Anti-Islet 1 antibody [1H9] (ab86472) is a mouse monoclonal antibody that is used to detect Islet 1 in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, samples.
- Specificity confirmed with Islet 1 knockout cell line validation
Images
![Western blot - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (AB86472), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-1h9-neural-stem-cell-marker-ab86472--western-blot-img50378.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (AB86472)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (AB86472), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-1h9-neural-stem-cell-marker-ab86472--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img94925.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (AB86472)")
![Western blot - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (AB86472), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-1h9-neural-stem-cell-marker-ab86472--western-blot-img142122.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (AB86472)")
![Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (AB86472), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-1h9-neural-stem-cell-marker-ab86472--immunocytochemistry-immunofluorescence-img52704.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (AB86472)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (AB86472), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-1h9-neural-stem-cell-marker-ab86472--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101236.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (AB86472)")
Publications
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- Cell proliferation 56:e134922023An aggregation of human embryonic and trophoblast stem cells reveals the role of trophectoderm on epiblast differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXulun Wu,Wentao Zhao,Hao Wu,Qiancheng Zhang,Yiming Wang,Kunyuan Yu,Jinglei Zhai,Fan Mo,Meijiao Wang,Shiwen Li,Xili Zhu,Xiaoyan Liang,Baoyang Hu,Guang-Hui Liu,Jun Wu,Hongmei Wang,Fan Guo,Leqian YuPubMed 37199067
- The Journal of clinical endocrinology and metabolism 107:e4154-e41582022Early Detection of Relapse by ctDNA Sequencing in a Patient with Metastatic Thymic Tumor and MEN1 Mosaicism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArnaud Lagarde,Lauriane Le Collen,Camille Boulagnon,Hedia Brixi,Anne Durlach,Grégory Mougel,Thomas Cuny,Brigitte Delemer,Anne Barlier,Pauline RomanetPubMed 35904487
- Frontiers in integrative neuroscience 16:8550712022Validation of Functional Connectivity of Engineered Neuromuscular Junction With Recombinant Monosynaptic Pseudotyped ΔG-Rabies Virus Tracing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUlrich Stefan Bauer,Vegard Fiskum,Rajeevkumar Raveendran Nair,Rosanne van de Wijdeven,Clifford Kentros,Ioanna Sandvig,Axel SandvigPubMed 35669734
- Scientific reports 11:217222021Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNarges Mahmoodi,Jafar Ai,Zahra Hassannejad,Somayeh Ebrahimi-Barough,Elham Hasanzadeh,Houra Nekounam,Alexander R Vaccaro,Vafa Rahimi-MovagharPubMed 34741076
- Nature communications 12:33592021TET1-mediated DNA hydroxymethylation regulates adult remyelination in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Moyon,Rebecca Frawley,Damien Marechal,Dennis Huang,Katy L H Marshall-Phelps,Linde Kegel,Sunniva M K Bøstrand,Boguslawa Sadowski,Yong-Hui Jiang,David A Lyons,Wiebke Möbius,Patrizia CasacciaPubMed 34099715
- Cell proliferation 54:e130412021Therapeutic effects of CXCR4 subpopulation of transgene-free induced cardiosphere-derived cells on experimental myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianyong Xu,Huimei Wu,Zhigang Mai,Junbo Yi,Xianqi Wang,Lingyun Li,Zhong HuangPubMed 33942933
- The Malaysian journal of medical sciences : MJMS 27:37-452020The Role of ISL1 and LHX5 LIM Homeobox Genes in Bladder Tumourigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohd Khairul Anuar Md Akhir et. al.PubMed 32158343
- Cell biology international 44:1168-11832020Microtubule stabilizer epothilone B as a motor neuron differentiation agent for human endometrial stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNarges Mahmoodi,Jafar Ai,Somayeh Ebrahimi-Barough,Zahra Hassannejad,Elham Hasanzadeh,Arefeh Basiri,Alexander R Vaccaro,Vafa Rahimi-MovagharPubMed 32022385
- International journal of molecular sciences 20:2019Effects of Spaceflight and Simulated Microgravity on YAP1 Expression in Cardiovascular Progenitors: Implications for Cell-Based Repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVictor Camberos et. al.PubMed 31167392
- International journal of molecular sciences 20:2019Differentiation of Motor Neuron-Like Cells from Tonsil-Derived Mesenchymal Stem Cells and Their Possible Application to Neuromuscular Junction Formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaeyoung Park et. al.PubMed 31159418
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human ISL1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P61371
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Transfected cell lysate - Human | Not recommended | Tested | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/500 - 1/2000 | Notes - |
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1 (By similarity). Binds to insulin gene enhancer sequences (By similarity). Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart (By similarity).
Alternative names
Insulin gene enhancer protein ISL-1, Islet-1, ISL1
Recommended products
Anti-Islet 1 antibody [1H9] (ab86472) is a mouse monoclonal antibody that is used to detect Islet 1 in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, samples.
- Specificity confirmed with Islet 1 knockout cell line validation
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human ISL1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P61371
- Clone number
- 1H9
- Purification technique
- Affinity purification Protein G
- Specificity
In western blot, we observe a specific band at ~40kDa which is not seen in KO cell lines. A cross-reactive band was observed in the wild-type and knockout cells. The additional band below this band of interest is seen at ~38kDa in both the WT and KO cells. We are unsure as to the identity of these extra bands.
