JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB131509

Anti-IRS1 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal IRS1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Insulin receptor substrate 1 aa 600-650.

View Alternative Names

Insulin receptor substrate 1, IRS-1, IRS1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-IRS1 antibody (AB131509)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IRS1 antibody (AB131509)

Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131509 at 1/100 dilution.

Western blot - Anti-IRS1 antibody (AB131509)
  • WB

Unknown

Western blot - Anti-IRS1 antibody (AB131509)

All lanes:

Western blot - Anti-IRS1 antibody (ab131509) at 1/500 dilution

Lane 1:

HT29 cell extract

Lane 2:

3T3 cell extract

Predicted band size: 131 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human Insulin receptor substrate 1 aa 600-650. The exact immunogen used to generate this antibody is proprietary information.

P35568

style
left-column

Alternative Products

Primary Antibodies

AB131487

Anti-IRS1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody (AB131487)

  • 5
  • 3
Primary Antibodies

AB5599

Anti-IRS1 (phospho S307) antibody

Immunocytochemistry - Anti-IRS1 (phospho S307) antibody (AB5599)

  • 0
  • 0
Primary Antibodies

AB109543

Anti-IRS1 (phospho Y632) antibody [EPR259(2)]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-IRS1 (phospho Y632) antibody [EPR259(2)] (AB109543)

  • 0
  • 0
Primary Antibodies

AB46800

Anti-IRS1 antibody [EP260Y]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-IRS1 antibody [EP260Y] (AB46800)

  • 0
  • 0
Primary Antibodies

AB40777

Anti-IRS1+IRS2 antibody [EP263Y]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777)

  • 0
  • 0
Primary Antibodies

AB232463

Anti-IRS1 antibody [EP260Y] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-IRS1 antibody [EP260Y] - BSA and Azide free (AB232463)

  • 0
  • 0

Complementary Products

Assay Kits

AB136955

Glucose Uptake Assay Kit (Colorimetric)

Functional Studies - Glucose Uptake Assay Kit (Colorimetric) (AB136955)

  • 5
  • 1
Primary Antibodies

AB134101

Anti-IRS2 antibody [EPR904(2)]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS2 antibody [EPR904(2)] (AB134101)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB33780

Anti-Glucose Transporter GLUT4 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (AB33780)

  • 4
  • 12
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein IRS1 also known as Insulin Receptor Substrate 1 is an important player in insulin signaling. It is a docking protein that plays a part in the signaling pathway of insulin and insulin-like growth factor (IGF) contributing to glucose homeostasis. The molecular weight of IRS1 is approximately 180 kDa. Expressed in various tissues including muscle liver and adipose tissue IRS1 facilitates the transmission of signals from activated cell surface receptors to intracellular pathways. Notable for its phosphorylation IRS1 undergoes change in state when interacting with receptor kinases impacting its role in cell signaling.
Biological function summary

IRS1 helps mediate the effects of insulin by acting as a downstream effector in the insulin signaling cascade. IRS1 functions in a complex manner binding with PI3K (phosphoinositide 3-kinase) after phosphorylation at specific tyrosine residues. This interaction is important for further signaling events leading to the regulation of glucose uptake lipid synthesis and gene expression. Besides its role in energy balance IRS1 plays a significant part in cell growth and differentiation driven by its ability to relay signals from the insulin and IGF receptors.

Pathways

IRS1 is heavily involved in the insulin signaling pathway and the PI3K-Akt pathway. Upon phosphorylation IRS1 binds to proteins such as the PI3K initiating a cascade that ultimately activates Akt resulting in anabolic processes within cells. The pathway is essential for regulating basic functions like metabolism growth and survival. IRS1's interaction with other related proteins like IR (insulin receptor) and IGFR (insulin-like growth factor receptor) highlights its integrative role in cellular processes related to energy and growth.

IRS1 is implicated in insulin resistance and type 2 diabetes. Changes in IRS1 phosphorylation patterns can disrupt normal insulin signaling leading to impaired glucose uptake and metabolic dysregulation. The protein's dysfunction is linked to obesity forming a connection through its interactions with other proteins like Akt and mTOR. Aberrant IRS1 activity may contribute to altered cell metabolism and growth promoting conditions like diabetes which highlights the importance of IRS1 in maintaining metabolic health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R (PubMed : 7541045, PubMed : 33991522, PubMed : 38625937). Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism (PubMed : 19639489). Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2 (PubMed : 11171109, PubMed : 8265614). Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade (By similarity). Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. Acts a positive regulator of the Wnt/beta-catenin signaling pathway through suppression of DVL2 autophagy-mediated degradation leading to cell proliferation (PubMed : 24616100).
See full target information Insulin receptor substrate 1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 18:1366098 PubMed38644975

2024

Micro-and mesoscale aspects of neurodegeneration in engineered human neural networks carrying the LRRK2 G2019S mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Vibeke Devold Valderhaug,Ola Huse Ramstad,Rosanne van de Wijdeven,Kristine Heiney,Stefano Nichele,Axel Sandvig,Ioanna Sandvig
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com