Rabbit Polyclonal IRS1 phospho S307 antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human Insulin receptor substrate 1 phospho S307 aa 250-350 conjugated to Keyhole Limpet Haemocyanin.
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- Life science alliance 6:2023Cognitive decline in diabetic mice predisposed to Alzheimer's disease is greater than in wild type.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarta Carús-Cadavieco,Inés Berenguer López,Alba Montoro Canelo,Miguel A Serrano-Lope,Sandra González-de la Fuente,Begoña Aguado,Alba Fernández-Rodrigo,Takaomi C Saido,Ana Frank García,César Venero,José A Esteban,Francesc Guix,Carlos G DottiPubMed 37059474
- Plastic and reconstructive surgery. Global open 7:e23392019Skin Protein Profile after Major Weight Loss and Its Role in Body Contouring Surgery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJosé Renato B Gallo et. al.PubMed 31592376
- Molecular therapy. Nucleic acids 18:34-442019Exercise Reduces Insulin Resistance in Type 2 Diabetes Mellitus via Mediating the lncRNA MALAT1/MicroRNA-382-3p/Resistin Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSui-Xin Liu et. al.PubMed 31479923
- Medical science monitor : international medical jo 25:4655-46642019Effect of Dexmedetomidine-Mediated Insulin-Like Growth Factor 2 (IGF2) Signal Pathway on Immune Function and Invasion and Migration of Cancer Cells in Rats with Ovarian Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHang Tian et. al.PubMed 31230061
- Disease models & mechanisms 11:2018Identification of early indicators of altered metabolism in normal development using a rodent model system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshok Daniel Prabakaran et. al.PubMed 29434026
- Oncology letters 15:447-4522018Oncogenic activity of insulin in the development of non-small cell lung carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Jiang,Hong-Yue Ren,Guo-Jun Geng,Yan-Jun Mi,Yu Liu,Ning Li,Shu-Yu Yang,Dong-Yan ShenPubMed 29387228
- Molecular medicine reports 15:3803-38092017Epigallocatechin-3-gallate ameliorates insulin resistance in hepatocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShan-Bo Ma et. al.PubMed 28393248
- Journal of cell science 130:1147-11572017Endocytic turnover of Rab8 controls cell polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaite Vidal-Quadras et. al.PubMed 28137756
- Physiological reports 2:2014Insulin resistance and muscle insulin receptor substrate-1 serine hyperphosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharles A Stuart et. al.PubMed 25472611
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Insulin receptor substrate 1 phospho S307 aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P35568
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Pig | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig | Dilution info - | Notes - |
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Target data
Function
Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R (PubMed:7541045, PubMed:33991522, PubMed:38625937). Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism (PubMed:19639489). Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2 (PubMed:11171109, PubMed:8265614). Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade (By similarity). Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. Acts a positive regulator of the Wnt/beta-catenin signaling pathway through suppression of DVL2 autophagy-mediated degradation leading to cell proliferation (PubMed:24616100).
Alternative names
Insulin receptor substrate 1, IRS-1, IRS1
Recommended products
Rabbit Polyclonal IRS1 phospho S307 antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human Insulin receptor substrate 1 phospho S307 aa 250-350 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Insulin receptor substrate 1 phospho S307 aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P35568
- Purification technique
- Affinity purification Immunogen
- Specificity
Recognises a 180kDa band in 293 cells stimulated with anisomysin after serum starvation.
This affinity-purified antibody is directed against the phosphorylated form of human IRS1 protein at the pS307 residue. Reactivity occurs against human IRS1 pS307 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human IRS1 is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with IRS1 from human, mouse, rat, dog and vervet monkey based on 100% homology with the immunizing sequence. Partial reactivity is also expected against IRS1 from pig (94%), bovine (94%) and chicken (88%) sources. Reactivity of this antibody with IRS1 from other species is unknown.
- Concentration
- Purification notes
This affinity-purified antibody is directed against the phosphorylated form of human IRS1 protein at the pS307 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein IRS1 also known as Insulin Receptor Substrate 1 is an important player in insulin signaling. It is a docking protein that plays a part in the signaling pathway of insulin and insulin-like growth factor (IGF) contributing to glucose homeostasis. The molecular weight of IRS1 is approximately 180 kDa. Expressed in various tissues including muscle liver and adipose tissue IRS1 facilitates the transmission of signals from activated cell surface receptors to intracellular pathways. Notable for its phosphorylation IRS1 undergoes change in state when interacting with receptor kinases impacting its role in cell signaling.
- Biological function summary
IRS1 helps mediate the effects of insulin by acting as a downstream effector in the insulin signaling cascade. IRS1 functions in a complex manner binding with PI3K (phosphoinositide 3-kinase) after phosphorylation at specific tyrosine residues. This interaction is important for further signaling events leading to the regulation of glucose uptake lipid synthesis and gene expression. Besides its role in energy balance IRS1 plays a significant part in cell growth and differentiation driven by its ability to relay signals from the insulin and IGF receptors.
- Pathways
IRS1 is heavily involved in the insulin signaling pathway and the PI3K-Akt pathway. Upon phosphorylation IRS1 binds to proteins such as the PI3K initiating a cascade that ultimately activates Akt resulting in anabolic processes within cells. The pathway is essential for regulating basic functions like metabolism growth and survival. IRS1's interaction with other related proteins like IR (insulin receptor) and IGFR (insulin-like growth factor receptor) highlights its integrative role in cellular processes related to energy and growth.
- Associated diseases and disorders
IRS1 is implicated in insulin resistance and type 2 diabetes. Changes in IRS1 phosphorylation patterns can disrupt normal insulin signaling leading to impaired glucose uptake and metabolic dysregulation. The protein's dysfunction is linked to obesity forming a connection through its interactions with other proteins like Akt and mTOR. Aberrant IRS1 activity may contribute to altered cell metabolism and growth promoting conditions like diabetes which highlights the importance of IRS1 in maintaining metabolic health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-IRS1 (phospho S307) antibody (ab1194)
The 180kDa band is believed to correspond to phospho S307 IRS1.
Blocking with the immunising peptide removes the 180kDa band (data not shown). A 4-20% Tris-Glycine gel was used, with blocking with 5% goat serum and 0.5% BLOTTO in PBS.All lanes: Western blot - Anti-IRS1 (phospho S307) antibody (ab1194) at 1/250 dilution
Lane 1: 293 cell lysate at 25 µg
Lane 2: 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min. at 25 µg
SecondaryAll lanes: IRDye800 Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 180 kDa, 70 kDa
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