Name: Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker
SKU: ab178400
Rating: 5 (1 reviews)

Anti-Islet 1 antibody [EPR10362] ab178400 is a rabbit monoclonal antibody that is used in Islet 1 western blotting and IHC. Suitable for human samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity confirmed with ISL1 knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

Images

Immunoprecipitation - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (AB178400), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	Flow Cyt	WB	ICC/IF
Human	Tested	Tested	Not recommended	Tested	Not recommended
Mouse	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/6000	Notes For unpurified use at 1/250 - 1/500 Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes For unpurified use at 1/250 - 1/500 Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes For unpurified use at 1/250 - 1/500 Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10 - 1/100	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/10000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Associated Products

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Target data

See full target information ISL1

Function

DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1 (By similarity). Binds to insulin gene enhancer sequences (By similarity). Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart (By similarity).

Alternative names

Insulin gene enhancer protein ISL-1, Islet-1, ISL1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR10362
Purification technique: Affinity purification Protein A
Specificity: In western blot, we observe a specific band at ~40kDa which is not seen in KO cell lines. A cross-reactive band was observed in the wild-type and knockout cells. The additional band below this band of interest is seen at ~38kDa in both the WT and KO cells. We are unsure as to the identity of these extra bands.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Islet 1 also known as ISL1 is a LIM-homeodomain transcription factor with a molecular mass of approximately 38 kDa. It plays an important role in regulating gene expression during the development of the nervous system and pancreas. You can find Islet 1 predominantly in motor neurons pancreatic beta cells and cardiac muscle cells. Its expression is important during embryonic stages for cell differentiation and maturation. Researchers use "Isl 1 neuronal marker" antibodies to identify this protein's presence in tissue.

Biological function summary

Islet 1 is involved in the differentiation and survival of various cell types. As part of a transcription complex it regulates downstream target genes essential for the development of motor neurons and cardiac muscle. It also contributes significantly to the developmental pathways of insulin-producing beta cells in the pancreas. The ISL1 marker is therefore an important tool for studying cell lineage and fate in developmental biology.

Pathways

Islet 1 is involved in key developmental signaling pathways including the Notch signaling and Wnt signaling pathways. In these pathways Islet 1 collaborates with other proteins like Lhx3 and Ngn2 to ensure proper gene activation for neuronal and cardiac development. Wnt signaling affects the expression of isl 1 marker genes contributing to precise spatial and temporal regulation during embryogenesis.

Associated diseases and disorders

Islet 1 has links to congenital heart defects and type 2 diabetes. Mutations or altered expression of this protein can disrupt pancreatic beta cell function and motor neuron development. In congenital heart disease the interaction of Islet 1 with proteins like Nkx2.5 is critical. Its role in type 2 diabetes ties with the functioning of other proteins involved in insulin secretion making it a potential target for therapeutic intervention in these conditions.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

Immunoprecipitation - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400)
ab178400 (purified ) at 1/20 dilution (1ug) immunoprecipitating Islet 1 in SH-SY5Y whole cell lysate.
Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab178400 & SH-SY5Y whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab178400 in SH-SY5Y whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400)
Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400)
Islet 1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Islet 1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue sections labeling Islet 1 with purified ab178400 at 1/6000 dilution (0.03 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400)
Islet 1 Western blot staining using rabbit Anti-Islet 1 antibody
Lanes 1 - 4: Merged signal (red and green). Green - ab178400 observed at 40 kDa. Red - loading control, ab18058, observed at 130 kDa.
Unpurified ab178400 was shown to recognize Islet 1 in wild-type HAP1 cells as signal was lost at the expected MW in Islet 1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Islet 1 knockout samples were subjected to SDS-PAGE. ab178400 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1,000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: Islet 1 knockout HAP1 whole cell lysate at 20 µg
Lane 3: SH-SY5Y whole cell lysate at 20 µg
Lane 4: HepG2 whole cell lysate at 20 µg
Predicted band size: 39 kDa
Western blot - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400)
Islet 1 Western blot staining using rabbit Anti-Islet 1 antibody
All lanes: Western blot - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400) at 1/10000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Immunoprecipitation - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400)
Islet 1 was immunoprecipitated from 0.35 mg SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10μg with unpurifed ab178400 at 1:50 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using unpurified ab178400 1:1000 dilution (2 μg/ml). VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary antibody at 1:1000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10μg
Lane 2: ab178400 IP in SH-SY5Y whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab178400 in SH-SY5Y whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second
All lanes: Immunoprecipitation - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400)
Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400)
Islet 1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Islet 1 antibody
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Islet 1 with ab178400 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human pancreatic islet. The section was incubated with Anti-DLL3 antibody [EPR22592-18] ab229902 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Western blot - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400)
Islet 1 Western blot staining of A375 (HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates) whole cell lysates using rabbit Anti-Islet 1 antibody
All lanes: Western blot - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400) at 1/10000 dilution
All lanes: A375 (HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates) whole cell lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [EPR10362] (ab178400)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded, human gastric neuroendocrine carcinoma tissue labeling Islet 1 with unpurified ab178400 at a 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400)
Western blot analysis on Immunoprecipitation pellet from either Lane 1) SH-SY5Y cell lysate, or Lane 2) 1xPBS (negative control); showing Islet 1, using unpurifed ab178400 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400)
Developed using the ECL technique.
Predicted band size: 39 kDa
Western blot - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400)
Islet 1 Western blot staining using rabbit Anti-Islet 1 antibody
All lanes: Western blot - Anti-Islet 1 antibody [EPR10362] - Neural Stem Cell Marker (ab178400) at 1/1000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
Lane 2: K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate at 10 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg
Lane 4: SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate at 10 µg
Secondary
All lanes: Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [EPR10362] (ab178400)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded, human small cell lung carcinoma tissue labeling Islet 1 with unpurified ab178400 at a 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.