AB1194

Anti-IRS1 (phospho S307) antibody

Name: Anti-IRS1 (phospho S307) antibody (ab1194) | Abcam
Brand: Abcam Limited
SKU: AB1194
Price: 535 USD
Availability: InStock

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(16 Publications)

Rabbit Polyclonal IRS1 phospho S307 antibody. Suitable for WB and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human Insulin receptor substrate 1 phospho S307 aa 250-350 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Insulin receptor substrate 1, IRS-1, IRS1

1 Images

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Western blot - Anti-IRS1 (phospho S307) antibody (AB1194)

The 180kDa band is believed to correspond to phospho S307 IRS1. Blocking with the immunising peptide removes the 180kDa band (data not shown). A 4-20% Tris-Glycine gel was used, with blocking with 5% goat serum and 0.5% BLOTTO in PBS.

All lanes:

Western blot - Anti-IRS1 (phospho S307) antibody (ab1194) at 1/250 dilution

Lane 1:

293 cell lysate at 25 µg

Lane 2:

293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min. at 25 µg

Secondary

All lanes:

IRDye800 Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

Predicted band size: 131 kDa

Observed band size: 180 kDa,70 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human Insulin receptor substrate 1 phospho S307 aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P35568

Specificity

Recognises a 180kDa band in 293 cells stimulated with anisomysin after serum starvation.

This affinity-purified antibody is directed against the phosphorylated form of human IRS1 protein at the pS307 residue. Reactivity occurs against human IRS1 pS307 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human IRS1 is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with IRS1 from human, mouse, rat, dog and vervet monkey based on 100% homology with the immunizing sequence. Partial reactivity is also expected against IRS1 from pig (94%), bovine (94%) and chicken (88%) sources.Â Reactivity of this antibody with IRS1 from other species is unknown.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This affinity-purified antibody is directed against the phosphorylated form of human IRS1 protein at the pS307 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein IRS1 also known as Insulin Receptor Substrate 1 is an important player in insulin signaling. It is a docking protein that plays a part in the signaling pathway of insulin and insulin-like growth factor (IGF) contributing to glucose homeostasis. The molecular weight of IRS1 is approximately 180 kDa. Expressed in various tissues including muscle liver and adipose tissue IRS1 facilitates the transmission of signals from activated cell surface receptors to intracellular pathways. Notable for its phosphorylation IRS1 undergoes change in state when interacting with receptor kinases impacting its role in cell signaling.

Biological function summary

IRS1 helps mediate the effects of insulin by acting as a downstream effector in the insulin signaling cascade. IRS1 functions in a complex manner binding with PI3K (phosphoinositide 3-kinase) after phosphorylation at specific tyrosine residues. This interaction is important for further signaling events leading to the regulation of glucose uptake lipid synthesis and gene expression. Besides its role in energy balance IRS1 plays a significant part in cell growth and differentiation driven by its ability to relay signals from the insulin and IGF receptors.

Pathways

IRS1 is heavily involved in the insulin signaling pathway and the PI3K-Akt pathway. Upon phosphorylation IRS1 binds to proteins such as the PI3K initiating a cascade that ultimately activates Akt resulting in anabolic processes within cells. The pathway is essential for regulating basic functions like metabolism growth and survival. IRS1's interaction with other related proteins like IR (insulin receptor) and IGFR (insulin-like growth factor receptor) highlights its integrative role in cellular processes related to energy and growth.

IRS1 is implicated in insulin resistance and type 2 diabetes. Changes in IRS1 phosphorylation patterns can disrupt normal insulin signaling leading to impaired glucose uptake and metabolic dysregulation. The protein's dysfunction is linked to obesity forming a connection through its interactions with other proteins like Akt and mTOR. Aberrant IRS1 activity may contribute to altered cell metabolism and growth promoting conditions like diabetes which highlights the importance of IRS1 in maintaining metabolic health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R (PubMed : 7541045, PubMed : 33991522, PubMed : 38625937). Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism (PubMed : 19639489). Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2 (PubMed : 11171109, PubMed : 8265614). Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade (By similarity). Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. Acts a positive regulator of the Wnt/beta-catenin signaling pathway through suppression of DVL2 autophagy-mediated degradation leading to cell proliferation (PubMed : 24616100).

