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AB154060

Anti-ISCU antibody

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(3 Publications)

Rabbit Polyclonal ISCU antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ISCU aa 1 to C-terminus.

View Alternative Names

NIFUN, ISCU, Iron-sulfur cluster assembly enzyme ISCU, NifU-like N-terminal domain-containing protein, NifU-like protein

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ISCU antibody (AB154060)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ISCU antibody (AB154060)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling ISCU protein at mitochondria using ab154060 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISCU antibody (AB154060)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISCU antibody (AB154060)

Immunohistochemical analysis of mouse muscle tissue stained for ISCU protein at cytosol with ab154060 at a 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISCU antibody (AB154060)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISCU antibody (AB154060)

Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labeling ISCU with ab154060 at 1/500 dilution.

Western blot - Anti-ISCU antibody (AB154060)
  • WB

Supplier Data

Western blot - Anti-ISCU antibody (AB154060)

Samples were separated by 15% SDS PAGE.

All lanes:

Western blot - Anti-ISCU antibody (ab154060) at 1/5000 dilution

Lane 1:

Non (-) transfected 293T cell lysates at 30 µg

Lane 2:

ISCU transfetected 293T cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ISCU aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H1K1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ISCU also known as the iron-sulfur cluster scaffold protein is an important player in the assembly of iron-sulfur clusters. This protein possesses a molecular mass of approximately 14 kDa and is widely expressed in mitochondria highlighting its central involvement in cellular processes dependent on these organelles. ISCU interacts with several proteins to facilitate the transfer of iron-sulfur clusters to apoproteins ensuring the proper maturation of these metalloproteins necessary for a variety of cellular functions.
Biological function summary

ISCU protein functions as an important scaffold component in the biosynthesis of iron-sulfur (Fe-S) clusters which are integral for the activity of multiple enzymatic and non-enzymatic systems. Within the mitochondria ISCU forms part of a multi-component complex that includes other scaffold proteins and necessary enzymes such as cysteine desulfurase which provides sulfur for cluster assembly. This complex orchestrates the precise coordination of metal ions and scaffold protein interactions facilitating cluster formation.

Pathways

The iron-sulfur cluster scaffold ISCU is vital in the electron transport chain and the citric acid cycle both of which are central to energy production in cells. ISCU closely interacts with proteins like frataxin which delivers iron to the scaffold and ferredoxin which is involved in electron transfer. These interactions highlight the pathway interdependence that maintains cellular bioenergetics and redox balance.

Disruptions in ISCU expression or function link to conditions such as Myopathy with exercise intolerance and Friedreich’s ataxia. Altered iron-sulfur cluster biogenesis impairs energy metabolism affecting muscle and neural tissues. ISCU's partnership with the frataxin protein becomes significant here as frataxin mutations result in similar pathological features thereby providing a potential target for therapeutic intervention in related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Mitochondrial scaffold protein, of the core iron-sulfur cluster (ISC) assembly complex, that provides the structural architecture on which the [2Fe-2S] clusters are assembled (PubMed : 34824239). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1 : LYRM4 : NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (Probable) (PubMed : 24971490, PubMed : 29576242, PubMed : 30031876, PubMed : 34824239). Exists as two slow interchanging conformational states, a structured (S) and disordered (D) form (PubMed : 23940031). May modulate NFS1 desulfurase activity in a zinc-dependent manner (PubMed : 30031876). Modulates the interaction between FXN and the cysteine desulfurase complex (PubMed : 29576242).. Isoform 2. Cytoplasmic scaffold protein, of the cytoplasmic core iron-sulfur cluster (ISC) assembly complex that provides the structural architecture on which the Fe-S clusters are assembled and may be involved in the cytoplasmic iron-sulfur protein biogenesis.
See full target information ISCU
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 21:e1011597 PubMed39908322

2025

Plant-specific cochaperone SSR1 affects root elongation by modulating the mitochondrial iron-sulfur cluster assembly machinery.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanjun Feng,Yue Hu,Tao Xie,Huiling Han,Diana Bonea,Lijuan Zeng,Jie Liu,Wenhan Ying,Bona Mu,Yuanyuan Cai,Min Zhang,Yanli Lu,Rongmin Zhao,Xuejun Hua

Anticancer research 44:4709-4721 PubMed39477289

2024

Genome-wide microRNA Analysis Identified Over-expression in Pancreatic Cancer Tissues as a Predictor of their Local Invasiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Tomohisa Otsu,Masamichi Hayashi,Keizo Fujita,Daigo Kobayashi,Nobuhiko Nakagawa,Keisuke Kurimoto,Hideki Takami,Koki Nakanishi,Shinichi Umeda,Dai Shimizu,Norifumi Hattori,Mitsuro Kanda,Chie Tanaka,Goro Nakayama,Yasuhiro Kodera

Aging 12:25229-25255 PubMed33223504

2020

Exposure to ozone impacted Th1/Th2 imbalance of CD T cells and apoptosis of ASMCs underlying asthmatic progression by activating lncRNA PVT1-miR-15a-5p/miR-29c-3p signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Wei,Baofen Han,Wenjuan Dai,Shufang Guo,Caiping Zhang,Lixuan Zhao,Yan Gao,Yi Jiang,Xiaomei Kong
View all publications
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