Anti-Islet 1 antibody [1H9] (ab86472) is a mouse monoclonal antibody that is used to detect Islet 1 in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, samples.
- Specificity confirmed with Islet 1 knockout cell line validation
Preservative: 0.05% Sodium azide
Constituents: PBS
Flow Cyt | WB | IHC-P | ICC/IF | |
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Human | Tested | Tested | Tested | Tested |
Transfected cell lysate - Human | Not recommended | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
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Species Human | Dilution info 1/50 - 1/100 | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
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Species Transfected cell lysate - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Transfected cell lysate - Human | Dilution info 1/500 - 1/2000 | Notes - |
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info 1/200 - 1/1000 | Notes - |
Species | Dilution info | Notes |
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Species Transfected cell lysate - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info 1/200 - 1/1000 | Notes - |
Species | Dilution info | Notes |
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Species Transfected cell lysate - Human | Dilution info - | Notes - |
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DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1 (By similarity). Binds to insulin gene enhancer sequences (By similarity). Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart (By similarity).
Insulin gene enhancer protein ISL-1, Islet-1, ISL1
Anti-Islet 1 antibody [1H9] (ab86472) is a mouse monoclonal antibody that is used to detect Islet 1 in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, samples.
- Specificity confirmed with Islet 1 knockout cell line validation
Preservative: 0.05% Sodium azide
Constituents: PBS
In western blot, we observe a specific band at ~40kDa which is not seen in KO cell lines. A cross-reactive band was observed in the wild-type and knockout cells. The additional band below this band of interest is seen at ~38kDa in both the WT and KO cells. We are unsure as to the identity of these extra bands.
Purified from tissue culture supernatant.
This product was changed from ascites to supernatant. Lot no's high than GR141006-29 are from Tissue Culture Supernatant
Islet 1 also known as ISL1 is a LIM-homeodomain transcription factor with a molecular mass of approximately 38 kDa. It plays an important role in regulating gene expression during the development of the nervous system and pancreas. You can find Islet 1 predominantly in motor neurons pancreatic beta cells and cardiac muscle cells. Its expression is important during embryonic stages for cell differentiation and maturation. Researchers use "Isl 1 neuronal marker" antibodies to identify this protein's presence in tissue.
Islet 1 is involved in the differentiation and survival of various cell types. As part of a transcription complex it regulates downstream target genes essential for the development of motor neurons and cardiac muscle. It also contributes significantly to the developmental pathways of insulin-producing beta cells in the pancreas. The ISL1 marker is therefore an important tool for studying cell lineage and fate in developmental biology.
Islet 1 is involved in key developmental signaling pathways including the Notch signaling and Wnt signaling pathways. In these pathways Islet 1 collaborates with other proteins like Lhx3 and Ngn2 to ensure proper gene activation for neuronal and cardiac development. Wnt signaling affects the expression of isl 1 marker genes contributing to precise spatial and temporal regulation during embryogenesis.
Islet 1 has links to congenital heart defects and type 2 diabetes. Mutations or altered expression of this protein can disrupt pancreatic beta cell function and motor neuron development. In congenital heart disease the interaction of Islet 1 with proteins like Nkx2.5 is critical. Its role in type 2 diabetes ties with the functioning of other proteins involved in insulin secretion making it a potential target for therapeutic intervention in these conditions.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Islet 1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Islet 1 antibody
ab86472 at a 1/200 dilution staining Islet 1 in human lung cancer tissue. DAB staining shows nuclear localisation.
Islet 1 Western blot staining using mouse Anti-Islet 1 antibody
ab86472 was shown to recognize Islet 1 in wild-type HAP1 cells as signal was lost at the expected MW in Islet 1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Islet 1 knockout samples were subjected to SDS-PAGE. ab86472 and Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472) at 1/500 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: ISL1 (Islet 1) knockout HAP1 whole cell lysate at 20 µg
Lane 3: SH-SY5Y whole cell lysate at 20 µg
Lane 4: HepG2 whole cell lysate at 20 µg
Predicted band size: 39 kDa
Observed band size: 40 kDa
Islet 1 Western blot staining of HEK293 cells transfected with full length Islet 1 (aa1-349)-hIgGFc using mouse Anti-Islet 1 antibody
Note: The molecular weight is larger than expected, as the recombinant protein is not the endogenouse protein, but rather the fc-fusion protein
All lanes: Western blot - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472) at 1/500 dilution
All lanes: HEK293 cells transfected with full length Islet 1 (aa1-349)-hIgGFc
Predicted band size: 39 kDa
Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length Islet 1-hIgGFc using ab86472 at 1/150 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
Islet 1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Islet 1 antibody
ab86472 at a 1/200 dilution staining Islet 1 in human cervical carcinoma tissue. DAB staining shows nuclear localization.
Islet 1 Flow Cytometry staining using mouse Anti-Islet 1 antibody
Overlay histogram showing SH-SY5Y cells stained with ab86472 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86472, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.
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