See full target information Insulin receptor substrate 1 phospho S307

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 19:10077-10095 PubMed39371478

2024

Cold Exposure Alleviates T2DM Through Plasma-Derived Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Fu-Xing-Zi Li,Feng Xu,Chang-Chun Li,Li-Min Lei,Su-Kang Shan,Ming-Hui Zheng,Xiao Lin,Bei Guo,Ke-Xin Tang,Jia-Yue Duan,Yun-Yun Wu,Ye-Chi Cao,Jun-Jie Liu,Ling-Qing Yuan

Life science alliance 6: PubMed37059474

2023

Cognitive decline in diabetic mice predisposed to Alzheimer's disease is greater than in wild type.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Carús-Cadavieco,Inés Berenguer López,Alba Montoro Canelo,Miguel A Serrano-Lope,Sandra González-de la Fuente,Begoña Aguado,Alba Fernández-Rodrigo,Takaomi C Saido,Ana Frank García,César Venero,José A Esteban,Francesc Guix,Carlos G Dotti

Molecular nutrition & food research 66:e2200182 PubMed35972028

2022

Calorie Restriction Enhanced Glycogen Metabolism to Compensate for Lipid Insufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Hu,Xinyi Xia,Yue Zong,Yunjie Gu,Li Wei,Jun Yin

Nutrition, metabolism, and cardiovascular diseases : NMCD 32:2655-2668 PubMed36058761

2022

Quercetin ameliorated insulin resistance via regulating METTL3-mediated N6-methyladenosine modification of PRKD2 mRNA in skeletal muscle and C2C12 myocyte cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Jiao,Albert Williams,Ning Wei

BioFactors (Oxford, England) 47:587-599 PubMed33740285

2021

Berberine ameliorates neuronal AD-like change via activating Pi3k/PGCε pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ninghua Wu,Wu Liu,Jiawen Wang,Yanqi Han,Yu Ye,Xiufen Liu,Yuandong Yu,Qingjie Chen,Yongfen Bao,Chao Liu

Chinese medicine 15:125 PubMed33292347

2020

Catechins from oolong tea improve uterine defects by inhibiting STAT3 signaling in polycystic ovary syndrome mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ge Hong,Hao Wu,Shi-Tang Ma,Zhe Su

Molecular medicine reports 21:2603-2614 PubMed32323785

2020

Berberine induces lipolysis in porcine adipocytes by activating the AMP‑activated protein kinase pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqing Yang,Rongsheng Lu,Fangfang Gao,Jie Zhang,Fenglan Liu

Journal of molecular endocrinology 64:181-193 PubMed31990656

2020

PGRN exerts inflammatory effects via SIRT1-NF-κB in adipose insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Huixia Li,Zhuanmin Zhang,Dongxu Feng,Lin Xu,Fang Li,Jiali Liu,Xinxin Jin,Zhuang Qian,Xiaomin Kang,Hongzhi Sun

Plastic and reconstructive surgery. Global open 7:e2339 PubMed31592376

2019

Skin Protein Profile after Major Weight Loss and Its Role in Body Contouring Surgery.

Applications

Unspecified application

Species

Unspecified reactive species

José Renato B Gallo,Larissa B Maschio-Signorini,Celso R B Cabral,Debora A P de Campos Zuccari,Maurício L Nogueira,Antônio R Bozola,Patricia M Cury,Alessandra Vidotto

Molecular therapy. Nucleic acids 18:34-44 PubMed31479923

2019

Exercise Reduces Insulin Resistance in Type 2 Diabetes Mellitus via Mediating the lncRNA MALAT1/MicroRNA-382-3p/Resistin Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Sui-Xin Liu,Fan Zheng,Kang-Ling Xie,Mu-Rong Xie,Ling-Jun Jiang,Ying Cai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-IRS1 (phospho S307) antibody

Western blot - Anti-IRS1 (phospho S307) antibody (AB1194)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

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