AB151557

Anti-Isoleucyl tRNA synthetase antibody [EPR10266(B)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Isoleucyl tRNA synthetase antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

IARS, IARS1, Isoleucyl-tRNA synthetase, IRS, IleRS

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Isoleucyl tRNA synthetase antibody [EPR10266(B)] (AB151557)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Isoleucyl tRNA synthetase antibody [EPR10266(B)] (AB151557)

Immunofluorescent analysis of HeLa cells labeling Isoleucyl tRNA synthetase with ab151557 at 1/1000 dilution.

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Western blot - Anti-Isoleucyl tRNA synthetase antibody [EPR10266(B)] (AB151557)

All lanes:

Western blot - Anti-Isoleucyl tRNA synthetase antibody [EPR10266(B)] (ab151557)

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Predicted band size: 145 kDa

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Carrier free

Anti-Isoleucyl tRNA synthetase antibody [EPR10266(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10266(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Isoleucyl tRNA synthetase also known as IARS or IleRS is an essential enzyme that charges tRNA molecules with isoleucine. This enzyme weighs approximately 126 kDa and is part of the aminoacyl-tRNA synthetase family. By catalyzing the attachment of isoleucine to its corresponding tRNA it ensures the correct translation of mRNA into proteins. IARS is ubiquitously expressed in the cytoplasm of various cell types and tissues playing a critical role in protein synthesis.

Biological function summary

IARS plays an important role in maintaining cellular homeostasis by ensuring the fidelity of protein translation. It forms part of a larger complex of aminoacyl-tRNA synthetases known for its multi-enzyme complex formation which assists in proper protein synthesis. Through its function in aminoacylation IARS maintains the balance necessary for cellular activity and division reflecting its importance across multiple biological processes.

Pathways

IARS has important responsibilities in the translation process which is a fundamental pathway in gene expression. In the context of translation the enzyme contributes to the aminoacyl-tRNA biosynthesis pathway often interacting with other tRNA synthetases to stabilize protein synthesis. Additionally IARS can relate to the mTOR signaling pathway indicating its connection with nutrient-sensing mechanisms and its role in regulating cell growth and proliferation.

Altered IARS function has connections to various neurological disorders such as CARS-related Perrault Syndrome where it may interact with cysteinyl-tRNA synthetase (CARS). Additionally mutations in IARS can relate to liver disorders like Vanishing White Matter (VWM) disease where it is associated with endoplasmic reticulum stress pathways and connected proteins such as eIF2B. Understanding these interactions helps in designing therapeutic strategies targeting the protein for treatment interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction : the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.

See full target information IARS1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 11:2364-2380 PubMed33500730

2021

Endocytic pathway inhibition attenuates extracellular vesicle-induced reduction of chemosensitivity to bortezomib in multiple myeloma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chenggong Tu,Zhimin Du,Hui Zhang,Yueyuan Feng,Yujun Qi,Yongjiang Zheng,Jinbao Liu,Jinheng Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Isoleucyl tRNA synthetase antibody [EPR10266(B)]

Immunocytochemistry/ Immunofluorescence - Anti-Isoleucyl tRNA synthetase antibody [EPR10266(B)] (AB151557)

Western blot - Anti-Isoleucyl tRNA synthetase antibody [EPR10266(B)] (AB151557)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise