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AB222417

Anti-IRG1 antibody

3

(2 Reviews)

|

(2 Publications)

Rabbit Polyclonal IRG1 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

IRG1, ACOD1, Cis-aconitate decarboxylase, CAD, Aconitate decarboxylase, Aconitate decarboxylase 1, Cis-aconitic acid decarboxylase, Immune-responsive gene 1 protein

1 Images
Western blot - Anti-IRG1 antibody (AB222417)
  • WB

Supplier Data

Western blot - Anti-IRG1 antibody (AB222417)

All lanes:

Western blot - Anti-IRG1 antibody (ab222417) at 1/400 dilution

Lane 1:

Mouse heart tissue lysate at 40 µg

Lane 2:

K562 cell lysate at 40 µg

Secondary

All lanes:

Goat anti rabbit IgG secondary antibody at 1/8000 dilution

Predicted band size: 53 kDa

false

Exposure time: 20s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRG1 also known as Immune-Responsive Gene 1 protein has a molecular weight of approximately 50 kDa. This protein is expressed largely in immune cells such as macrophages and dendritic cells. Mechanically IRG1 catalyzes the production of itaconate from cis-aconitate during the citric acid cycle an action that has gained significant interest due to its immunomodulatory effects. The localization of IRG1 in immune cells suggests a role in the response to pathogenic threats and regulation of inflammation.
Biological function summary

IRG1 is important for immune response regulation by producing itaconate an anti-inflammatory metabolite. Itaconate acts on innate immune signaling pathways to dampen excessive inflammatory responses. Though IRG1 is not part of a larger protein complex it interacts closely with cellular metabolism. This interaction plays a significant role in metabolic reprogramming during infections thereby influencing cytokine production and the activation state of cells.

Pathways

IRG1 plays a significant role in the tricarboxylic acid (TCA) cycle and is associated with the innate immune response. The protein shifts cellular metabolism towards cataplerosis to accommodate the need for itaconate production. IRG1 is connected to regulatory proteins such as the transcription factor NRF2 which mediates antioxidative responses. Itaconate a product of IRG1 activity can modulate pathways like the NF-kB signaling pathway attenuating inflammatory responses.

IRG1 has been implicated in atherosclerosis and tuberculosis. In atherosclerosis IRG1 contributes to the anti-inflammatory state within macrophages which can alter plaque formation. Through itaconate IRG1 interacts with inflammasome components potentially impacting the pathology of the disease. In tuberculosis IRG1-induced itaconate production helps in macrophage defense against Mycobacterium tuberculosis. Additionally pathways involving IRG1 often intersect with proteins like HIF-1α under hypoxic conditions relevant to the progression and management of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cis-aconitate decarboxylase that catalyzes production of itaconate and is involved in the inhibition of the inflammatory response (PubMed : 23609450, PubMed : 23610393, PubMed : 31548418, PubMed : 35662396). Acts as a negative regulator of the Toll-like receptors (TLRs)-mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages (PubMed : 23609450). Involved in antimicrobial response of innate immune cells; ACOD1-mediated itaconic acid production contributes to the antimicrobial activity of macrophages by generating itaconate, leading to alkylation of proteins, such as TFEB (PubMed : 23610393, PubMed : 35662396). Involved in antiviral response following infection by flavivirus in neurons : ACOD1-mediated itaconate production inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes (By similarity). Plays a role in the embryo implantation (By similarity).
See full target information ACOD1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature chemical biology 21:360-370 PubMed39223388

2024

An engineered trafficking biosensor reveals a role for DNAJC13 in DOR downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon Novy,Aleksandra Dagunts,Tatum Weishaar,Emily E Holland,Hayden Adoff,Emily Hutchinson,Monica De Maria,Martin Kampmann,Nikoleta G Tsvetanova,Braden T Lobingier

mBio 10: PubMed31719183

2019

IRG1 and Inducible Nitric Oxide Synthase Act Redundantly with Other Interferon-Gamma-Induced Factors To Restrict Intracellular Replication of Legionella pneumophila.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan V Price,Daniel Russo,Daisy X Ji,Roberto A Chavez,Lucian DiPeso,Angus Yiu-Fai Lee,Jörn Coers,Russell E Vance
View all publications
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Product promise

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