AB5603

Anti-IRS1 antibody

Name: Anti-IRS1 antibody (ab5603) | Abcam
Brand: Abcam Limited
SKU: AB5603
Price: 525 USD
Availability: InStock

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(42 Publications)

Rabbit Polyclonal IRS1 antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 42 publications. Immunogen corresponding to Synthetic Peptide within Human Insulin receptor substrate 1 aa 600-650.

View Alternative Names

Insulin receptor substrate 1, IRS-1, IRS1

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human Insulin receptor substrate 1 aa 600-650. The exact immunogen used to generate this antibody is proprietary information.

P35568

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein IRS1 also known as Insulin Receptor Substrate 1 is an important player in insulin signaling. It is a docking protein that plays a part in the signaling pathway of insulin and insulin-like growth factor (IGF) contributing to glucose homeostasis. The molecular weight of IRS1 is approximately 180 kDa. Expressed in various tissues including muscle liver and adipose tissue IRS1 facilitates the transmission of signals from activated cell surface receptors to intracellular pathways. Notable for its phosphorylation IRS1 undergoes change in state when interacting with receptor kinases impacting its role in cell signaling.

Biological function summary

IRS1 helps mediate the effects of insulin by acting as a downstream effector in the insulin signaling cascade. IRS1 functions in a complex manner binding with PI3K (phosphoinositide 3-kinase) after phosphorylation at specific tyrosine residues. This interaction is important for further signaling events leading to the regulation of glucose uptake lipid synthesis and gene expression. Besides its role in energy balance IRS1 plays a significant part in cell growth and differentiation driven by its ability to relay signals from the insulin and IGF receptors.

Pathways

IRS1 is heavily involved in the insulin signaling pathway and the PI3K-Akt pathway. Upon phosphorylation IRS1 binds to proteins such as the PI3K initiating a cascade that ultimately activates Akt resulting in anabolic processes within cells. The pathway is essential for regulating basic functions like metabolism growth and survival. IRS1's interaction with other related proteins like IR (insulin receptor) and IGFR (insulin-like growth factor receptor) highlights its integrative role in cellular processes related to energy and growth.

IRS1 is implicated in insulin resistance and type 2 diabetes. Changes in IRS1 phosphorylation patterns can disrupt normal insulin signaling leading to impaired glucose uptake and metabolic dysregulation. The protein's dysfunction is linked to obesity forming a connection through its interactions with other proteins like Akt and mTOR. Aberrant IRS1 activity may contribute to altered cell metabolism and growth promoting conditions like diabetes which highlights the importance of IRS1 in maintaining metabolic health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R (PubMed : 7541045, PubMed : 33991522, PubMed : 38625937). Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism (PubMed : 19639489). Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2 (PubMed : 11171109, PubMed : 8265614). Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade (By similarity). Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. Acts a positive regulator of the Wnt/beta-catenin signaling pathway through suppression of DVL2 autophagy-mediated degradation leading to cell proliferation (PubMed : 24616100).

See full target information Insulin receptor substrate 1

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Publications (42)

Recent publications for all applications. Explore the full list and refine your search

Brain and behavior 14:e70110 PubMed39482855

2024

Correlation Between Regulation of Intestinal Flora by Danggui-Shaoyao-San and Improvement of Cognitive Impairment in Mice With Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Han Wang,Peng-Li Ding,Kai-Xin Zhang,Xiang-Qing Xu,He Li

Nature communications 14:5986 PubMed37794031

2023

Integration of 3D-printed cerebral cortical tissue into an ex vivo lesioned brain slice.

Applications

Unspecified application

Species

Unspecified reactive species

Yongcheng Jin,Ellina Mikhailova,Ming Lei,Sally A Cowley,Tianyi Sun,Xingyun Yang,Yujia Zhang,Kaili Liu,Daniel Catarino da Silva,Luana Campos Soares,Sara Bandiera,Francis G Szele,Zoltán Molnár,Linna Zhou,Hagan Bayley

Cell biology international 48:46-59 PubMed37750505

2023

Pachymic acid (PA) inhibits ferroptosis of cardiomyocytes via activation of the AMPK in mice with ischemia/reperfusion-induced myocardial injury.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmin Liu,Jiru Ding,Zhenzhen Li,Youquan Lu

Biology open 12: PubMed36504260

2023

Plpp3, a novel regulator of pluripotency exit and endodermal differentiation of mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Martha E Montané-Romero,Ana V Martínez-Silva,Augusto C Poot-Hernández,Diana Escalante-Alcalde

International journal of molecular sciences 23: PubMed35806005

2022

Human IL12p80 Promotes Murine Oligodendrocyte Differentiation to Repair Nerve Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Fen Chung,Jong-Hang Chen,Ching-Wen Li,Hui-Yu Hsu,Ya-Ping Chen,Chiao-Chan Wang,Ing-Ming Chiu

Scientific reports 11:21590 PubMed34732776

2021

Developmental defects and impaired network excitability in a cerebral organoid model of KCNJ11 p.V59M-related neonatal diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Gokhan Dalgin,Andrew K Tryba,Ashley P Cohen,Soo-Young Park,Louis H Philipson,Siri Atma W Greeley,Alfredo J Garcia

Cell stem cell 28:1549-1565.e12 PubMed33915080

2021

Principles of signaling pathway modulation for enhancing human naive pluripotency induction.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Bayerl,Muneef Ayyash,Tom Shani,Yair Shlomo Manor,Ohad Gafni,Rada Massarwa,Yael Kalma,Alejandro Aguilera-Castrejon,Mirie Zerbib,Hadar Amir,Daoud Sheban,Shay Geula,Nofar Mor,Leehee Weinberger,Segev Naveh Tassa,Vladislav Krupalnik,Bernardo Oldak,Nir Livnat,Shadi Tarazi,Shadi Tawil,Emilie Wildschutz,Shahd Ashouokhi,Lior Lasman,Varda Rotter,Suhair Hanna,Dalit Ben-Yosef,Noa Novershtern,Sergey Viukov,Jacob H Hanna

STAR protocols 1:100183 PubMed33377077

2020

Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Alicia Sanzhez-Mata,Alberto Ferez-Gomez,Elena Gonzalez-Muñoz

Stem cell research 48:101999 PubMed32971459

2020

L1cam curbs the differentiation of adult-born hippocampal neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Grońska-Pęski,Melitta Schachner,Jean M Hébert

Scientific reports 10:7041 PubMed32341445

2020

Non-cell autonomous promotion of astrogenesis at late embryonic stages by constitutive YAP activation.

Applications

Unspecified application

Species

Unspecified reactive species

Dasol Han,Mookwang Kwon,Sun Min Lee,Samuel J Pleasure,Keejung Yoon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IRS1 antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (42)

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