Anti-Islet 1 antibody [EP4182] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (AB216657)

Intracellular Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Islet 1 with purified ab109517 at 1/50 dilution (10 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109517).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (AB216657)

Immunocytochemistry/Immunofluorescence analysis of SH-SY-5Y (human neuroblastoma) cells labelling Islet 1 with purified ab109517 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109517).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (AB216657)

Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Islet 1 with unpurified ab109517.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109517).

• IP

Unknown

Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (AB216657)

ab109517 (purified) at 1/30 immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. 10 μg of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) was used for detection at 1 : 1500 dilution. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109517).

All lanes:

Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (<a href='/en-us/products/primary-antibodies/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517'>ab109517</a>)

Predicted band size: 39 kDa

false

• IP

Lab

Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (AB216657)

ab109517 (unpurified) immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109517).

All lanes:

Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (<a href='/en-us/products/primary-antibodies/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517'>ab109517</a>)

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Exposure time: 30s