AB5599

Anti-IRS1 (phospho S307) antibody

Name: Anti-IRS1 (phospho S307) antibody (ab5599) | Abcam
Brand: Abcam Limited
SKU: AB5599
Price: 525 USD
Availability: InStock

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(18 Publications)

Rabbit Polyclonal IRS1 phospho S307 antibody. Suitable for ICC, WB and reacts with Human, Mouse samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human Insulin receptor substrate 1 phospho S307 aa 300-350.

View Alternative Names

Insulin receptor substrate 1, IRS-1, IRS1

2 Images

Supplier Data

Immunocytochemistry - Anti-IRS1 (phospho S307) antibody (AB5599)

Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.25% Triton™ X-100 permeabilized T-47D cells (Human ductal breast epithelial tumor cell line) staining IRS1 with ab5599 at 1 μg/ml concentration and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (blue) and F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control uses no primary antibody.

Supplier Data

Western blot - Anti-IRS1 (phospho S307) antibody (AB5599)

Blocking buffer : 5% skimmed milk

All lanes:

Western blot - Anti-IRS1 (phospho S307) antibody (ab5599) at 3 µg/mL

Lane 1:

Jurkat whole cell lysates at 30 µg

Lane 2:

Jurkat treated for 20 minutes with 50 ng/ml of TNF alpha whole cell lysate at 30 µg

Lane 3:

NIH/3T3 whole cell lysates at 30 µg

Lane 4:

NIH/3T3 treated for 20 minutes with 50 ng/ml of TNF alpha whole cell lysates at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) secondary antibody, HRP conjugate at 0.4 µg/mL

Predicted band size: 131 kDa

Observed band size: 131 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Human Insulin receptor substrate 1 phospho S307 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

P35568

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "African green monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein IRS1 also known as Insulin Receptor Substrate 1 is an important player in insulin signaling. It is a docking protein that plays a part in the signaling pathway of insulin and insulin-like growth factor (IGF) contributing to glucose homeostasis. The molecular weight of IRS1 is approximately 180 kDa. Expressed in various tissues including muscle liver and adipose tissue IRS1 facilitates the transmission of signals from activated cell surface receptors to intracellular pathways. Notable for its phosphorylation IRS1 undergoes change in state when interacting with receptor kinases impacting its role in cell signaling.

Biological function summary

IRS1 helps mediate the effects of insulin by acting as a downstream effector in the insulin signaling cascade. IRS1 functions in a complex manner binding with PI3K (phosphoinositide 3-kinase) after phosphorylation at specific tyrosine residues. This interaction is important for further signaling events leading to the regulation of glucose uptake lipid synthesis and gene expression. Besides its role in energy balance IRS1 plays a significant part in cell growth and differentiation driven by its ability to relay signals from the insulin and IGF receptors.

Pathways

IRS1 is heavily involved in the insulin signaling pathway and the PI3K-Akt pathway. Upon phosphorylation IRS1 binds to proteins such as the PI3K initiating a cascade that ultimately activates Akt resulting in anabolic processes within cells. The pathway is essential for regulating basic functions like metabolism growth and survival. IRS1's interaction with other related proteins like IR (insulin receptor) and IGFR (insulin-like growth factor receptor) highlights its integrative role in cellular processes related to energy and growth.

IRS1 is implicated in insulin resistance and type 2 diabetes. Changes in IRS1 phosphorylation patterns can disrupt normal insulin signaling leading to impaired glucose uptake and metabolic dysregulation. The protein's dysfunction is linked to obesity forming a connection through its interactions with other proteins like Akt and mTOR. Aberrant IRS1 activity may contribute to altered cell metabolism and growth promoting conditions like diabetes which highlights the importance of IRS1 in maintaining metabolic health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R (PubMed : 7541045, PubMed : 33991522, PubMed : 38625937). Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism (PubMed : 19639489). Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2 (PubMed : 11171109, PubMed : 8265614). Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade (By similarity). Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. Acts a positive regulator of the Wnt/beta-catenin signaling pathway through suppression of DVL2 autophagy-mediated degradation leading to cell proliferation (PubMed : 24616100).

