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AB126057

Anti-IRSp53 antibody

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(3 Publications)

Rabbit Polyclonal IRSp53 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Brain-specific angiogenesis inhibitor 1-associated protein 2 aa 1-200.

View Alternative Names

BAR/IMD domain-containing adapter protein 2, Brain-specific angiogenesis inhibitor 1-associated protein 2, Fas ligand-associated factor 3, Insulin receptor substrate p53/p58, Insulin receptor substrate protein of 53 kDa, BAI-associated protein 2, BAI1-associated protein 2, Protein BAP2, FLAF3, IRS-58, IRSp53/58, IRSp53, Insulin receptor substrate p53, BAIAP2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-IRSp53 antibody (AB126057)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IRSp53 antibody (AB126057)

ab126057, at 1/500 dilution, staining IRSp53 in methanol-fixed A431 cells by Immunofluorescence (green).
Alpha-tubulin filaments are stained in red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRSp53 antibody (AB126057)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRSp53 antibody (AB126057)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for BAIAP2 protein at cytoplasm with ab126057 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-IRSp53 antibody (AB126057)
  • WB

Unknown

Western blot - Anti-IRSp53 antibody (AB126057)

7.5% SDS PAGE

All lanes:

Western blot - Anti-IRSp53 antibody (ab126057) at 1/2000 dilution

All lanes:

SK N SH whole cell lysate at 30 µg

Predicted band size: 57 kDa,61 kDa

false

Western blot - Anti-IRSp53 antibody (AB126057)
  • WB

Supplier Data

Western blot - Anti-IRSp53 antibody (AB126057)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-IRSp53 antibody (ab126057) at 1/10000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Brain-specific angiogenesis inhibitor 1-associated protein 2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9UQB8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRSp53 also known as BAIAP2 (Brain-Specific Angiogenesis Inhibitor 1-Associated Protein 2) is a protein with a molecular mass of approximately 58 kDa. It acts as an important regulator of actin cytoskeleton dynamics facilitating the formation of membrane protrusions like filopodia and lamellipodia. IRSp53 is expressed ubiquitously in various tissues with higher levels observed in the brain. It contains several domains including the IMD (I-BAR) domain which is involved in membrane curvature and actin binding.
Biological function summary

IRSp53 participates in the modulation of cell shape and motility. It is a component of the protein complexes that couple membrane dynamics to cytoskeleton remodeling. Through its interaction with proteins like CDC42 and Rac1 IRSp53 guides the rearrangement of actin filaments affecting cell movement and shape. These abilities make it significant for cellular processes requiring changes in cell structure such as neurodevelopment and wound healing.

Pathways

IRSp53 is central to the regulation of cell motility pathways and signaling processes. It plays an important role in the WAVE (WASP family verprolin-homologous protein) regulatory complex which influences actin polymerization. The protein interacts with Rac1 and N-WASP to mediate pathways leading to the formation of actin-rich structures. This modulation of actin dynamics links IRSp53 to the broader MAPK/ERK signaling pathway impacting cellular proliferation and differentiation.

IRSp53 has been linked to neurological and oncological conditions. Alterations in its expression or function have connections with autism spectrum disorders and glioblastoma. In these disorders IRSp53's interaction with proteins like BAIAP2L1 can influence signal transduction and cytoskeletal organization which may contribute to disease pathology. Understanding IRSp53's roles could offer insights into its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection. Participates in actin bundling when associated with EPS8, promoting filopodial protrusions.
See full target information Brain-specific angiogenesis inhibitor 1-associated protein 2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1012564 PubMed40067817

2025

The CLIC/GEEC pathway regulates particle uptake and formation of the virus-containing compartment (VCC) in HIV-1-infected macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Kathleen Candor,Lingmei Ding,Sai Balchand,Jason E Hammonds,Paul Spearman

Experimental and therapeutic medicine 22:1163 PubMed34504608

2021

miR-184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1.

Applications

Unspecified application

Species

Unspecified reactive species

Gui-Geng Tan,Chang Xu,Wei-Kang Zhong,Chuan-Yun Wang

BioMed research international 2021:3210586 PubMed33506012

2021

Insulin Receptor Substrate p53 Ameliorates High-Glucose-Induced Activation of NF-B and Impaired Mobility of HUVECs.

Applications

Unspecified application

Species

Unspecified reactive species

Fen Liu,Yubin Chen,Shi Zhao,Mei Li,Fanyan Luo,Can-E Tang
View all publications
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Product promise

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