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AB313472

Anti-IRF8 antibody [RP23040181]

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Rabbit Recombinant Multiclonal IRF8 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human IRF8.

View Alternative Names

ICSBP1, IRF8, Interferon regulatory factor 8, IRF-8, Interferon consensus sequence-binding protein, H-ICSBP, ICSBP

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-IRF8 Antibody [RP23040181] (AB313472)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IRF8 Antibody [RP23040181] (AB313472)

For immunofluorescence analysis, IFN gamma (300 IU, 6 h) treated THP1 cells were fixed and permeabilized for detection of endogenous IRF8 using ab313472 (2 ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate ( 1 : 2000). Panel a) shows representative cells that were stained for detection and localization of IRF8 protein (green), Panel b) is stained for nuclei (blue) using with DAPI . Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin ( 1 : 300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of IRF8. A lower signal intensity was observed in untreated cells, represented in Panel e). Panel f) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040181

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human IRF8. Database link Q02556

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRF8 also known as Interferon Regulatory Factor 8 plays a significant role as a transcription factor in the regulation of gene expression. With a molecular mass of approximately 48 kDa this nuclear protein primarily functions in immune cells such as monocytes dendritic cells and macrophages. It binds to specific DNA sequences in gene promoters influencing the transcription of genes involved in immune response and development of blood cells. The expression of IRF8 extends beyond immune cells to tissues like the spleen and thymus.
Biological function summary

IRF8 drives differentiation and maturation of various immune cell types. It forms part of protein complexes that control the expression of genes necessary for immune surveillance and defense mechanisms. IRF8 can dimerize with other IRF family members or proteins like PU.1 to modulate activity of specific target genes. This transcription factor supports the growth and differentiation of myeloid cells and helps to balance lymphoid and myeloid lineage commitment which is important for maintaining a proper immune system function.

Pathways

IRF8 is integral to the interferon signaling and hematopoietic cell lineage pathways. It enhances the expression of interferon-stimulated genes integrating signals for a potent antiviral response. The protein connects with transcriptional regulators such as IRF1 and STAT1 to modulate these pathways. By participating in hematopoietic differentiation IRF8 also influences pathways related to the immune system development and inflammatory responses.

Mutations or alterations in IRF8 function associate with immunodeficiencies and hematologic malignancies. Chronic myelogenous leukemia and systemic lupus erythematosus exemplify conditions where IRF8 plays a role. Its dysfunction affects the regulation of cytokine genes which can lead to inappropriate immune responses. The protein's activity intersects with other key players such as BTK and BCL2 which are involved in disease processes related to immune cell proliferation and survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) (PubMed : 25122610). Can both act as a transcriptional activator or repressor (By similarity). Plays a negative regulatory role in cells of the immune system (By similarity). Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity). Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection (By similarity). Positively regulates macroautophagy in dendritic cells (PubMed : 29434592). Acts as a transcriptional repressor of osteoclast differentiation factors such as NFATC1 and EEIG1 (By similarity).
See full target information IRF8
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Product promise

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