AB284692

Anti-IRS4 antibody [EP907Y] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal IRS4 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

Insulin receptor substrate 4, IRS-4, 160 kDa phosphotyrosine protein, Phosphoprotein of 160 kDa, py160, pp160, IRS4

2 Images

Lab

Immunoprecipitation - Anti-IRS4 antibody [EP907Y] - BSA and Azide free (AB284692)

This data was developed using ab52622, the same antibody clone in a different buffer formulation.Purified ab52622 at 1/20 dilution (0.5µg) immunoprecipitating IRS4 in HEK-293 whole cell lysate.
Lane 1 (input) : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10µg
Lane 2 (+) : ab52622 + HEK-293 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab52622 in HEK-293 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 133 kDa

All lanes:

Immunoprecipitation - Anti-IRS4 antibody [EP907Y] (<a href='/en-us/products/primary-antibodies/irs4-antibody-ep907y-ab52622'>ab52622</a>)

Predicted band size: 134 kDa

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Western blot - Anti-IRS4 antibody [EP907Y] - BSA and Azide free (AB284692)

This data was developed using ab52622, the same antibody clone in a different buffer formulation.

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-IRS4 antibody [EP907Y] (<a href='/en-us/products/primary-antibodies/irs4-antibody-ep907y-ab52622'>ab52622</a>) at 1/10000 dilution

All lanes:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 134 kDa

Observed band size: 133 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP907Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab284692 is the carrier-free version of ab52622

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The insulin receptor substrate 4 (IRS4) is a protein that plays a significant role in transmitting signals from insulin and insulin-like growth factor receptors to downstream pathways. It has a molecular mass of approximately 130 kDa. IRS4 is mainly expressed in the hypothalamus pituitary gland and several other organs such as liver and adipose tissues. IRS4 interacts with various signaling intermediates through its phosphotyrosine-binding domains therefore acting as a docking protein that facilitates signaling cascades.

Biological function summary

The role of IRS4 extends beyond signal transduction. It participates in the regulation of growth survival and metabolism. Although not part of a permanent protein complex IRS4 temporarily binds to receptors and adaptor proteins during signal transduction processes. The interactions allow IRS4 to integrate signals from different receptors to produce coordinated cellular responses particularly in energy homeostasis and glucose metabolism.

Pathways

IRS4 serves important functions in the insulin signaling and growth hormone signaling pathways. Within the insulin signaling pathway IRS4 acts as a central node linking insulin receptors to the activation of phosphoinositide 3-kinase (PI3K) which subsequently leads to activation of protein kinase B (AKT) and mitogen-activated protein kinase (MAPK) pathways. Related proteins in these cascades include other insulin receptor substrates such as IRS1 and IRS2 which share overlapping roles but may exhibit tissue-specific functions.

IRS4 exhibits associations with metabolic conditions such as diabetes and obesity. Alterations in IRS4 expression or function can disrupt normal insulin signaling contributing to insulin resistance and metabolic dysregulation. IRS4 also shows connectiveness with proteins like PI3K and AKT which are frequently implicated in these metabolic disorders. Dissection of IRS4's role could potentially offer insights for therapeutic strategies targeting these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGF1 signaling pathway by suppressing the function of IRS1 and IRS2.

See full target information Insulin receptor substrate 4

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IRS4 antibody [EP907Y] - BSA and Azide free

Immunoprecipitation - Anti-IRS4 antibody [EP907Y] - BSA and Azide free (AB284692)

Western blot - Anti-IRS4 antibody [EP907Y] - BSA and Azide free (AB284692)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise