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AB235776

Anti-ISCA2 antibody

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(1 Publication)

Rabbit Polyclonal ISCA2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ISCA2.

View Alternative Names

HBLD1, ISCA2, HESB-like domain-containing protein 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISCA2 antibody (AB235776)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISCA2 antibody (AB235776)

Paraffin-embedded human tonsil tissue stained for ISCA2 using ab235776 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISCA2 antibody (AB235776)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISCA2 antibody (AB235776)

Paraffin-embedded human prostate cancer tissue stained for ISCA2 using ab235776 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-ISCA2 antibody (AB235776)
  • WB

Supplier Data

Western blot - Anti-ISCA2 antibody (AB235776)

All lanes:

Western blot - Anti-ISCA2 antibody (ab235776) at 1/1000 dilution

All lanes:

HL-60 (human promyelocytic leukemia cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ISCA2.

Q86U28

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

AB169540

Anti-Ndufs1 antibody [EPR11521(B)]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (AB169540)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ISCA2 also known as Iron-Sulfur Cluster Assembly 2 is a protein involved in the assembly of iron-sulfur clusters essential for various cellular processes. This protein has a molecular weight of approximately 14 kDa. ISCA2 is expressed in numerous tissues including the heart liver and skeletal muscles. It plays a fundamental role in the synthesis of these clusters which are integral for electron transfer and enzymatic reactions.
Biological function summary

The protein ISCA2 participates in the maturation of iron-sulfur cluster-containing proteins critical for mitochondrial function. As part of a larger complex ISCA2 collaborates with other proteins like ISCA1 and IBA57 to facilitate the transfer and insertion of these clusters into target proteins. This action supports the stability and functionality of key mitochondrial enzymes assisting in cellular respiration and redox homeostasis.

Pathways

Iron-sulfur cluster biogenesis involves ISCA2 and integrates into critical pathways like the mitochondrial respiratory chain and the citric acid cycle. Within these pathways ISCA2 operates alongside related proteins such as NFS1 and FXN ensuring proper cluster delivery and activity. These pathways are vital for maintaining cellular energy production and metabolic balance emphasizing ISCA2's significant role.

ISCA2 mutations have been implicated in mitochondrial diseases like multiple mitochondrial dysfunctions syndrome (MMDS). This disorder includes neurologic abnormalities due to defective mitochondrial protein function. ISCA2 is connected to MMDS alongside proteins such as ISCA1 and IBA57 which also partake in the cluster assembly machinery highlighting the protein's broader importance in human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway. May be involved in the binding of an intermediate of Fe/S cluster assembly.
See full target information ISCA2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:405 PubMed40393967

2025

Cellular crosstalk mediated by Meteorin-like regulating hepatic stellate cell activation during hepatic fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxia Zhou,Guifang Wang,Lingling Liu,Lingyu Song,Laying Hu,Lu Liu,Lifen Xu,Tuanlao Wang,Lirong Liu,Yuanyuan Wang,Tian Zhang,Bing Guo
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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