AB31533

Anti-Isoleucyl tRNA synthetase antibody

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(3 Publications)

Rabbit Polyclonal Isoleucyl tRNA synthetase antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IARS1.

View Alternative Names

IARS, IARS1, Isoleucyl-tRNA synthetase, IRS, IleRS

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Isoleucyl tRNA synthetase antibody (AB31533)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Isoleucyl tRNA synthetase antibody (AB31533)

ICC/IF image of ab31533 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31533, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-Isoleucyl tRNA synthetase antibody (AB31533)

All lanes:

Western blot - Anti-Isoleucyl tRNA synthetase antibody (ab31533) at 1/2000 dilution

All lanes:

whole cell lysate prepared from HEK293 cells at 50 µg/mL

Secondary

All lanes:

Goat anti-rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 145 kDa

Observed band size: 150 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IARS1.

P41252

Specificity

This antibody reacts specifically with Isoleucyl tRNA synthetase protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.05% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Isoleucyl tRNA synthetase also known as IARS or IleRS is an essential enzyme that charges tRNA molecules with isoleucine. This enzyme weighs approximately 126 kDa and is part of the aminoacyl-tRNA synthetase family. By catalyzing the attachment of isoleucine to its corresponding tRNA it ensures the correct translation of mRNA into proteins. IARS is ubiquitously expressed in the cytoplasm of various cell types and tissues playing a critical role in protein synthesis.

Biological function summary

IARS plays an important role in maintaining cellular homeostasis by ensuring the fidelity of protein translation. It forms part of a larger complex of aminoacyl-tRNA synthetases known for its multi-enzyme complex formation which assists in proper protein synthesis. Through its function in aminoacylation IARS maintains the balance necessary for cellular activity and division reflecting its importance across multiple biological processes.

Pathways

IARS has important responsibilities in the translation process which is a fundamental pathway in gene expression. In the context of translation the enzyme contributes to the aminoacyl-tRNA biosynthesis pathway often interacting with other tRNA synthetases to stabilize protein synthesis. Additionally IARS can relate to the mTOR signaling pathway indicating its connection with nutrient-sensing mechanisms and its role in regulating cell growth and proliferation.

Altered IARS function has connections to various neurological disorders such as CARS-related Perrault Syndrome where it may interact with cysteinyl-tRNA synthetase (CARS). Additionally mutations in IARS can relate to liver disorders like Vanishing White Matter (VWM) disease where it is associated with endoplasmic reticulum stress pathways and connected proteins such as eIF2B. Understanding these interactions helps in designing therapeutic strategies targeting the protein for treatment interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction : the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.

See full target information IARS1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Viruses 15: PubMed36851687

2023

HIV-1 Gag Binds the Multi-Aminoacyl-tRNA Synthetase Complex via the EPRS Subunit.

Applications

Unspecified application

Species

Unspecified reactive species

Danni Jin,Yiping Zhu,Heidi L Schubert,Stephen P Goff,Karin Musier-Forsyth

The Journal of biological chemistry 298:101757 PubMed35202654

2022

Tissue-specific alternative splicing separates the catalytic and cell signaling functions of human leucyl-tRNA synthetase.

Applications

Unspecified application

Species

Unspecified reactive species

Max Baymiller,Benjamin Nordick,Connor M Forsyth,Susan A Martinis

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33568529

2021

Local translation in nuclear condensate amyloid bodies.

Applications

Unspecified application

Species

Unspecified reactive species

Phaedra R Theodoridis,Michael Bokros,Dane Marijan,Nathan C Balukoff,Dazhi Wang,Chloe C Kirk,Taylor D Budine,Harris D Goldsmith,Miling Wang,Timothy E Audas,Stephen Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Isoleucyl tRNA synthetase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Isoleucyl tRNA synthetase antibody (AB31533)

Western blot - Anti-Isoleucyl tRNA synthetase antibody (AB31533)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise