AB422

Anti-Isopeptide antibody [81D1C2]

Name: Anti-Isopeptide antibody [81D1C2] (ab422) | Abcam
Brand: Abcam Limited
SKU: AB422
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(11 Publications)

Mouse Monoclonal Isopeptide antibody. Suitable for WB and reacts with Chemical samples. Cited in 11 publications.

View Alternative Names

Isopeptide bond

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

81D1C2

Isotype

IgG1

Light chain type

kappa

Carrier free

Applications

applications

Specificity

This antibody reacts with the isopeptide : epsilon-(gamma-glutamyl) lysine. There is no cross reaction with either lysine or glutamine. They are specific to the epsilon-(gamma-glutamyl) lysine bond formed by transglutaminase activity.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/50 - 1/500", "WB-species-notes": "<p></p>" } } }

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Product details

The ability of this antibody to fix complement is unknown. The isopeptide may be purchased from Bachem Catalog number G-1970.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Isopeptide plays an important role mechanically in the formation of stable covalent bonds between proteins. It often refers to the linkage created by the isopeptide bond a type of amide bond that connects the side chain of an amino acid to another chain. These bonds are also significant in protein crosslinking processes. Isopeptide bonds are found in various structural proteins and ubiquitin-like proteins. The target is normally expressed in tissues with high protein turnover and structural maintenance functions.

Biological function summary

The functions of isopeptide bonds are central to maintaining protein structure and function. Isopeptide bonds are key in multimeric protein complexes such as those involving ubiquitin and SUMO. These complexes help in post-translational modification processes that regulate protein degradation DNA repair and signal transduction. This ability to form stable linker bonds influences cell cycle and immune responses.

Pathways

These bonds partake in pathways related to protein homeostasis and cell signaling. Isopeptide bonds are essential in the ubiquitin-proteasome pathway facilitating the tagging of proteins for degradation. Related proteins such as ubiquitin and E3 ligases are involved in these pathways. Isopeptide bonds also participate in the signal transduction pathways by modifying signaling proteins through ubiquitination.

Isopeptide bonds have been linked to neurodegenerative diseases and certain types of cancers. Aberrations in the ubiquitin-proteasome pathway can lead to the accumulation of misfolded proteins contributing to conditions like Parkinson's disease. Proteins such as ubiquitin and E3 ligases have connections in these disorders. Moreover dysregulation of protein complex formation via isopeptide bonds can result in tumorigenesis due to disrupted cell cycle control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Isopeptide

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 34:299-316.e6 PubMed35108516

2022

Erythrocyte transglutaminase-2 combats hypoxia and chronic kidney disease by promoting oxygen delivery and carnitine homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Xu,Changhan Chen,Yujin Zhang,Monika Dzieciatkowska,Benjamin C Brown,Weiru Zhang,Tingting Xie,Osheiza Abdulmalik,Anren Song,Chao Tong,Hongbo Qi,Robert Roach,Rodney E Kellems,Angelo D'Alessandro,Yang Xia

The Journal of biological chemistry 293:13566-13577 PubMed30006344

2018

Chlorpyrifos oxon promotes tubulin aggregation via isopeptide cross-linking between diethoxyphospho-Lys and Glu or Asp: Implications for neurotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Lawrence M Schopfer,Oksana Lockridge

Human molecular genetics 23:3421-31 PubMed24608227

2014

STAG3 is a strong candidate gene for male infertility.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Llano,Laura Gomez-H,Ignacio García-Tuñón,Manuel Sánchez-Martín,Sandrine Caburet,Jose Luis Barbero,John C Schimenti,Reiner A Veitia,Alberto M Pendas

Hypertension (Dallas, Tex. : 1979) 63:353-61 PubMed24191290

2013

Tissue transglutaminase contributes to the pathogenesis of preeclampsia and stabilizes placental angiotensin receptor type 1 by ubiquitination-preventing isopeptide modification.

Applications

Species

Unspecified reactive species

Chen Liu,Wei Wang,Nicholas Parchim,Roxanna A Irani,Sean C Blackwell,Baha Sibai,Jianping Jin,Rodney E Kellems,Yang Xia

Malaria journal 10:14 PubMed21255444

2011

Plasmodium falciparum liver stage antigen-1 is cross-linked by tissue transglutaminase.

Applications

Unspecified application

Species

Unspecified reactive species

William S Nicoll,John B Sacci,Carlo Rodolfo,Giuseppina Di Giacomo,Mauro Piacentini,Zoe Jm Holland,Christian Doerig,Michael R Hollingdale,David E Lanar

Experimental and molecular pathology 88:15-25 PubMed19931242

2009

The interaction of angiocidin with tissue transglutaminase.

Applications

Unspecified application

Species

Unspecified reactive species

Darryl Z L'Heureux,Vicki L Rothman,George P Tuszynski

Molecular cancer 4:33 PubMed16153302

2005

Tissue transglutaminase-induced alterations in extracellular matrix inhibit tumor invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Lingegowda S Mangala,Banu Arun,Aysegul A Sahin,Kapil Mehta

International journal of cancer 118:310-6 PubMed16052522

2005

SPARC expression is associated with impaired tumor growth, inhibited angiogenesis and changes in the extracellular matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandre Chlenski,Shuqing Liu,Lisa J Guerrero,Qiwei Yang,Yufeng Tian,Helen R Salwen,Peter Zage,Susan L Cohn

Journal of neuroscience methods 134:151-8 PubMed15003381

2004

Immunoblot analysis reveals that isopeptide antibodies do not specifically recognize the epsilon-(gamma-glutamyl)lysine bonds formed by transglutaminase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Gail V W Johnson,Reginald LeShoure

International journal of cancer 48:221-6 PubMed1673452

1991

Transglutaminase activity and N epsilon (gamma glutamyl) lysine isopeptide levels during cell growth: an enzymic and immunological study.

Applications

Unspecified application

Species

Unspecified reactive species

S el Alaoui,S Legastelois,A M Roch,J Chantepie,G Quash

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Isopeptide antibody [81D1C2]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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Product promise