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AB109517

Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

  • RabMAb
  • Recombinant
  • Lab Essentials
  • 20ul selling size
  • What is this?

5

(6 Reviews)

|

(77 Publications)

Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) is a rabbit monoclonal antibody detecting Islet 1 in Western Blot, Flow Cytometry (Intra), IP, ICC/IF. Suitable for Chicken, Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications

View Alternative Names

Insulin gene enhancer protein ISL-1, Islet-1, ISL1

11 Images
Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)

Immunocytochemistry/Immunofluorescence analysis of SH-SY-5Y (human neuroblastoma) cells labelling Islet 1 with purified ab109517 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)

Intracellular Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Islet 1 with purified ab109517 at 1/50 dilution (10μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)

Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Islet 1 with unpurified ab109517.

Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
  • IP

Unknown

Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)

ab109517 (purified) at 1/30 immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. 10 μg of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Predicted band size: 39 kDa

false

Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
  • IP

Lab

Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)

ab109517 (unpurified) immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate.

All lanes:

Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Exposure time: 30s

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
  • WB

Unknown

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 39 kDa

false

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
  • WB

Unknown

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution

All lanes:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 39 kDa

false

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
  • WB

Lab

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lane 1, 2 : 10 seconds Lane 3 : 3 seconds

All lanes:

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/1000 dilution

Lanes 1 - 3:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 40 kDa,36 kDa

false

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
  • WB

Unknown

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)

All lanes:

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 10 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg

Lane 4:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg

Predicted band size: 39 kDa

false

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
  • WB

Unknown

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution

All lanes:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 39 kDa,54 kDa

Observed band size: 80 kDa

false

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)
  • WB

Unknown

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB109517)

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution

All lanes:

UMNSAH/DF-1 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 39 kDa

false

  • Carrier free

    Anti-Islet 1 antibody [EP4182] - BSA and Azide free

  • 660 APC

    APC Anti-Islet 1antibody [EP4182] - Neural Stem Cell Marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

  • 578 PE

    PE Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP4182

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Chicken, Human

Applications

Flow Cyt (Intra), IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

86% identities with Islet 2

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/50 - 1/100.</strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chicken": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

Product details

Insulin gene enhancer protein ISL-1 is a protein encoded by the ISL1 gene. Among other roles, ISL-1 plays a part in the embryogenesis of pancreatic islets of Langerhans and some models, deficiency have shown failure of neural tube motor neurons to differentiate.

Rat: We have preliminary testing data to indicate this antibody may not react with these species. Please contact us for more information.

Product Specifications
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IP, WB in chicken, human, mouse samples.
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) specifically detects Islet 1 (UniProt ID: P61371; Molecular weight: 39kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) has been cited over 59 times in peer reviewed journals and is trusted by the scientific community.
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) has 6 independent reviews from customers.

Related Products
Antibody clone EP4182 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 594, Alexa Fluor® 555, Alexa Fluor® 568, Alexa Fluor® 488, Alexa Fluor® 647, APC, PE (ab203404, ab203405, ab203406, ab275990, ab277925, ab310815, ab310894).

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Islet 1 also known as ISL1 is a LIM-homeodomain transcription factor with a molecular mass of approximately 38 kDa. It plays an important role in regulating gene expression during the development of the nervous system and pancreas. You can find Islet 1 predominantly in motor neurons pancreatic beta cells and cardiac muscle cells. Its expression is important during embryonic stages for cell differentiation and maturation. Researchers use "Isl 1 neuronal marker" antibodies to identify this protein's presence in tissue.
Biological function summary

Islet 1 is involved in the differentiation and survival of various cell types. As part of a transcription complex it regulates downstream target genes essential for the development of motor neurons and cardiac muscle. It also contributes significantly to the developmental pathways of insulin-producing beta cells in the pancreas. The ISL1 marker is therefore an important tool for studying cell lineage and fate in developmental biology.

Pathways

Islet 1 is involved in key developmental signaling pathways including the Notch signaling and Wnt signaling pathways. In these pathways Islet 1 collaborates with other proteins like Lhx3 and Ngn2 to ensure proper gene activation for neuronal and cardiac development. Wnt signaling affects the expression of isl 1 marker genes contributing to precise spatial and temporal regulation during embryogenesis.

