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AB250260

Anti-IRX1 antibody [EPR13139] - BSA and Azide free

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Rabbit Recombinant Monoclonal IRX1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

IRXA1, IRX1, Iroquois-class homeodomain protein IRX-1, Homeodomain protein IRXA1, Iroquois homeobox protein 1

2 Images
Western blot - Anti-IRX1 antibody [EPR13139] - BSA and Azide free (AB250260)
  • WB

Supplier Data

Western blot - Anti-IRX1 antibody [EPR13139] - BSA and Azide free (AB250260)

This data was developed using ab180860, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-IRX1 antibody [EPR13139] (<a href='/en-us/products/primary-antibodies/irx1-antibody-epr13139-ab180860'>ab180860</a>) at 1/1000 dilution

Lane 1:

Human stomach lysate at 10 µg

Lane 2:

Human small intestine lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Predicted band size: 50 kDa

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Immunoprecipitation - Anti-IRX1 antibody [EPR13139] - BSA and Azide free (AB250260)
  • IP

Supplier Data

Immunoprecipitation - Anti-IRX1 antibody [EPR13139] - BSA and Azide free (AB250260)

This data was developed using ab180860, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from (1) Human stomach lysate or (2) 1X PBS (negative control) immunoprecipitated using ab180860 at 1/10 dilution; HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

All lanes:

Immunoprecipitation - Anti-IRX1 antibody [EPR13139] (<a href='/en-us/products/primary-antibodies/irx1-antibody-epr13139-ab180860'>ab180860</a>)

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13139

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250260 is the carrier-free version of ab180860.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRX1 also known as Iroquois homeobox 1 functions mechanically as a transcription factor. With a mass of approximately 51 kDa this protein plays an essential role in regulating gene expression during embryonic development. IRX1 expresses mainly in the heart central nervous system and various tissues involved in early development. The regulation and expression patterns make it an interesting target for developmental biology studies.
Biological function summary

IRX1 regulates tissue-specific gene expression and contributes to the establishment of body plans. As part of the Iroquois homeobox gene family it often functions in concert with other members to form a transcriptional complex. This complex is important for the proper development of several organs and systems including heart and brain structures. Its role in the morphogenesis of these tissues highlights its significance in developmental processes.

Pathways

IRX1 integrates into key developmental signaling pathways such as the Wnt signaling and Hedgehog pathways. These pathways are essential for cellular proliferation differentiation and patterning. IRX1 interacts with proteins like GLI1 and β-catenin which are pivotal within these pathways to modulate transcription of target genes. Its involvement supports neurodevelopmental and cardiac developmental processes.

IRX1 displays associations with certain congenital heart defects and oncological conditions including gastric cancer. Studies show that IRX1 dysregulation may lead to malformations in cardiac development linking it to heart defects. Additionally IRX1 interacts with factors like E2F1 in cancer pathways to influence tumor progression suggesting a potential role in tumorigenesis. Understanding these connections could facilitate the development of therapeutic interventions for conditions involving IRX1.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information IRX1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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