See full target information Insulin receptor substrate 1 phospho S307

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Biomedical reports 22:99 PubMed40297802

2025

Resveratrol ameliorates high‑fat diet‑induced insulin resistance via the DDIT4/mTOR pathway in skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyan Pan,Gangqiang Xue,Ming Zhao,Ziping Xiang,Dian Liu,Zesen Duan,Chao Wang

iScience 27:111019 PubMed39429784

2024

Behenic acid alleviates inflammation and insulin resistance in gestational diabetes mellitus by regulating TLR4/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kerong Liu,Ying Gu,Xingnan Pan,Sha Chen,Jie Cheng,Le Zhang,Minkai Cao

Frontiers in endocrinology 15:1402905 PubMed39268230

2024

Overfeeding in the early postnatal period aggravates inflammation and hepatic insulin sensitivity in the 5α-dihydrotestosterone-induced animal model of PCOS.

Applications

Unspecified application

Species

Unspecified reactive species

Nataša Veličković,Bojana Mićić,Ana Teofilović,Milena Milovanovic,Mirna Jovanović,Ana Djordjevic,Djuro Macut,Danijela Vojnović Milutinović

Foods (Basel, Switzerland) 13: PubMed38998642

2024

Korean Red Ginseng Improves Oxidative Stress-Induced Hepatic Insulin Resistance via Enhancing Mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Nodir Rustamov,Yuanqiang Ma,Jeong-Su Park,Feng Wang,Hwan Ma,Guoyan Sui,Gahye Moon,Hwan-Soo Yoo,Yoon-Seok Roh

BioFactors (Oxford, England) 50:828-844 PubMed38318672

2024

Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet.

Applications

Unspecified application

Species

Unspecified reactive species

Biljana Bursać,Luisa Bellachioma,Ljupka Gligorovska,Mirna Jovanović,Ana Teofilović,Miloš Vratarić,Danijela Vojnović Milutinović,Alfonso Albacete,Purificación A Martínez-Melgarejo,Camilla Morresi,Elisabetta Damiani,Tiziana Bacchetti,Ana Djordjevic

American journal of physiology. Regulatory, integrative and comparative physiology 325:R523-R533 PubMed37642284

2023

High-fat diet during pregnancy promotes fetal skeletal muscle fatty acid oxidation and insulin resistance in an ovine model.

Applications

Unspecified application

Species

Unspecified reactive species

Asma K Omar,Lance C Li Puma,Luke A Whitcomb,Briana D Risk,Aria C Witt,Jason E Bruemmer,Quinton A Winger,Gerrit J Bouma,Adam J Chicco

Molecular medicine reports 28: PubMed37594055

2023

Resveratrol improves palmitic acid‑induced insulin resistance via the DDIT4/mTOR pathway in C2C12 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyan Pan,Chunqiao Liu,Xing Wang,Ming Zhao,Zhimei Zhang,Xuemei Zhang,Chao Wang,Guangyao Song

International journal of molecular sciences 23: PubMed36012206

2022

AMPK Activation Is Important for the Preservation of Insulin Sensitivity in Visceral, but Not in Subcutaneous Adipose Tissue of Postnatally Overfed Rat Model of Polycystic Ovary Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Bojana Mićić,Ana Teofilović,Ana Djordjevic,Nataša Veličković,Djuro Macut,Danijela Vojnović Milutinović

International journal of molecular sciences 23: PubMed35806430

2022

G6PD Deficiency Is Crucial for Insulin Signaling Activation in Skeletal Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Aiwen Jiang,Hongyun Guo,Xiaoyu Jiang,Jingli Tao,Wangjun Wu,Honglin Liu

Journal of clinical medicine 11: PubMed35329973

2022

Host Metabolic Changes during Mycobacterium Tuberculosis Infection Cause Insulin Resistance in Adult Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Neelam Oswal,Kezia Lizardo,Dhanya Dhanyalayam,Janeesh P Ayyappan,Hariprasad Thangavel,Scott K Heysell,Jyothi F Nagajyothi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-IRS1 (phospho S307) antibody

Immunocytochemistry - Anti-IRS1 (phospho S307) antibody (AB5599)

Western blot - Anti-IRS1 (phospho S307) antibody (AB5599)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

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