Skip to main content

Rabbit Recombinant Monoclonal IRR antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Immunoprecipitation - Anti-IRR antibody [EPR24776-127] - BSA and Azide free (AB282736), expandable thumbnail
  • Western blot - Anti-IRR antibody [EPR24776-127] - BSA and Azide free (AB282736), expandable thumbnail
  • Western blot - Anti-IRR antibody [EPR24776-127] - BSA and Azide free (AB282736), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Tested
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Receptor with tyrosine-protein kinase activity. Functions as a pH sensing receptor which is activated by increased extracellular pH. Activates an intracellular signaling pathway that involves IRS1 and AKT1/PKB.

Alternative names

IRR, INSRR, Insulin receptor-related protein, IR-related receptor

Recommended products

Rabbit Recombinant Monoclonal IRR antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR24776-127
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab282736 is the carrier-free version of Anti-IRR antibody [EPR24776-127] ab273125.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Insulin Receptor-Related Receptor (IRR) also called INSRR is a receptor tyrosine kinase that is linked structurally to the insulin receptor. This protein has a molecular mass of about 140 kDa. IRR expresses in tissues such as the kidney lung and stomach. It functions mainly as a pH sensor playing a role in regulating cellular responses to extracellular pH changes. Through its intrinsic kinase activity IRR phosphorylates specific substrates upon ligand binding initiating downstream signaling events.

Biological function summary

IRR acts in a manner analogous to other receptor tyrosine kinases but is unique in its role as a pH sensor impacting various physiological processes. It does not usually form part of larger protein complexes but influences cellular homeostasis and adaptive responses to acidic conditions. Its activity may affect cellular growth and differentiation. Researchers have identified that IRR contributes to maintaining the pH balance in tissues where it is present which implies its importance in controlling basic cellular functions.

Pathways

The IRR participates in signaling pathways that monitor and regulate pH homeostasis and related metabolic responses. It connects with the MAPK/ERK pathway which influences cell proliferation and stress responses. This connection links IRR and MAPK/ERK to processes essential for cellular adaptation to changing pH conditions. The presence of proteins like Akt which also has roles in the pathways involving insulin receptor signals suggests IRR might have related but distinct signaling functions.

Associated diseases and disorders

The understanding of IRR's role relates to conditions such as cancer and metabolic acidosis. Changes in its activity may influence cancer progression due to its ties with cell proliferation pathways. Abnormal IRR signaling potentially impacts acidosis-related metabolic dysfunctions where IRR's regulatory action on pH becomes critical. In these contexts proteins like the insulin receptor may share overlapping functionalities with IRR as both are receptor tyrosine kinases although IRR’s specific disease links remain less characterized.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunoprecipitation - Anti-IRR antibody [EPR24776-127] - BSA and Azide free (ab282736), expandable thumbnail

    Immunoprecipitation - Anti-IRR antibody [EPR24776-127] - BSA and Azide free (ab282736)

    This data was developed using Anti-IRR antibody [EPR24776-127] ab273125, the same antibody clone in a different buffer formulation.

    IRR was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with Anti-IRR antibody [EPR24776-127] ab273125 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-IRR antibody [EPR24776-127] ab273125 at 1/1000 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used at 1/5000 dilution.

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 μg

    Lane 2: Anti-IRR antibody [EPR24776-127] ab273125 IP in HepG2 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IRR antibody [EPR24776-127] ab273125 in HepG2 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

    All lanes: Immunoprecipitation - Anti-IRR antibody [EPR24776-127] (Anti-IRR antibody [EPR24776-127] ab273125)

    Predicted band size: 144 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-IRR antibody [EPR24776-127] - BSA and Azide free (ab282736), expandable thumbnail

    Western blot - Anti-IRR antibody [EPR24776-127] - BSA and Azide free (ab282736)

    This data was developed using Anti-IRR antibody [EPR24776-127] ab273125, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration:5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29156593).

    Negative control: U-937.

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-IRR antibody [EPR24776-127] (Anti-IRR antibody [EPR24776-127] ab273125) at 1/1000 dilution

    Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 144 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-IRR antibody [EPR24776-127] - BSA and Azide free (ab282736), expandable thumbnail

    Western blot - Anti-IRR antibody [EPR24776-127] - BSA and Azide free (ab282736)

    This data was developed using Anti-IRR antibody [EPR24776-127] ab273125, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-IRR antibody [EPR24776-127] (Anti-IRR antibody [EPR24776-127] ab273125) at 1/5000 dilution

    All lanes: Human heart tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 144 kDa

    Observed band size: 60 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com