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AB238580

Anti-IRG1 antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal IRG1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACOD1 aa 50-300.

View Alternative Names

IRG1, ACOD1, Cis-aconitate decarboxylase, CAD, Aconitate decarboxylase, Aconitate decarboxylase 1, Cis-aconitic acid decarboxylase, Immune-responsive gene 1 protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRG1 antibody (AB238580)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRG1 antibody (AB238580)

Paraffin-embedded human appendix tissue stained for IRG1 using ab238580 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRG1 antibody (AB238580)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRG1 antibody (AB238580)

Paraffin-embedded human glioma tissue stained for IRG1 using ab238580 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ACOD1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

A6NK06

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Alternative Products

Primary Antibodies

AB222411

Anti-IRG1 antibody [EPR22066]

RabMAb|Recombinant

Western blot - Anti-IRG1 antibody [EPR22066] (AB222411)

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Primary Antibodies

AB238169

Anti-IRG1 antibody [EPR22066] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-IRG1 antibody [EPR22066] - BSA and Azide free (AB238169)

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Primary Antibodies

AB222417

Anti-IRG1 antibody

Western blot - Anti-IRG1 antibody (AB222417)

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Complementary Products

Assay Kits

AB83459

Aconitase Assay Kit

Functional Studies - Aconitase Assay Kit (AB83459)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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  • 20
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

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  • 105
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

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Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 49
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRG1 also known as Immune-Responsive Gene 1 protein has a molecular weight of approximately 50 kDa. This protein is expressed largely in immune cells such as macrophages and dendritic cells. Mechanically IRG1 catalyzes the production of itaconate from cis-aconitate during the citric acid cycle an action that has gained significant interest due to its immunomodulatory effects. The localization of IRG1 in immune cells suggests a role in the response to pathogenic threats and regulation of inflammation.
Biological function summary

IRG1 is important for immune response regulation by producing itaconate an anti-inflammatory metabolite. Itaconate acts on innate immune signaling pathways to dampen excessive inflammatory responses. Though IRG1 is not part of a larger protein complex it interacts closely with cellular metabolism. This interaction plays a significant role in metabolic reprogramming during infections thereby influencing cytokine production and the activation state of cells.

Pathways

IRG1 plays a significant role in the tricarboxylic acid (TCA) cycle and is associated with the innate immune response. The protein shifts cellular metabolism towards cataplerosis to accommodate the need for itaconate production. IRG1 is connected to regulatory proteins such as the transcription factor NRF2 which mediates antioxidative responses. Itaconate a product of IRG1 activity can modulate pathways like the NF-kB signaling pathway attenuating inflammatory responses.

IRG1 has been implicated in atherosclerosis and tuberculosis. In atherosclerosis IRG1 contributes to the anti-inflammatory state within macrophages which can alter plaque formation. Through itaconate IRG1 interacts with inflammasome components potentially impacting the pathology of the disease. In tuberculosis IRG1-induced itaconate production helps in macrophage defense against Mycobacterium tuberculosis. Additionally pathways involving IRG1 often intersect with proteins like HIF-1α under hypoxic conditions relevant to the progression and management of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cis-aconitate decarboxylase that catalyzes production of itaconate and is involved in the inhibition of the inflammatory response (PubMed : 23609450, PubMed : 23610393, PubMed : 31548418, PubMed : 35662396). Acts as a negative regulator of the Toll-like receptors (TLRs)-mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages (PubMed : 23609450). Involved in antimicrobial response of innate immune cells; ACOD1-mediated itaconic acid production contributes to the antimicrobial activity of macrophages by generating itaconate, leading to alkylation of proteins, such as TFEB (PubMed : 23610393, PubMed : 35662396). Involved in antiviral response following infection by flavivirus in neurons : ACOD1-mediated itaconate production inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes (By similarity). Plays a role in the embryo implantation (By similarity).
See full target information ACOD1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Radiology and oncology 58:67-77 PubMed38378037

2024

RAD54B promotes gastric cancer cell migration and angiogenesis via the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianchao Li,Hui Geng,Xin Li,Shenshan Zou,Xintao Xu

The Journal of clinical investigation 134: PubMed38085578

2023

Itaconate suppresses atherosclerosis by activating a Nrf2-dependent antiinflammatory response in macrophages in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jianrui Song,Yanling Zhang,Ryan A Frieler,Anthony Andren,Sherri Wood,Daniel J Tyrrell,Peter Sajjakulnukit,Jane C Deng,Costas A Lyssiotis,Richard M Mortensen,Morgan Salmon,Daniel R Goldstein
View all publications
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Product promise

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