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Rabbit Recombinant Monoclonal IRF8 antibody. Carrier free. Suitable for WB, IP, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Mouse, Rat samples.

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Images

Flow Cytometry (Intracellular) - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (AB307293), expandable thumbnail
  • Western blot - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (AB307293), expandable thumbnail
  • Immunoprecipitation - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (AB307293), expandable thumbnail
  • Western blot - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (AB307293), expandable thumbnail
  • Western blot - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (AB307293), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPFlow Cyt (Intra)ICC/IFIHC-FrIHC-P
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Tested
Not recommended
Tested
Rat
Tested
Expected
Expected
Expected
Not recommended
Tested

Tested
Tested

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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    Target data

    Function

    Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) (PubMed:2111015, PubMed:12393690). Can both act as a transcriptional activator or repressor (PubMed:2111015). Plays a negative regulatory role in cells of the immune system (PubMed:2111015). Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (PubMed:12393690, PubMed:22992524). Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection (PubMed:12461077, PubMed:12393690, PubMed:12538667, PubMed:23382217). Positively regulates macroautophagy in dendritic cells (By similarity).

    Alternative names

    Interferon regulatory factor 8, IRF-8, Interferon consensus sequence-binding protein, ICSBP, Icsbp, Icsbp1, Irf8

    Recommended products

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    Rabbit Recombinant Monoclonal IRF8 antibody. Carrier free. Suitable for WB, IP, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Mouse, Rat samples.

    Alternative names

    Interferon regulatory factor 8, IRF-8, Interferon consensus sequence-binding protein, ICSBP, Icsbp, Icsbp1, Irf8

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR26381-181

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Supplementary info

    Activity summary

    IRF8 also known as Interferon Regulatory Factor 8 plays a significant role as a transcription factor in the regulation of gene expression. With a molecular mass of approximately 48 kDa this nuclear protein primarily functions in immune cells such as monocytes dendritic cells and macrophages. It binds to specific DNA sequences in gene promoters influencing the transcription of genes involved in immune response and development of blood cells. The expression of IRF8 extends beyond immune cells to tissues like the spleen and thymus.

    Biological function summary

    IRF8 drives differentiation and maturation of various immune cell types. It forms part of protein complexes that control the expression of genes necessary for immune surveillance and defense mechanisms. IRF8 can dimerize with other IRF family members or proteins like PU.1 to modulate activity of specific target genes. This transcription factor supports the growth and differentiation of myeloid cells and helps to balance lymphoid and myeloid lineage commitment which is important for maintaining a proper immune system function.

    Pathways

    IRF8 is integral to the interferon signaling and hematopoietic cell lineage pathways. It enhances the expression of interferon-stimulated genes integrating signals for a potent antiviral response. The protein connects with transcriptional regulators such as IRF1 and STAT1 to modulate these pathways. By participating in hematopoietic differentiation IRF8 also influences pathways related to the immune system development and inflammatory responses.

    Associated diseases and disorders

    Mutations or alterations in IRF8 function associate with immunodeficiencies and hematologic malignancies. Chronic myelogenous leukemia and systemic lupus erythematosus exemplify conditions where IRF8 plays a role. Its dysfunction affects the regulation of cytokine genes which can lead to inappropriate immune responses. The protein's activity intersects with other key players such as BTK and BCL2 which are involved in disease processes related to immune cell proliferation and survival.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    11 product images

    • Flow Cytometry (Intracellular) - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293), expandable thumbnail

      Flow Cytometry (Intracellular) - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293)

      This data was developed using Anti-IRF8 antibody [EPR26381-181] ab307292, the same antibody clone in a different buffer formulation.
      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A20 (mouse reticulum sarcoma B lymphocyte) cells labeling IRF8 with Anti-IRF8 antibody [EPR26381-181] ab307292 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

    • Western blot - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293), expandable thumbnail

      Western blot - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293)

      This data was developed using Anti-IRF8 antibody [EPR26381-181] ab307292, the same antibody clone in a different buffer formulation.
      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative and positive control (PMID: 2111015).

      Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

      This blot was developed using a high sensitivity ECL substrate.

      Exposure time: 180 seconds.

      All lanes: Western blot - Anti-IRF8 antibody [EPR26381-181] (Anti-IRF8 antibody [EPR26381-181] ab307292) at 1/1000 dilution

      Lane 1: Rat brain tissue lysate 20 μg

      Lane 2: Rat spleen tissue lysate 20 μg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Observed band size: 48 kDa

      Exposure time: 180s

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative and positive control (PMID: 2111015).

      Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

      This blot was developed using a high sensitivity ECL substrate.

      Exposure time: 180 seconds.

    • Immunoprecipitation - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293), expandable thumbnail

      Immunoprecipitation - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293)

      This data was developed using Anti-IRF8 antibody [EPR26381-181] ab307292, the same antibody clone in a different buffer formulation.
      IRF8 was immunoprecipitated from 0.35 mg A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate 10 ug with Anti-IRF8 antibody [EPR26381-181] ab307292 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-IRF8 antibody [EPR26381-181] ab307292 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

      Lane 1: A20 whole cell lysate 10 ug

      Lane 2: Anti-IRF8 antibody [EPR26381-181] ab307292 IP in A20 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IRF8 antibody [EPR26381-181] ab307292 in A20 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 76 seconds.