Islet 1 has links to congenital heart defects and type 2 diabetes. Mutations or altered expression of this protein can disrupt pancreatic beta cell function and motor neuron development. In congenital heart disease the interaction of Islet 1 with proteins like Nkx2.5 is critical. Its role in type 2 diabetes ties with the functioning of other proteins involved in insulin secretion making it a potential target for therapeutic intervention in these conditions.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1 (By similarity). Binds to insulin gene enhancer sequences (By similarity). Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart (By similarity).
See full target information ISL1

Publications (77)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40704991

2025

Rapid and inducible mislocalization of endogenous TDP43 in a novel human model of amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna Ganssauge,Sophie Hawkins,Seema Chandramohan Namboori,Szi Kay Leung,Jonathan Mill,Akshay Bhinge

Scientific reports 15:12516 PubMed40216954

2025

CaMK2A/CREB pathway activation is associated with enhanced mitophagy and neuronal apoptosis in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochun Yang,Yuxin Zhang,Yikun Zhou,Mingzhi Liu,Haiyan Zhao,Yang Yang,Jianyun Su

Current protocols 5:e70091 PubMed39781605

2025

Generation of Retinal Ganglion Cells from Reprogrammed Keratocytes of Non-Glaucoma and Glaucoma Donors.

Applications

Unspecified application

Species

Unspecified reactive species

Shahna S Hameed,Tasneem P Sharma

Scientific reports 14:31182 PubMed39732807

2024

iPSC-derived human sensory neurons reveal a subset of TRPV1 antagonists as anti-pruritic compounds.

Applications

Unspecified application

Species

Unspecified reactive species

Shermaine Huiping Tay,Jeremy Kah Sheng Pang,Winanto Ng,Chong Yi Ng,Zi Jian Khong,Zheng-Shan Chong,Boon Seng Soh,Shi-Yan Ng

Stem cell research & therapy 15:465 PubMed39639398

2024

Using magnetic resonance relaxometry to evaluate the safety and quality of induced pluripotent stem cell-derived spinal cord progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jerome Tan,Jiahui Chen,Daniel Roxby,Wai Hon Chooi,Tan Dai Nguyen,Shi Yan Ng,Jongyoon Han,Sing Yian Chew

Scientific reports 14:25166 PubMed39448766

2024

A primate model animal revealed the inter-species differences and similarities in the subtype specifications of the spiral ganglion neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Hosoya,Masafumi Ueno,Marie N Shimanuki,Takanori Nishiyama,Naoki Oishi,Hiroyuki Ozawa

Cell reports. Medicine 5:101659 PubMed39067446

2024

Isogenic patient-derived organoids reveal early neurodevelopmental defects in spinal muscular atrophy initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Grass,Zeynep Dokuzluoglu,Felix Buchner,Ines Rosignol,Joshua Thomas,Antonio Caldarelli,Anna Dalinskaya,Jutta Becker,Fabian Rost,Michele Marass,Brunhilde Wirth,Marc Beyer,Lorenzo Bonaguro,Natalia Rodriguez-Muela

Molecular therapy : the journal of the American Society of Gene Therapy 32:2584-2603 PubMed38845196

2024

In vivo selection in non-human primates identifies AAV capsids for on-target CSF delivery to spinal cord.

Applications

Unspecified application

Species

Unspecified reactive species

Killian S Hanlon,Ming Cheng,Roberto Montoro Ferrer,Jae Ryun Ryu,Boram Lee,Demitri De La Cruz,Nikita Patel,Paula Espinoza,Miguel C Santoscoy,Yi Gong,Carrie Ng,Diane M Nguyen,Josette Nammour,Sean W Clark,Vivi M Heine,Woong Sun,Karen Kozarsky,Casey A Maguire

Genome biology 25:140 PubMed38807229

2024

ePRINT: exonuclease assisted mapping of protein-RNA interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie Hawkins,Alexandre Mondaini,Seema C Namboori,Grady G Nguyen,Gene W Yeo,Asif Javed,Akshay Bhinge

Nature cardiovascular research 2:1060-1077 PubMed38524149

2024

Translational landscape of direct cardiac reprogramming reveals a role of Ybx1 in repressing cardiac fate acquisition.

Applications

Unspecified application

Species

Unspecified reactive species

Yifang Xie,Qiaozi Wang,Yuchen Yang,David Near,Haofei Wang,Marazzano Colon,Christopher Nguyen,Conor Slattery,Benjamin Keepers,Gregory Farber,Tzu-Wen Wang,Sung-Ho Lee,Yen-Yu Ian Shih,Jiandong Liu,Li Qian
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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