Rabbit Recombinant Monoclonal Islet 1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Chicken samples. Cited in 3 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (AB216657), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-bsa-and-azide-free-ab216657--immunocytochemistry-immunofluorescence-img19686.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (AB216657)")
![Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (AB216657), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-bsa-and-azide-free-ab216657--immunocytochemistry-immunofluorescence-img117061.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (AB216657)")
![Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (AB216657), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-bsa-and-azide-free-ab216657--immunoprecipitation-img648.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (AB216657)")
![Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (AB216657), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-bsa-and-azide-free-ab216657--flow-cytometry-intracellular-img2774.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (AB216657)")
![Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (AB216657), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-bsa-and-azide-free-ab216657--immunoprecipitation-img11746.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (AB216657)")
Publications
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- Proceedings of the National Academy of Sciences of 112:E1861-702015Islet 1 specifies the identity of hypothalamic melanocortin neurons and is critical for normal food intake and adiposity in adulthood.
- Development (Cambridge, England) 141:4690-62014Embryonic maturation of epidermal Merkel cells is controlled by a redundant transcription factor network.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarolina N Perdigoto,Evan S Bardot,Victor J Valdes,Francis J Santoriello,Elena EzhkovaPubMed 25468937
- Molecular cancer 13:1812014ISL-1 is overexpressed in non-Hodgkin lymphoma and promotes lymphoma cell proliferation by forming a p-STAT3/p-c-Jun/ISL-1 complex.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Chicken | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
7 products for Alternative Version
Target data
Function
DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1 (By similarity). Binds to insulin gene enhancer sequences (By similarity). Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart (By similarity).
Alternative names
Insulin gene enhancer protein ISL-1, Islet-1, ISL1
Recommended products
Rabbit Recombinant Monoclonal Islet 1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Chicken samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP4182
- Purification technique
- Affinity purification Protein A
- Specificity
86% identities with Islet 2
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab216657 is the carrier-free version of Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab109517.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Islet 1 also known as ISL1 is a LIM-homeodomain transcription factor with a molecular mass of approximately 38 kDa. It plays an important role in regulating gene expression during the development of the nervous system and pancreas. You can find Islet 1 predominantly in motor neurons pancreatic beta cells and cardiac muscle cells. Its expression is important during embryonic stages for cell differentiation and maturation. Researchers use "Isl 1 neuronal marker" antibodies to identify this protein's presence in tissue.
- Biological function summary
Islet 1 is involved in the differentiation and survival of various cell types. As part of a transcription complex it regulates downstream target genes essential for the development of motor neurons and cardiac muscle. It also contributes significantly to the developmental pathways of insulin-producing beta cells in the pancreas. The ISL1 marker is therefore an important tool for studying cell lineage and fate in developmental biology.
- Pathways
Islet 1 is involved in key developmental signaling pathways including the Notch signaling and Wnt signaling pathways. In these pathways Islet 1 collaborates with other proteins like Lhx3 and Ngn2 to ensure proper gene activation for neuronal and cardiac development. Wnt signaling affects the expression of isl 1 marker genes contributing to precise spatial and temporal regulation during embryogenesis.
- Associated diseases and disorders
Islet 1 has links to congenital heart defects and type 2 diabetes. Mutations or altered expression of this protein can disrupt pancreatic beta cell function and motor neuron development. In congenital heart disease the interaction of Islet 1 with proteins like Nkx2.5 is critical. Its role in type 2 diabetes ties with the functioning of other proteins involved in insulin secretion making it a potential target for therapeutic intervention in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-bsa-and-azide-free-ab216657--immunocytochemistry-immunofluorescence-img19686.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)")
Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)
Immunocytochemistry/Immunofluorescence analysis of SH-SY-5Y (human neuroblastoma) cells labelling Islet 1 with purified Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab109517 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab109517).
![Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-bsa-and-azide-free-ab216657--immunocytochemistry-immunofluorescence-img117061.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)")
Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)
Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Islet 1 with unpurified Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab109517.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab109517).
![Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-bsa-and-azide-free-ab216657--immunoprecipitation-img648.jpg "Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)")
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab109517 (purified) at 1/30 immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. 10 μg of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1500 dilution. A rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used intead of Anti-Rab11A antibody [EPR7587(B)] ab128913 as a negative control (Lane 3).
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab109517).
All lanes: Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab109517)
Predicted band size: 39 kDa
![Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-bsa-and-azide-free-ab216657--flow-cytometry-intracellular-img2774.jpg "Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)")
Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)
Intracellular Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Islet 1 with purified Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab109517 at 1/50 dilution (10 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab109517).
![Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-bsa-and-azide-free-ab216657--immunoprecipitation-img11746.jpg "Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)")
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab109517 (unpurified) immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab109517).
All lanes: Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab109517)
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 30s
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