AB52622

Anti-IRS4 antibody [EP907Y]

RabMAb
Recombinant
What is this?

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(9 Publications)

Rabbit Recombinant Monoclonal IRS4 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 9 publications.

View Alternative Names

Insulin receptor substrate 4, IRS-4, 160 kDa phosphotyrosine protein, Phosphoprotein of 160 kDa, py160, pp160, IRS4

2 Images

Lab

Immunoprecipitation - Anti-IRS4 antibody [EP907Y] (AB52622)

Purified ab52622 at 1/20 dilution (0.5μg) immunoprecipitating IRS4 in HEK-293 whole cell lysate.
Lane 1 (input) : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab52622 + HEK-293 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab52622 in HEK-293 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 133 kDa

All lanes:

Immunoprecipitation - Anti-IRS4 antibody [EP907Y] (ab52622)

Predicted band size: 134 kDa

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Unknown

Western blot - Anti-IRS4 antibody [EP907Y] (AB52622)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-IRS4 antibody [EP907Y] (ab52622) at 1/10000 dilution

All lanes:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 134 kDa

Observed band size: 133 kDa

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Carrier free

Anti-IRS4 antibody [EP907Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP907Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/20 - 1/100", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "For unpurified use at 1/5000 dilution", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The insulin receptor substrate 4 (IRS4) is a protein that plays a significant role in transmitting signals from insulin and insulin-like growth factor receptors to downstream pathways. It has a molecular mass of approximately 130 kDa. IRS4 is mainly expressed in the hypothalamus pituitary gland and several other organs such as liver and adipose tissues. IRS4 interacts with various signaling intermediates through its phosphotyrosine-binding domains therefore acting as a docking protein that facilitates signaling cascades.

Biological function summary

The role of IRS4 extends beyond signal transduction. It participates in the regulation of growth survival and metabolism. Although not part of a permanent protein complex IRS4 temporarily binds to receptors and adaptor proteins during signal transduction processes. The interactions allow IRS4 to integrate signals from different receptors to produce coordinated cellular responses particularly in energy homeostasis and glucose metabolism.

Pathways

IRS4 serves important functions in the insulin signaling and growth hormone signaling pathways. Within the insulin signaling pathway IRS4 acts as a central node linking insulin receptors to the activation of phosphoinositide 3-kinase (PI3K) which subsequently leads to activation of protein kinase B (AKT) and mitogen-activated protein kinase (MAPK) pathways. Related proteins in these cascades include other insulin receptor substrates such as IRS1 and IRS2 which share overlapping roles but may exhibit tissue-specific functions.

IRS4 exhibits associations with metabolic conditions such as diabetes and obesity. Alterations in IRS4 expression or function can disrupt normal insulin signaling contributing to insulin resistance and metabolic dysregulation. IRS4 also shows connectiveness with proteins like PI3K and AKT which are frequently implicated in these metabolic disorders. Dissection of IRS4's role could potentially offer insights for therapeutic strategies targeting these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGF1 signaling pathway by suppressing the function of IRS1 and IRS2.

See full target information Insulin receptor substrate 4

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

npj metabolic health and disease 3:8 PubMed40052110

2025

AMPK phosphosite profiling by label-free mass spectrometry reveals a multitude of mTORC1-regulated substrates.

Applications

Unspecified application

Species

Unspecified reactive species

William J Smiles,Ashley J Ovens,Dingyi Yu,Naomi X Y Ling,Andrea C Poblete Goycoolea,Kaitlin R Morrison,Emmanuel O Murphy,Astrid Glaser,Sophie F Monks O'Byrne,Scott Taylor,Alistair M Chalk,Carl R Walkley,Luke M McAloon,John W Scott,Bruce E Kemp,Ashfaqul Hoque,Christopher G Langendorf,Janni Petersen,Sandra Galic,Jonathan S Oakhill

Cancer management and research 12:3131-3144 PubMed32440211

2020

Long Noncoding RNA Promotes the Malignancy of Melanoma by Promoting the Expression of IRS4 Through Competitive Binding to microRNA-493.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Pengzhen Lei,Weihui Zeng,Jianwu Gao,Na Wu

Theranostics 8:3643-3653 PubMed30026872

2018

Phosphorylation of IRS4 by CK1γ2 promotes its degradation by CHIP through the ubiquitin/lysosome pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinchun Li,Li Zhong,Zhuo Wang,Huiming Chen,Dan Liao,Ruhua Zhang,Hongyu Zhang,Tiebang Kang

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 39:1010428317701640 PubMed28475006

2017

Downregulation of miR-493 promoted melanoma proliferation by suppressing IRS4 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Aili Cui,Zhehu Jin,Zhonggao Gao,Mingji Jin,Lianhua Zhu,Lianhua Li,Chenglong Jin,Yinghua An

Nature genetics 49:65-74 PubMed27869826

2016

Pan-cancer analysis of somatic copy-number alterations implicates IRS4 and IGF2 in enhancer hijacking.

Applications

Unspecified application

Species

Unspecified reactive species

Joachim Weischenfeldt,Taronish Dubash,Alexandros P Drainas,Balca R Mardin,Yuanyuan Chen,Adrian M Stütz,Sebastian M Waszak,Graziella Bosco,Ann Rita Halvorsen,Benjamin Raeder,Theocharis Efthymiopoulos,Serap Erkek,Christine Siegl,Hermann Brenner,Odd Terje Brustugun,Sebastian M Dieter,Paul A Northcott,Iver Petersen,Stefan M Pfister,Martin Schneider,Steinar K Solberg,Erik Thunissen,Wilko Weichert,Thomas Zichner,Roman Thomas,Martin Peifer,Aslaug Helland,Claudia R Ball,Martin Jechlinger,Rocio Sotillo,Hanno Glimm,Jan O Korbel

The Journal of biological chemistry 289:26302-13 PubMed25100728

2014

Insulin receptor substrate-4 binds to Slingshot-1 phosphatase and promotes cofilin dephosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuta Homma,Shin-ichiro Kanno,Kazutaka Sasaki,Michiru Nishita,Akira Yasui,Tomoichiro Asano,Kazumasa Ohashi,Kensaku Mizuno

Journal of neuroendocrinology 25:292-301 PubMed22935015

2012

A novel transcript is up-regulated by fasting in the hypothalamus and enhances insulin signalling.

Applications

Unspecified application

Species

Unspecified reactive species

B Chai,J-Y Li,D Fritze,W Zhang,Z Xia,M W Mulholland

International journal of cancer 128:487-91 PubMed20340132

2010

The t(X;6) in subungual exostosis results in transcriptional deregulation of the gene for insulin receptor substrate 4.

Applications

Unspecified application

Species

Unspecified reactive species

Fredrik Mertens,Emely Möller,Nils Mandahl,Piero Picci,Antonio R Perez-Atayde,Ignace Samson,Raf Sciot,Maria Debiec-Rychter

British journal of haematology 144:546-51 PubMed19055661

2008

The t(X;7)(q22;q34) in paediatric T-cell acute lymphoblastic leukaemia results in overexpression of the insulin receptor substrate 4 gene through illegitimate recombination with the T-cell receptor beta locus.

Applications

Species

Human

Kristina Karrman,Eigil Kjeldsen,Carin Lassen,Margareth Isaksson,Josef Davidsson,Anna Andersson,Henrik Hasle,Thoas Fioretos,Bertil Johansson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IRS4 antibody [EP907Y]

Immunoprecipitation - Anti-IRS4 antibody [EP907Y] (AB52622)

Western blot - Anti-IRS4 antibody [EP907Y] (AB52622)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise