Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) is a rabbit monoclonal antibody detecting Islet 1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, ICC/IF. Suitable for Chicken, Human, Mouse.
- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--immunocytochemistry-immunofluorescence-img19686.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)")
![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--western-blot-img115379.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)")
![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--western-blot-img86793.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)")
![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--western-blot-img53368.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)")
![Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--immunocytochemistry-immunofluorescence-img117061.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)")
Publications
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- iScience 26:1083062023Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolong Zheng,Zhixian Liu,Ziyu He,Jia Xu,YaNan Wang,ChenZi Gong,Ruoying Zhang,Su-Chun Zhang,Hong Chen,Wei WangPubMed 38026209
- iScience 26:1081522023FUS-dependent microRNA deregulations identify TRIB2 as a druggable target for ALS motor neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWan Yun Ho,Li-Ling Chak,Jin-Hui Hor,Fujia Liu,Sandra Diaz-Garcia,Jer-Cherng Chang,Emma Sanford,Maria J Rodriguez,Durgadevi Alagappan,Su Min Lim,Yik-Lam Cho,Yuji Shimizu,Alfred Xuyang Sun,Sheue-Houy Tyan,Edward Koo,Seung Hyun Kim,John Ravits,Shi-Yan Ng,Katsutomo Okamura,Shuo-Chien LingPubMed 37920668
- iScience 26:1067012023Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Han H Hsu,Greta Pintacuda,Ruize Liu,Eugeniu Nacu,April Kim,Kalliopi Tsafou,Natalie Petrossian,William Crotty,Jung Min Suh,Jackson Riseman,Jacqueline M Martin,Julia C Biagini,Daya Mena,Joshua K T Ching,Edyta Malolepsza,Taibo Li,Tarjinder Singh,Tian Ge,Shawn B Egri,Benjamin Tanenbaum,Caroline R Stanclift,Annie M Apffel,Steven A Carr,Monica Schenone,Jake Jaffe,Nadine Fornelos,Hailiang Huang,Kevin C Eggan,Kasper LagePubMed 37207277
- Cell genomics 3:1002502023Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGreta Pintacuda,Yu-Han H Hsu,Kalliopi Tsafou,Ka Wan Li,Jacqueline M Martín,Jackson Riseman,Julia C Biagini,Joshua K T Ching,Daya Mena,Miguel A Gonzalez-Lozano,Shawn B Egri,Jake Jaffe,August B Smit,Nadine Fornelos,Kevin C Eggan,Kasper LagePubMed 36950384
- Aging 14:9832-98592022Transcriptomic analysis of human ALS skeletal muscle reveals a disease-specific pattern of dysregulated circRNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDimitrios Tsitsipatis,Krystyna Mazan-Mamczarz,Ying Si,Allison B Herman,Jen-Hao Yang,Abhishek Guha,Yulan Piao,Jinshui Fan,Jennifer L Martindale,Rachel Munk,Xiaoling Yang,Supriyo De,Brijesh K Singh,Ritchie Ho,Myriam Gorospe,Peter H KingPubMed 36585921
- Stem cells international 2022:13209502022The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCatherine Schaefers,Simone Rothmiller,Horst Thiermann,Theo Rein,Annette SchmidtPubMed 36530489
- Cell reports methods 2:1003522022De-erosion of X chromosome dosage compensation by the editing of regulatory regions restores the differentiation potential in hPSCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNami Motosugi,Akiko Sugiyama,Chisa Okada,Asako Otomo,Akihiro Umezawa,Hidenori Akutsu,Shinji Hadano,Atsushi FukudaPubMed 36590687
- Current protocols 2:e5682022Dynamic 3D Combinatorial Generation of hPSC-Derived Neuromesodermal Organoids With Diverse Regional and Cellular Identities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDosh Whye,Delaney Wood,Kristina H Kim,Cidi Chen,Nina Makhortova,Mustafa Sahin,Elizabeth D ButtermorePubMed 36264199
- eLife 11:2022Airway basal cells show regionally distinct potential to undergo metaplastic differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYizhuo Zhou,Ying Yang,Lihao Guo,Jun Qian,Jian Ge,Debora Sinner,Hongxu Ding,Andrea Califano,Wellington V CardosoPubMed 36178196
- Nature protocols 17:2699-27192022Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeishi Kishimoto,Kentaro Iwasawa,Alice Sorel,Carlos Ferran-Heredia,Lu Han,Mitsuru Morimoto,James M Wells,Takanori Takebe,Aaron M ZornPubMed 35978039
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Chicken | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10000 - 1/50000 | Notes - |
Species Chicken | Dilution info 1/10000 - 1/50000 | Notes - |
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes For unpurified use at 1/50 - 1/100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
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Target data
Function
DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1 (By similarity). Binds to insulin gene enhancer sequences (By similarity). Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart (By similarity).
Alternative names
Insulin gene enhancer protein ISL-1, Islet-1, ISL1
Recommended products
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) is a rabbit monoclonal antibody detecting Islet 1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, ICC/IF. Suitable for Chicken, Human, Mouse.
- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP4182
- Purification technique
- Affinity purification Protein A
- Specificity
86% identities with Islet 2
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Insulin gene enhancer protein ISL-1 is a protein encoded by the ISL1 gene. Among other roles, ISL-1 plays a part in the embryogenesis of pancreatic islets of Langerhans and some models, deficiency have shown failure of neural tube motor neurons to differentiate.
Rat: We have preliminary testing data to indicate this antibody may not react with these species. Please contact us for more information.
Product SpecificationsAnti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IP, WB in chicken, human, mouse samples.
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) specifically detects Islet 1 (UniProt ID: P61371; Molecular weight: 39kDa) and is sold in 100 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) has been cited over 59 times in peer reviewed journals and is trusted by the scientific community.
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) has 6 independent reviews from customers.
Related Products
Antibody clone EP4182 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 594, Alexa Fluor® 555, Alexa Fluor® 568, Alexa Fluor® 488, Alexa Fluor® 647, APC, PE (Alexa Fluor® 594 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab203404, Alexa Fluor® 555 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab203405, Alexa Fluor® 568 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab203406, Alexa Fluor® 488 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab275990, Alexa Fluor® 647 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab277925, APC Anti-Islet 1antibody [EP4182] - Neural Stem Cell Marker ab310815, PE Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker ab310894).
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--immunocytochemistry-immunofluorescence-img19686.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
Immunocytochemistry/Immunofluorescence analysis of SH-SY-5Y (human neuroblastoma) cells labelling Islet 1 with purified ab109517 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--western-blot-img115379.jpg "Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
All lanes: Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution
Lane 1: K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 10 µg
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg
Lane 4: SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg
Predicted band size: 39 kDa
![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--western-blot-img86793.jpg "Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.All lanes: Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution
All lanes: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 39 kDa, 54 kDa
Observed band size: 80 kDa
![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--western-blot-img53368.jpg "Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.All lanes: Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution
All lanes: UMNSAH/DF-1 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 39 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--immunocytochemistry-immunofluorescence-img117061.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Islet 1 with unpurified ab109517.
![Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--immunoprecipitation-img648.jpg "Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
ab109517 (purified) at 1/30 immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. 10 μg of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used intead of Anti-Rab11A antibody [EPR7587(B)] ab128913 as a negative control (Lane 3).
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
Predicted band size: 39 kDa
![Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--flow-cytometry-intracellular-img2774.jpg "Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
Intracellular Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Islet 1 with purified ab109517 at 1/50 dilution (10μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
![Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--immunoprecipitation-img11746.jpg "Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
ab109517 (unpurified) immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate.
All lanes: Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 30s
![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--western-blot-img164232.jpg "Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution
All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 39 kDa
![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--western-blot-img86580.jpg "Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution
All lanes: K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 39 kDa
![Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--immunoprecipitation-img648.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
![Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--flow-cytometry-intracellular-img2774.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
![Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--immunoprecipitation-img11746.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--western-blot-img164232.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517), expandable thumbnail](https://content.abcam.com/products/images/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517--western-blot-img86580.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
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