418) at 1/1000 dilution

Lane 1:

THP-1 (Human monocytic leukemia cell line) whole cell lysate at 20 µg

Lane 2:

Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Exposure time: 1min

  • Carrier free

    Anti-IRF8 antibody [EPR20441] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20441

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-IRF8 antibody [EPR20441] (ab207418) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of IRF8?
Anti-IRF8 [EPR20441] (ab207418) specifically detects a band for IRF8 (UniProt: Q02556) at a molecular weight of 48kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR20441] also available for your convenience: ab207418, Carrier free - ab251475

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRF8 also known as Interferon Regulatory Factor 8 plays a significant role as a transcription factor in the regulation of gene expression. With a molecular mass of approximately 48 kDa this nuclear protein primarily functions in immune cells such as monocytes dendritic cells and macrophages. It binds to specific DNA sequences in gene promoters influencing the transcription of genes involved in immune response and development of blood cells. The expression of IRF8 extends beyond immune cells to tissues like the spleen and thymus.
Biological function summary

IRF8 drives differentiation and maturation of various immune cell types. It forms part of protein complexes that control the expression of genes necessary for immune surveillance and defense mechanisms. IRF8 can dimerize with other IRF family members or proteins like PU.1 to modulate activity of specific target genes. This transcription factor supports the growth and differentiation of myeloid cells and helps to balance lymphoid and myeloid lineage commitment which is important for maintaining a proper immune system function.

Pathways

IRF8 is integral to the interferon signaling and hematopoietic cell lineage pathways. It enhances the expression of interferon-stimulated genes integrating signals for a potent antiviral response. The protein connects with transcriptional regulators such as IRF1 and STAT1 to modulate these pathways. By participating in hematopoietic differentiation IRF8 also influences pathways related to the immune system development and inflammatory responses.

Mutations or alterations in IRF8 function associate with immunodeficiencies and hematologic malignancies. Chronic myelogenous leukemia and systemic lupus erythematosus exemplify conditions where IRF8 plays a role. Its dysfunction affects the regulation of cytokine genes which can lead to inappropriate immune responses. The protein's activity intersects with other key players such as BTK and BCL2 which are involved in disease processes related to immune cell proliferation and survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) (PubMed : 25122610). Can both act as a transcriptional activator or repressor (By similarity). Plays a negative regulatory role in cells of the immune system (By similarity). Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity). Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection (By similarity). Positively regulates macroautophagy in dendritic cells (PubMed : 29434592). Acts as a transcriptional repressor of osteoclast differentiation factors such as NFATC1 and EEIG1 (By similarity).
See full target information IRF8
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1048567 PubMed37205092

2023

Diffuse large B-cell lymphoma microenvironment displays a predominant macrophage infiltrate marked by a strong inflammatory signature.

Applications

Unspecified application

Species

Unspecified reactive species

Leyre Serna,Peio Azcoaga,Manisha Brahmachary,Maria M Caffarel,Mounia S Braza

iScience 25:105139 PubMed36193052

2022

Dissection of the MEF2D-IRF8 transcriptional circuit dependency in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Bianca Y Pingul,Hua Huang,Qingzhou Chen,Fatemeh Alikarami,Zhen Zhang,Jun Qi,Kathrin M Bernt,Shelley L Berger,Zhendong Cao,Junwei Shi

American journal of clinical pathology 158:173-176 PubMed35460405

2022

IRF8 as a Novel Marker to Differentiate Between CD30-Positive Large Cell Lymphomas.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel C McQuaid,Samuel G Katz,Mina L Xu

Human pathology 122:1-10 PubMed35085599

2022

Global assessment of IRF8 as a novel cancer biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel C McQuaid,Gauri Panse,Wei-Lien Wang,Geraldine S Pinkus,Samuel G Katz,Mina L Xu

Molecular cell 81:3604-3622.e10 PubMed34358447

2021

ZMYND8-regulated IRF8 transcription axis is an acute myeloid leukemia dependency.

Applications

Unspecified application

Species

Unspecified reactive species

Zhendong Cao,Krista A Budinich,Hua Huang,Diqiu Ren,Bin Lu,Zhen Zhang,Qingzhou Chen,Yeqiao Zhou,Yu-Han Huang,Fatemeh Alikarami,Molly C Kingsley,Alexandra K Lenard,Aoi Wakabayashi,Eugene Khandros,Will Bailis,Jun Qi,Martin P Carroll,Gerd A Blobel,Robert B Faryabi,Kathrin M Bernt,Shelley L Berger,Junwei Shi

Frontiers in immunology 12:674871 PubMed34290700

2021

Keratinocyte-Immune Cell Crosstalk in a STAT1-Mediated Pathway: Novel Insights Into Rosacea Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhili Deng,Fangfen Liu,Mengting Chen,Chuchu Huang,Wenqin Xiao,Sini Gao,Dan Jian,Yuyan Ouyang,San Xu,Jinmao Li,Qian Shi,Hongfu Xie,Guohong Zhang,Ji Li

Breast cancer research : BCR 23:40 PubMed33766090

2021

High IRF8 expression correlates with CD8 T cell infiltration and is a predictive biomarker of therapy response in ER-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gerardo Gatti,Courtney Betts,Darío Rocha,Maribel Nicola,Verónica Grupe,Cecilia Ditada,Nicolas G Nuñez,Emiliano Roselli,Paula Araya,Jeremías Dutto,Lucia Boffelli,Elmer Fernández,Lisa M Coussens,Mariana Maccioni

Nucleic acids research 47:e31 PubMed30657937

2019

NextPBM: a platform to study cell-specific transcription factor binding and cooperativity.

Applications

Unspecified application

Species

Unspecified reactive species

Nima Mohaghegh,David Bray,Jessica Keenan,Ashley Penvose,Kellen K Andrilenas,Vijendra Ramlall,Trevor Siggers
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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