- Concentration
- Purification notes
Purified from tissue culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Islet 1 antibody [1H9] (ab86472) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, samples.
What is the molecular weight of Islet 1?
Anti-Islet 1 [1H9] (ab86472) specifically detects a band for Islet 1 (UniProt: P61371) at a molecular weight of 39kDa.
Trusted by the scientific community
Anti-Islet 1 [1H9] (ab86472) was first used in a scientific publication in 2009 and has been cited over 10 times in peer-reviewed journals.
Specificity confirmed
The specificity of Anti-Islet 1 antibody [1H9] (ab86472) has been confirmed by Western blot testing in ISL1 Knockout HAP1 cells.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Islet 1 also known as ISL1 is a LIM-homeodomain transcription factor with a molecular mass of approximately 38 kDa. It plays an important role in regulating gene expression during the development of the nervous system and pancreas. You can find Islet 1 predominantly in motor neurons pancreatic beta cells and cardiac muscle cells. Its expression is important during embryonic stages for cell differentiation and maturation. Researchers use "Isl 1 neuronal marker" antibodies to identify this protein's presence in tissue.
- Biological function summary
Islet 1 is involved in the differentiation and survival of various cell types. As part of a transcription complex it regulates downstream target genes essential for the development of motor neurons and cardiac muscle. It also contributes significantly to the developmental pathways of insulin-producing beta cells in the pancreas. The ISL1 marker is therefore an important tool for studying cell lineage and fate in developmental biology.
- Pathways
Islet 1 is involved in key developmental signaling pathways including the Notch signaling and Wnt signaling pathways. In these pathways Islet 1 collaborates with other proteins like Lhx3 and Ngn2 to ensure proper gene activation for neuronal and cardiac development. Wnt signaling affects the expression of isl 1 marker genes contributing to precise spatial and temporal regulation during embryogenesis.
- Associated diseases and disorders
Islet 1 has links to congenital heart defects and type 2 diabetes. Mutations or altered expression of this protein can disrupt pancreatic beta cell function and motor neuron development. In congenital heart disease the interaction of Islet 1 with proteins like Nkx2.5 is critical. Its role in type 2 diabetes ties with the functioning of other proteins involved in insulin secretion making it a potential target for therapeutic intervention in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
![Western blot - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-1h9-neural-stem-cell-marker-ab86472--western-blot-img50378.jpg "Western blot - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472)")
Western blot - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472)
Islet 1 Western blot staining using mouse Anti-Islet 1 antibody
Lanes 1 - 4: Merged signal (red and green). Green - ab86472 observed at 40 kDa. Red - loading control, Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560, observed at 50 kDa.ab86472 was shown to recognize Islet 1 in wild-type HAP1 cells as signal was lost at the expected MW in Islet 1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Islet 1 knockout samples were subjected to SDS-PAGE. ab86472 and Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472) at 1/500 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: ISL1 (Islet 1) knockout HAP1 whole cell lysate at 20 µg
Lane 3: SH-SY5Y whole cell lysate at 20 µg
Lane 4: HepG2 whole cell lysate at 20 µg
Predicted band size: 39 kDa
Observed band size: 40 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-1h9-neural-stem-cell-marker-ab86472--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img94925.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472)
Islet 1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Islet 1 antibody
ab86472 at a 1/200 dilution staining Islet 1 in human lung cancer tissue. DAB staining shows nuclear localisation.
![Western blot - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-1h9-neural-stem-cell-marker-ab86472--western-blot-img142122.jpg "Western blot - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472)")
Western blot - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472)
Islet 1 Western blot staining of HEK293 cells transfected with full length Islet 1 (aa1-349)-hIgGFc using mouse Anti-Islet 1 antibody
Note: The molecular weight is larger than expected, as the recombinant protein is not the endogenouse protein, but rather the fc-fusion protein
All lanes: Western blot - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472) at 1/500 dilution
All lanes: HEK293 cells transfected with full length Islet 1 (aa1-349)-hIgGFc
Predicted band size: 39 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-1h9-neural-stem-cell-marker-ab86472--immunocytochemistry-immunofluorescence-img52704.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472)")
Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472)
Islet 1 Immunocytochemistry/ Immunofluorescence staining using mouse Anti-Islet 1 antibody
Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length Islet 1-hIgGFc using ab86472 at 1/150 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-1h9-neural-stem-cell-marker-ab86472--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101236.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472)
Islet 1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Islet 1 antibody
ab86472 at a 1/200 dilution staining Islet 1 in human cervical carcinoma tissue. DAB staining shows nuclear localization.
![Flow Cytometry - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-1h9-neural-stem-cell-marker-ab86472--flow-cytometry-img414267.jpg "Flow Cytometry - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472)")
Flow Cytometry - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472)
Islet 1 Flow Cytometry staining using mouse Anti-Islet 1 antibody
Overlay histogram showing SH-SY5Y cells stained with ab86472 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86472, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.
![Flow Cytometry - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-1h9-neural-stem-cell-marker-ab86472--flow-cytometry-img414267.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Islet 1 antibody [1H9] - Neural Stem Cell Marker (ab86472)")
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