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Rabbit Recombinant Monoclonal IRS1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment - Human samples. Cited in 22 publications.

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Images

Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777), expandable thumbnail
  • Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Tested
Recombinant fragment - Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Recombinant fragment - Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R (PubMed:7541045, PubMed:33991522, PubMed:38625937). Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism (PubMed:19639489). Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2 (PubMed:11171109, PubMed:8265614). Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade (By similarity). Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. Acts a positive regulator of the Wnt/beta-catenin signaling pathway through suppression of DVL2 autophagy-mediated degradation leading to cell proliferation (PubMed:24616100).

Additional Targets

Insulin receptor substrate 2

Alternative names

Insulin receptor substrate 1, IRS-1, IRS1

Recommended products

Rabbit Recombinant Monoclonal IRS1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment - Human samples. Cited in 22 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP263Y
Purification technique
Affinity purification Protein A
Specificity

The immunogen used for this product shares 78% homology with IRS2. Cross-reactivity with this protein has not been confirmed experimentally.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The targets IRS1 and IRS2 also known as Insulin Receptor Substrate 1 and 2 are important adaptor proteins that play an important role in insulin signaling. IRS1 and IRS2 have molecular masses of approximately 180 kDa and 185 kDa respectively. These proteins are expressed in a wide range of tissues but show particularly high expression in insulin-responsive tissues such as muscle liver and adipose tissues. IRS1 and IRS2 function by interacting with activated insulin receptors allowing subsequent recruitment of downstream signaling molecules.

Biological function summary

IRS1 and IRS2 translate insulin receptor activation into metabolic and mitogenic effects. These proteins form signaling complexes that mediate the action of insulin and insulin-like growth factor 1 (IGF-1). IRS1 and IRS2 interact with several signaling molecules including PI3K and GRB2 which are essential for the activation of downstream pathways. Their role is essential in glucose homeostasis cell growth and differentiation. In some contexts IRS1 and IRS2 also participate in signaling pathways influenced by other growth factors.

Pathways

IRS1 and IRS2 have pivotal roles in the insulin signaling and IGF-1 signaling pathways. They facilitate signal transduction from the insulin and IGF-1 receptors impacting both metabolic and growth-related pathways. Within these pathways their interaction with PI3K highlights their importance in glucose uptake and utilization while their connection with SHC and ERK pathways emphasizes their involvement in cell growth and proliferation. The coordinated action of IRS1 and IRS2 helps maintain energy balance and cellular functions.

Associated diseases and disorders

IRS1 and IRS2 have significant associations with type 2 diabetes and certain cancers. In type 2 diabetes alterations in IRS1 and IRS2 signaling can impair glucose metabolism leading to insulin resistance. Genetic variations or dysregulation in their function often contribute to diabetes susceptibility. Additionally IRS1 and IRS2 are linked to cancer as their pathways overlap with pathways that influence cell growth and survival. Increased signaling can enhance cell proliferation and resistance to apoptosis with relevant proteins like PI3K and AKT further involved in these processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777), expandable thumbnail

    Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777)

    Blocking and dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777) at 1/10000 dilution

    All lanes: HEK293 whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 170 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling IRS1 with purified ab40777 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777)

    Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling IRS1 with purified ab40777 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/250) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777), expandable thumbnail

    Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777)

    Blocking and dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777)

    All lanes: HeLa whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 52 kDa

    Observed band size: 170 kDa

  • Flow Cytometry (Intracellular) - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777)

    Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling IRS1 with unpurified ab40777 at 1/90 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777), expandable thumbnail

    Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777)

    All lanes: Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777) at 1/1000 dilution

    All lanes: HeLa cell lysate at 10 µg

    Observed band size: 170 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma labelling IRS1 with unpurified ab40777.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777), expandable thumbnail

    Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777)

    Blocking and diluting buffer and concentration: 5% NFDM /TBST.

    Exposure time: Lane 1, 2, 4, 5 , 6: 1 second. Lane 3: 80 seconds.

    Lanes 1 - 3: Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777) at 1/1000 dilution

    Lanes 4 - 6: Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606) at 1/1000 dilution

    Lanes 1 and 4: HEK-293T transfected with an empty vector whole cell lysate at 20 µg

    Lanes 2 and 5: HEK-293T transfected with a human IRS1 expression vector containing a Myc/DDK tag whole cell lysate at 20 µg

    Lanes 3 and 6: HEK-293T transfected with a human IRS2 expression vector containing a Myc/DDK tag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 170 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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