AB15697

Anti-IRSp53 antibody - C-terminal

Name: Anti-IRSp53 antibody - C-terminal (ab15697) | Abcam
Brand: Abcam Limited
SKU: AB15697
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Goat Polyclonal IRSp53 antibody. C-terminal. Suitable for WB and reacts with Human, Mouse, Dog samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human Brain-specific angiogenesis inhibitor 1-associated protein 2 aa 500-550.

View Alternative Names

BAR/IMD domain-containing adapter protein 2, Brain-specific angiogenesis inhibitor 1-associated protein 2, Fas ligand-associated factor 3, Insulin receptor substrate p53/p58, Insulin receptor substrate protein of 53 kDa, BAI-associated protein 2, BAI1-associated protein 2, Protein BAP2, FLAF3, IRS-58, IRSp53/58, IRSp53, Insulin receptor substrate p53, BAIAP2

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Western blot - Anti-IRSp53 antibody - C-terminal (AB15697)

ab15697 staining (1μg/ml) of human brain lysate (RIPA buffer, 35μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

All lanes:

Western blot - Anti-IRSp53 antibody - C-terminal (ab15697) at 1 µg/mL

All lanes:

Lysates prepared from human brain at 35 µg

Predicted band size: 61 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Dog

Applications

applications

Immunogen

Synthetic Peptide within Human Brain-specific angiogenesis inhibitor 1-associated protein 2 aa 500-550. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." } } }

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Product details

This antibody is expected to recognise the human isoform 1 only of this protein.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.05% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRSp53 also known as BAIAP2 (Brain-Specific Angiogenesis Inhibitor 1-Associated Protein 2) is a protein with a molecular mass of approximately 58 kDa. It acts as an important regulator of actin cytoskeleton dynamics facilitating the formation of membrane protrusions like filopodia and lamellipodia. IRSp53 is expressed ubiquitously in various tissues with higher levels observed in the brain. It contains several domains including the IMD (I-BAR) domain which is involved in membrane curvature and actin binding.

Biological function summary

IRSp53 participates in the modulation of cell shape and motility. It is a component of the protein complexes that couple membrane dynamics to cytoskeleton remodeling. Through its interaction with proteins like CDC42 and Rac1 IRSp53 guides the rearrangement of actin filaments affecting cell movement and shape. These abilities make it significant for cellular processes requiring changes in cell structure such as neurodevelopment and wound healing.

Pathways

IRSp53 is central to the regulation of cell motility pathways and signaling processes. It plays an important role in the WAVE (WASP family verprolin-homologous protein) regulatory complex which influences actin polymerization. The protein interacts with Rac1 and N-WASP to mediate pathways leading to the formation of actin-rich structures. This modulation of actin dynamics links IRSp53 to the broader MAPK/ERK signaling pathway impacting cellular proliferation and differentiation.

IRSp53 has been linked to neurological and oncological conditions. Alterations in its expression or function have connections with autism spectrum disorders and glioblastoma. In these disorders IRSp53's interaction with proteins like BAIAP2L1 can influence signal transduction and cytoskeletal organization which may contribute to disease pathology. Understanding IRSp53's roles could offer insights into its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection. Participates in actin bundling when associated with EPS8, promoting filopodial protrusions.

See full target information Brain-specific angiogenesis inhibitor 1-associated protein 2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40548551

2025

PDZ-directed substrate recruitment is the primary determinant of specific 4E-BP1 dephosphorylation by PP1-Neurabin.

Applications

Unspecified application

Species

Unspecified reactive species

Roman O Fedoryshchak,Karim El-Bouri,Dhira Joshi,Stephane Mouilleron,Richard Treisman

eLife 9: PubMed32975518

2020

Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme.

Applications

Unspecified application

Species

Unspecified reactive species

Roman O Fedoryshchak,Magdalena Přechová,Abbey M Butler,Rebecca Lee,Nicola O'Reilly,Helen R Flynn,Ambrosius P Snijders,Noreen Eder,Sila Ultanir,Stephane Mouilleron,Richard Treisman

Molecular cell 55:436-50 PubMed25042806

2014

An AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan M Goodwin,Robert U Svensson,Hua Jane Lou,Monte M Winslow,Benjamin E Turk,Reuben J Shaw

The Journal of cell biology 192:525-40 PubMed21282462

2011

The serine/threonine kinase Par1b regulates epithelial lumen polarity via IRSp53-mediated cell-ECM signaling.

Applications

Species

Dog

David Cohen,Dawn Fernandez,Francisco Lázaro-Diéguez,Anne Müsch

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IRSp53 antibody - C-terminal

Western blot - Anti-IRSp53 antibody - C-terminal (AB15697)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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