Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
- RabMAb
- Recombinant
- Lab Essentials
- 20ul selling size
- What is this?
5
(6 Reviews)
|
(77 Publications)
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) is a rabbit monoclonal antibody detecting Islet 1 in Western Blot, Flow Cytometry (Intra), IP, ICC/IF. Suitable for Chicken, Human, Mouse.
- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications
View Alternative Names
Insulin gene enhancer protein ISL-1, Islet-1, ISL1
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
Immunocytochemistry/Immunofluorescence analysis of SH-SY-5Y (human neuroblastoma) cells labelling Islet 1 with purified ab109517 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
- Flow Cyt (Intra)
Lab
Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
Intracellular Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Islet 1 with purified ab109517 at 1/50 dilution (10μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Islet 1 with unpurified ab109517.
- IP
Unknown
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
ab109517 (purified) at 1/30 immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. 10 μg of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking/Dilution buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
Predicted band size: 39 kDa
false
- IP
Lab
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
ab109517 (unpurified) immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate.
All lanes:
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
Predicted band size: 39 kDa
Observed band size: 39 kDa
false
Exposure time: 30s
- WB
Unknown
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
Blocking/Dilution buffer and concentration : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution
All lanes:
HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 39 kDa
false
- WB
Unknown
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
Blocking/Dilution buffer and concentration : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution
All lanes:
K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 39 kDa
false
- WB
Lab
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Lane 1, 2 : 10 seconds Lane 3 : 3 seconds
All lanes:
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/1000 dilution
Lanes 1 - 3:
SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lane 2:
Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 40 kDa,36 kDa
false
- WB
Unknown
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
All lanes:
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution
Lane 1:
K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 10 µg
Lane 2:
HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
Lane 3:
Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg
Lane 4:
SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg
Predicted band size: 39 kDa
false
- WB
Unknown
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
Blocking/Dilution buffer and concentration : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution
All lanes:
RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 39 kDa,54 kDa
Observed band size: 80 kDa
false
- WB
Unknown
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.
All lanes:
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution
All lanes:
UMNSAH/DF-1 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 39 kDa
false
Related conjugates and formulations (8)
-
Anti-Islet 1 antibody [EP4182] - BSA and Azide free
-
660 APC
APC Anti-Islet 1antibody [EP4182] - Neural Stem Cell Marker
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
-
603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
-
578 PE
PE Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
Reactivity data
Product details
Insulin gene enhancer protein ISL-1 is a protein encoded by the ISL1 gene. Among other roles, ISL-1 plays a part in the embryogenesis of pancreatic islets of Langerhans and some models, deficiency have shown failure of neural tube motor neurons to differentiate.
Rat: We have preliminary testing data to indicate this antibody may not react with these species. Please contact us for more information.
Product Specifications
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IP, WB in chicken, human, mouse samples.
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) specifically detects Islet 1 (UniProt ID: P61371; Molecular weight: 39kDa) and is sold in 100 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) has been cited over 59 times in peer reviewed journals and is trusted by the scientific community.
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) has 6 independent reviews from customers.
Related Products
Antibody clone EP4182 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 594, Alexa Fluor® 555, Alexa Fluor® 568, Alexa Fluor® 488, Alexa Fluor® 647, APC, PE (ab203404, ab203405, ab203406, ab275990, ab277925, ab310815, ab310894).
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Islet 1 is involved in the differentiation and survival of various cell types. As part of a transcription complex it regulates downstream target genes essential for the development of motor neurons and cardiac muscle. It also contributes significantly to the developmental pathways of insulin-producing beta cells in the pancreas. The ISL1 marker is therefore an important tool for studying cell lineage and fate in developmental biology.
Pathways
Islet 1 is involved in key developmental signaling pathways including the Notch signaling and Wnt signaling pathways. In these pathways Islet 1 collaborates with other proteins like Lhx3 and Ngn2 to ensure proper gene activation for neuronal and cardiac development. Wnt signaling affects the expression of isl 1 marker genes contributing to precise spatial and temporal regulation during embryogenesis.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (77)
Recent publications for all applications. Explore the full list and refine your search
eLife 13: PubMed40704991
2025
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Scientific reports 15:12516 PubMed40216954
2025
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Current protocols 5:e70091 PubMed39781605
2025
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Scientific reports 14:31182 PubMed39732807
2024
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Stem cell research & therapy 15:465 PubMed39639398
2024
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Scientific reports 14:25166 PubMed39448766
2024
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Cell reports. Medicine 5:101659 PubMed39067446
2024
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Molecular therapy : the journal of the American Society of Gene Therapy 32:2584-2603 PubMed38845196
2024
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Genome biology 25:140 PubMed38807229
2024
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Nature cardiovascular research 2:1060-1077 PubMed38524149
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com