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Rabbit Recombinant Multiclonal IRS1 phospho S312 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human IRS1 phospho S312.

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Images

Western blot - Anti-IRS1 (phospho S312) antibody [RP23040108] (AB313437), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IRS1 (phospho S312) antibody [RP23040108] (AB313437), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • Synthetic Peptide within Human IRS1 phospho S312. Database link P35568

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1-3 µg/mL
Notes

-

Target data

Function

Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R (PubMed:7541045, PubMed:33991522, PubMed:38625937). Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism (PubMed:19639489). Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2 (PubMed:11171109, PubMed:8265614). Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade (By similarity). Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. Acts a positive regulator of the Wnt/beta-catenin signaling pathway through suppression of DVL2 autophagy-mediated degradation leading to cell proliferation (PubMed:24616100).

Alternative names

Insulin receptor substrate 1, IRS-1, IRS1

Recommended products

Rabbit Recombinant Multiclonal IRS1 phospho S312 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human IRS1 phospho S312.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • Synthetic Peptide within Human IRS1 phospho S312. Database link P35568
Clone number
RP23040108
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-IRS1 (phospho S312) antibody [RP23040108] (ab313437), expandable thumbnail

    Western blot - Anti-IRS1 (phospho S312) antibody [RP23040108] (ab313437)

    All lanes: Western blot - Anti-IRS1 (phospho S312) antibody [RP23040108] (ab313437) at 1 µg/mL

    Lane 1: HepG2 cell lysate at 30 µg

    Lane 2: HepG2 treated with IGF1 (100 ng/mL/20 min) cell lysate at 30 µg

    Lane 3: MCF7 cell lysate at 30 µg

    Lane 4: MCF7 treated with Insulin (100 nM/10 min) cell lysate at 30 µg

    Lane 5: C2C12 cell lysate at 30 µg

    Lane 6: C2C12 treated with Insulin (100 nM/ 10 minutes) cell lysate at 30 µg

    Lane 7: phosphopeptide incubated (10 µg/ml) HepG2 cell lysate at 30 µg

    Lane 8: phosphopeptide incubated (10 µg/ml) HepG2 treated with IGF1 (100 ng/mL/20 min) cell lysate at 30 µg

    Lane 9: phosphopeptide incubated (10 µg/ml) MCF7 cell lysate at 30 µg

    Lane 10: phosphopeptide incubated (10 µg/ml) MCF7 treated with Insulin (100 nM/10 min) cell lysate at 30 µg

    Lane 11: phosphopeptide incubated (10 µg/ml) C2C12 cell lysate at 30 µg

    Lane 12: phosphopeptide incubated (10 µg/ml) C2C12 treated with Insulin (100 nM/10 min) cell lysate at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 131.591 kDa

    Observed band size: 180 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IRS1 (phospho S312) antibody [RP23040108] (ab313437), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IRS1 (phospho S312) antibody [RP23040108] (ab313437)

    Immunofluorescent analysis of MCF7 cells treated with IGF (100 ng/mL/20 min) fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling IRS with ab313437 at 1 ?g/mL followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2500 dilution. Panel a) shows representative cells that were stained for detection and localization of IRS (pS312) protein (green), Panel b) is stained for Nuclei (blue) using DAPI at 1/50 dilution. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor 594 Phalloidin at 1/200 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of IRS (pS312). Panel e) represents cells without treatment and panel f) represents control cells with no primary antibody to assess background.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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