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Rabbit Recombinant Monoclonal IRX5 antibody. Suitable for IP, WB and reacts with Transfected cell lysate - Mouse, Mouse samples.

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Images

Immunoprecipitation - Anti-IRX5 antibody [EPR22724-43] (AB246494), expandable thumbnail
  • Western blot - Anti-IRX5 antibody [EPR22724-43] (AB246494), expandable thumbnail
  • Western blot - Anti-IRX5 antibody [EPR22724-43] (AB246494), expandable thumbnail
  • Western blot - Anti-IRX5 antibody [EPR22724-43] (AB246494), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBFlow Cyt (Intra)ICC/IFIHC-P
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Expected
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Transfected cell lysate - Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell lysate - Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell lysate - Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Target data

Function

Establishes the cardiac repolarization gradient by its repressive actions on the KCND2 potassium-channel gene. Required for retinal cone bipolar cell differentiation. May regulate contrast adaptation in the retina and control specific aspects of visual function in circuits of the mammalian retina. Involved in craniofacial and gonadal development (By similarity). Modulates the migration of progenitor cell populations in branchial arches and gonads by repressing CXCL12.

Alternative names

Irxb2, Irx5, Iroquois-class homeodomain protein IRX-5, Homeodomain protein IRXB2, Iroquois homeobox protein 5

Recommended products

Rabbit Recombinant Monoclonal IRX5 antibody. Suitable for IP, WB and reacts with Transfected cell lysate - Mouse, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22724-43
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

IRX5 also known as Iroquois Homeobox 5 is a transcription factor with a molecular mass of approximately 50 kDa. It is part of the Iroquois (Irx) homeobox gene family which plays important roles in embryonic development. IRX5’s expression is mainly found in the heart brain and retina tissues. As a transcription factor it binds to specific DNA sequences regulating the transcription of various genes and influencing cell differentiation and tissue development.

Biological function summary

The function of IRX5 impacts cellular growth and patterning within tissues. It plays a significant role in cardiogenesis retinal differentiation and neuronal development. IRX5 does not operate alone; it forms part of the complex regulatory networks that guide development. During heart development IRX5’s expression patterns guide the proper formation of cardiac structures while in the retina its regulation is essential for developing photoreceptor cells.

Pathways

IRX5 engages in critical developmental pathways including the Wnt and Hedgehog signaling pathways. It modulates these pathways by interacting with other proteins such as β-catenin in the Wnt pathway and Gli proteins in the Hedgehog pathway. The precise control of these signaling pathways by IRX5 is essential in maintaining the balance between growth and differentiation during development.

Associated diseases and disorders

IRX5 is associated with congenital heart defects and retinopathy. Mutations or dysregulation in IRX5 expression may lead to abnormalities in heart structure which are often linked with NKX2-5 another homeobox transcription factor involved in heart development. In the retina altered IRX5 function has connections to retinal degenerative conditions interacting with proteins such as CRX known for its role in photoreceptor function and survival. Understanding IRX5’s role in these diseases provides insights that might aid therapeutic development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-IRX5 antibody [EPR22724-43] (ab246494), expandable thumbnail

    Immunoprecipitation - Anti-IRX5 antibody [EPR22724-43] (ab246494)

    IRX5 was immunoprecipitated from 0.35 mg 293T transfected with a DDDDK-tagged mouse IRX5 (WT) expression vector, whole cell lysate 4 ug with ab246494 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246494 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: 293T transfected with a DDDDK-tagged mouse IRX5 (WT) expression vector, whole cell lysate 4 ug

    Lane 2: 293T transfected with a DDDDK-tagged mouse IRX5 (WT) expression vector, whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab246494 in 293T transfected with a DDDDK-tagged mouse IRX5 (WT) expression vector, whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second

    All lanes: Immunoprecipitation - Anti-IRX5 antibody [EPR22724-43] (ab246494)

    Predicted band size: 50 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-IRX5 antibody [EPR22724-43] (ab246494), expandable thumbnail

    Western blot - Anti-IRX5 antibody [EPR22724-43] (ab246494)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 3 seconds

    All lanes: Western blot - Anti-IRX5 antibody [EPR22724-43] (ab246494) at 1/1000 dilution

    Lane 1: HeLa transfected with a DDDDK-tagged mouse IRX3 (WT) expression vector, whole cell lysate at 4 µg

    Lane 2: HeLa transfected with a DDDDK-tagged mouse IRX5 (WT) expression vector, whole cell lysate at 4 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-IRX5 antibody [EPR22724-43] (ab246494), expandable thumbnail

    Western blot - Anti-IRX5 antibody [EPR22724-43] (ab246494)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 15 seconds

    The image is kindly provided by an anonymous collaborator.

    All lanes: Western blot - Anti-IRX5 antibody [EPR22724-43] (ab246494) at 1/1000 dilution

    Lane 1: IRX5 (WT) over expression whole cell lysate

    Lane 2: Mouse lung (WT) tissue lysate

    Lane 3: IRX5 knock out mouse lung tissue lysate

    Predicted band size: 50 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-IRX5 antibody [EPR22724-43] (ab246494), expandable thumbnail

    Western blot - Anti-IRX5 antibody [EPR22724-43] (ab246494)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    In lane 1, the lysate was stored at -80°C prior to Western Blotting. In lane 2, lysate was freshly made and used for Western Blotting immediately to minimize protein degradation.

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-IRX5 antibody [EPR22724-43] (ab246494) at 1/1000 dilution

    Lane 1: Mouse lung (frozen) tissue lysate at 20 µg

    Lane 2: Mouse lung (fresh) tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 50 kDa

    Observed band size: 60 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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