      All lanes: Immunoprecipitation - Anti-IRF8 antibody [EPR26381-181] (Anti-IRF8 antibody [EPR26381-181] ab307292) at 1/1000 dilution

      Lane 1: A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate 10 μg

      Lane 2: A20 whole cell lysate

      Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

      Secondary

      All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

      Observed band size: 48 kDa

      Exposure time: 76s

      IRF8 was immunoprecipitated from 0.35 mg A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate 10 ug with Anti-IRF8 antibody [EPR26381-181] ab307292 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-IRF8 antibody [EPR26381-181] ab307292 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

      Lane 1: A20 whole cell lysate 10 ug

      Lane 2: Anti-IRF8 antibody [EPR26381-181] ab307292 IP in A20 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IRF8 antibody [EPR26381-181] ab307292 in A20 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 76 seconds.

    • Western blot - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293), expandable thumbnail

      Western blot - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293)

      This data was developed using Anti-IRF8 antibody [EPR26381-181] ab307292, the same antibody clone in a different buffer formulation.
      Blocking/dilution buffer: 5% NFDM/TBST.

      Negative and positive control (PMID: 2111015).
      Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

      This blot was developed using a high sensitivity ECL substrate.

      Exposure time: 180 seconds.

      All lanes: Western blot - Anti-IRF8 antibody [EPR26381-181] (Anti-IRF8 antibody [EPR26381-181] ab307292) at 1/1000 dilution

      Lane 1: Mouse brain tissue lysate 20 μg

      Lane 2: Mouse spleen tissue lysate 20 μg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Observed band size: 48 kDa

      Exposure time: 180s

      Blocking/dilution buffer: 5% NFDM/TBST.

      Negative and positive control (PMID: 2111015).
      Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

      This blot was developed using a high sensitivity ECL substrate.

      Exposure time: 180 seconds.

    • Western blot - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293), expandable thumbnail

      Western blot - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293)

      This data was developed using Anti-IRF8 antibody [EPR26381-181] ab307292, the same antibody clone in a different buffer formulation.
      Blocking and diluting buffer and concentration: 5% NFDM/TBST  

      Exposure time: 92 seconds

      All lanes: Western blot - Anti-IRF8 antibody [EPR26381-181] (Anti-IRF8 antibody [EPR26381-181] ab307292) at 1/1000 dilution

      All lanes: A20 (mouse reticum sarcoma B lymphocyte) whole cell lysate 20μg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Observed band size: 48 kDa

      Exposure time: 92s

      Blocking and diluting buffer and concentration: 5% NFDM/TBST  

      Exposure time: 92 seconds

    • Immunohistochemistry - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293), expandable thumbnail

      Immunohistochemistry - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293)

      This data was developed using Anti-IRF8 antibody [EPR26381-181] ab307292, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling IRF8 with Anti-IRF8 antibody [EPR26381-181] ab307292 at 1/500 (1.06 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Negative control: almost no staining on rat cerebrum.

      The section was incubated with Anti-IRF8 antibody [EPR26381-181] ab307292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunocytochemistry/ Immunofluorescence - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293), expandable thumbnail

      Immunocytochemistry/ Immunofluorescence - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293)

      This data was developed using Anti-IRF8 antibody [EPR26381-181] ab307292, the same antibody clone in a different buffer formulation.
      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A20 (mouse reticulum sarcoma B lymphocyte) cells labeling IRF8 with Anti-IRF8 antibody [EPR26381-181] ab307292 at 1/100 (5.3 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

      Confocal image showing mainly nuclear staining in A20 cell line.

      Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

    • Immunohistochemistry - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293), expandable thumbnail

      Immunohistochemistry - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293)

      This data was developed using Anti-IRF8 antibody [EPR26381-181] ab307292, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling IRF8 with Anti-IRF8 antibody [EPR26381-181] ab307292 at 1/500 (1.06 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat spleen. The section was incubated with Anti-IRF8 antibody [EPR26381-181] ab307292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293), expandable thumbnail

      Immunohistochemistry - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293)

      This data was developed using Anti-IRF8 antibody [EPR26381-181] ab307292, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling IRF8 with Anti-IRF8 antibody [EPR26381-181] ab307292 at 1/500 (1.06 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Negative control: almost no staining on mouse cerebrum.

      The section was incubated with Anti-IRF8 antibody [EPR26381-181] ab307292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293), expandable thumbnail

      Immunohistochemistry - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293)

      This data was developed using Anti-IRF8 antibody [EPR26381-181] ab307292, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling IRF8 with Anti-IRF8 antibody [EPR26381-181] ab307292 at 1/500 (1.06 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse spleen. The section was incubated with Anti-IRF8 antibody [EPR26381-181] ab307292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293), expandable thumbnail

      Immunohistochemistry - Anti-IRF8 antibody [EPR26381-181] - BSA and Azide free (ab307293)

      This data was developed using Anti-IRF8 antibody [EPR26381-181] ab307292, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded mouse Burkitt's lymphoma tissue labeling IRF8 with Anti-IRF8 antibody [EPR26381-181] ab307292 at 1/500 (1.06 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse Burkitt's lymphoma. The section was incubated with Anti-IRF8 antibody [EPR26381-181] ab307292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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