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AB176576

Anti-ISL-2 antibody [EPR12785]

3

(2 Reviews)

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(1 Publication)

Rabbit Recombinant Monoclonal ISL-2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Insulin gene enhancer protein ISL-2, Islet-2, ISL2

2 Images
Flow Cytometry (Intracellular) - Anti-ISL-2 antibody [EPR12785] (AB176576)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ISL-2 antibody [EPR12785] (AB176576)

Intracellular flow cytometric analysis of permeabilized 293 cells labeling Islet 2 with ab176576 at a 1/10 dilution (red), or a rabbit IgG negative control (green).

Western blot - Anti-ISL-2 antibody [EPR12785] (AB176576)
  • WB

Supplier Data

Western blot - Anti-ISL-2 antibody [EPR12785] (AB176576)

All lanes:

Western blot - Anti-ISL-2 antibody [EPR12785] (ab176576) at 1/1000 dilution

Lane 1:

HUVEC cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 40 kDa

true

  • Carrier free

    Anti-ISL-2 antibody [EPR12785] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12785

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Zebrafish": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ISL-2 also known as ISLET2 or Islet-2 is a transcription factor with an approximate mass of 39 kDa. It belongs to the LIM/homeobox family and primarily functions by binding DNA to regulate gene transcription. This protein is essential in the central nervous system and is also expressed in the developing spinal cord. In these tissues ISL-2 facilitates the differentiation and maturation of specific neuronal populations by influencing the transcription of downstream target genes.
Biological function summary

As part of a complex network of transcription factors ISL-2 plays an important role in neuronal development. It controls genes involved in motor neuron differentiation and axonal guidance. ISL-2 influences the identity and function of neurons participating in the formation of neural circuits. The protein's regulatory capacity ensures correct neuronal patterning impacting the functioning of developing and mature nervous systems.

Pathways

ISL-2 interacts with signaling pathways related to neuronal specification such as the Hedgehog and Wnt pathways. These pathways are important for the regulation of neural tube development and organization of neuronal structures. In the Wnt pathway ISL-2 interacts with proteins like β-catenin to modulate gene expression important for neuronal identity. Its involvement in these pathways reinforces the protein's function in maintaining nervous system integrity.

ISL-2 has implications in neurodegenerative diseases and spinal muscular atrophy. Mutations or dysregulation of ISL-2 expression can affect motor neuron health leading to progressive loss of motor function. It shows connections with other proteins involved in neurodegeneration such as SMN (survival motor neuron protein) which plays a role in the disease's pathology. Understanding ISL-2's regulation and interactions can offer insights into therapeutic strategies for such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.
See full target information ISL2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:752 PubMed39419978

2024

PAK6 rescues pathogenic LRRK2-mediated ciliogenesis and centrosomal cohesion defects in a mutation-specific manner.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Iannotta,Rachel Fasiczka,Giulia Favetta,Yibo Zhao,Elena Giusto,Elena Dall'Ara,Jianning Wei,Franz Y Ho,Claudia Ciriani,Susanna Cogo,Isabella Tessari,Ciro Iaccarino,Maxime Liberelle,Luigi Bubacco,Jean-Marc Taymans,Claudia Manzoni,Arjan Kortholt,Laura Civiero,Sabine Hilfiker,Michael L Lu,Elisa Greggio
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com