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AB178703

Anti-IRS1 (phospho S1101) + IRS2 (phospho S1149) antibody [EPR2350(2)]

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(2 Publications)

Rabbit Recombinant Monoclonal IRS1 phospho S1101 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Insulin receptor substrate 1, IRS-1, IRS1, Insulin receptor substrate 2, IRS-2, IRS2

1 Images
Western blot - Anti-IRS1 (phospho S1101) + IRS2 (phospho S1149) antibody [EPR2350(2)] (AB178703)
  • WB

Supplier Data

Western blot - Anti-IRS1 (phospho S1101) + IRS2 (phospho S1149) antibody [EPR2350(2)] (AB178703)

All lanes:

Western blot - Anti-IRS1 (phospho S1101) + IRS2 (phospho S1149) antibody [EPR2350(2)] (ab178703) at 1/1000 dilution

Lane 1:

293T cell lysate untreated at 10 µg

Lane 2:

293T cell lysate treated with insulin at 10 µg

Predicted band size: 131 kDa

false

  • Carrier free

    Anti-IRS1 (phospho S1101) + IRS2 (phospho S1149) antibody [EPR2350(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2350(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>ab178703 only detects IRS1 phosphorylated at Serine 1101 and IRS2 phosphorylated at Serine 1149&#46;</p>

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Complementary Products

Assay Kits

AB289646

Human IRS1 ELISA Kit

Recombinant|SimpleStep

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  • 0
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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS2 antibody [EPR904(2)] (AB134101)

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Secondary Antibodies

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Primary Antibodies

AB33780

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Primary Antibodies

AB283689

Anti-Insulin Receptor alpha antibody [EPR23962-157]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (AB283689)

  • 0
  • 0
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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The targets IRS1 and IRS2 also known as Insulin Receptor Substrate 1 and 2 are important adaptor proteins that play an important role in insulin signaling. IRS1 and IRS2 have molecular masses of approximately 180 kDa and 185 kDa respectively. These proteins are expressed in a wide range of tissues but show particularly high expression in insulin-responsive tissues such as muscle liver and adipose tissues. IRS1 and IRS2 function by interacting with activated insulin receptors allowing subsequent recruitment of downstream signaling molecules.
Biological function summary

IRS1 and IRS2 translate insulin receptor activation into metabolic and mitogenic effects. These proteins form signaling complexes that mediate the action of insulin and insulin-like growth factor 1 (IGF-1). IRS1 and IRS2 interact with several signaling molecules including PI3K and GRB2 which are essential for the activation of downstream pathways. Their role is essential in glucose homeostasis cell growth and differentiation. In some contexts IRS1 and IRS2 also participate in signaling pathways influenced by other growth factors.

Pathways

IRS1 and IRS2 have pivotal roles in the insulin signaling and IGF-1 signaling pathways. They facilitate signal transduction from the insulin and IGF-1 receptors impacting both metabolic and growth-related pathways. Within these pathways their interaction with PI3K highlights their importance in glucose uptake and utilization while their connection with SHC and ERK pathways emphasizes their involvement in cell growth and proliferation. The coordinated action of IRS1 and IRS2 helps maintain energy balance and cellular functions.

IRS1 and IRS2 have significant associations with type 2 diabetes and certain cancers. In type 2 diabetes alterations in IRS1 and IRS2 signaling can impair glucose metabolism leading to insulin resistance. Genetic variations or dysregulation in their function often contribute to diabetes susceptibility. Additionally IRS1 and IRS2 are linked to cancer as their pathways overlap with pathways that influence cell growth and survival. Increased signaling can enhance cell proliferation and resistance to apoptosis with relevant proteins like PI3K and AKT further involved in these processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R (PubMed : 7541045, PubMed : 33991522, PubMed : 38625937). Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism (PubMed : 19639489). Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2 (PubMed : 11171109, PubMed : 8265614). Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade (By similarity). Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. Acts a positive regulator of the Wnt/beta-catenin signaling pathway through suppression of DVL2 autophagy-mediated degradation leading to cell proliferation (PubMed : 24616100).
See full target information IRS1 pS1101

Additional targets

Insulin receptor substrate 2 phospho S1149
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Toxics 11: PubMed36976981

2023

Gestational and Lactational Co-Exposure to DEHP and BPA Impairs Hepatic Function via PI3K/AKT/FOXO1 Pathway in Offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Minghan Wang,Yu Wang,Junyuan Han,Zhiwen Duan,Jiye Yin,Rigao Ding,Quanjun Wang

The Journal of cell biology 222: PubMed36946995

2023

The meiotic LINC complex component KASH5 is an activating adaptor for cytoplasmic dynein.

Applications

Unspecified application

Species

Unspecified reactive species

Kirsten E L Garner,Anna Salter,Clinton K Lau,Manickam Gurusaran,Cécile M Villemant,Elizabeth P Granger,Gavin McNee,Philip G Woodman,Owen R Davies,Brian E Burke,Victoria J Allan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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