AB327340

Anti-IRS1 (phospho S312) antibody [EPR29078-524R]

RabMAb
Recombinant
20ul selling size
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Rabbit Recombinant Monoclonal IRS1 phospho S312 antibody. Suitable for Dot, WB and reacts with Synthetic peptide - Human, Human, Mouse samples.

View Alternative Names

Insulin receptor substrate 1, IRS-1, IRS1

3 Images

Lab

Western blot - Anti-IRS1 (phospho S312) antibody [EPR29078-524R] (AB327340)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody recognizes human IRS1 when phosphorylated at Ser312 (equivalent to Ser307 of mouse IRS1).

All lanes:

Western blot - Anti-IRS1 (phospho S312) antibody [EPR29078-524R] (ab327340) at 1/5000 dilution

Lane 1:

Untreated HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 20 μg (untreated membrane)

Lane 2:

HepG2 treated with 0.5nM insulin for 15 minutes whole cell lysate 20 μg (untreated membrane)

Lane 3:

Untreated HepG2 whole cell lysate 20 μg (alkaline phosphatase treated membrane)

Lane 4:

HepG2 treated with 0.5nM insulin for 15 minutes whole cell lysate 20 μg (alkaline phosphatase treated membrane)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 180 kDa

false

Exposure time: 180s

Lab

Dot Blot - Anti-IRS1 (phospho S312) antibody [EPR29078-524R] (AB327340)

Dot blot analysis of IRS1 using ab327340 at 1 : 1000 (0.509 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : IRS1 (phospho S312) peptide

Lane 2 : IRS1 non-phospho peptide

Lane 3 : IRS1 (phospho T311) peptide

Lane 4 : IRS1 (phospho S307 + T309 + T311 + S315) peptide

Lane 5 : IRS1 (phospho S307) peptide

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-IRS1 (phospho S312) antibody [EPR29078-524R] (ab327340) at 1/1000 dilution

Lane 1:

IRS1 (phospho S312) peptide

Lane 2:

IRS1 non-phospho peptide

Lane 3:

IRS1 (phospho T311) peptide

Lane 4:

IRS1 (phospho S307 + T309 + T311 + S315) peptide

Lane 5:

IRS1 (phospho S307) peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Lab

Western blot - Anti-IRS1 (phospho S312) antibody [EPR29078-524R] (AB327340)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody recognizes mouse IRS1 when phosphorylated at Ser307 (equivalent to Ser312 of human IRS1).

All lanes:

Western blot - Anti-IRS1 (phospho S312) antibody [EPR29078-524R] (ab327340) at 1/1000 dilution

Lane 1:

C2C12 (mouse myoblast) was starved for 2 hours, whole cell lysate 20 μg (untreated membrane)

Lane 2:

C2C12 was starved for 2 hours, and then treated with 50nM insulin for 15 minutes whole cell lysate 20 μg (untreated membrane)

Lane 3:

C2C12 was starved for 2 hours, whole cell lysate 20 μg (alkaline phosphatase treated membrane)

Lane 4:

C2C12 was starved for 2 hours, and then treated with 50nM insulin for 15 minutes whole cell lysate 20 μg (alkaline phosphatase treated membrane)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 180 kDa

false

Exposure time: 103s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29078-524R

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects human IRS1 phosphorylated at Ser312 (corresponding to Ser307 in mouse IRS1). It does not cross-react with unmodified human IRS1 or with IRS1 phosphorylated at Ser307, Thr309, Thr311, or Ser315, as demonstrated by dot blot.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R (PubMed : 7541045, PubMed : 33991522, PubMed : 38625937). Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism (PubMed : 19639489). Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2 (PubMed : 11171109, PubMed : 8265614). Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade (By similarity). Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. Acts a positive regulator of the Wnt/beta-catenin signaling pathway through suppression of DVL2 autophagy-mediated degradation leading to cell proliferation (PubMed : 24616100).

See full target information IRS1 pS312

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com