Isotype controls

Achieve flawless experimental setups with isotype controls engineered for minimal cross-reactivity and maximum reliability. These validated controls are part of our portfolio of over 60k primary antibodies, which are supported by over 600k citations. They will save you time and hassle in the lab by providing the definitive baseline your research demands.

Browse all isotype controls

button-secondary

What is an isotype control antibody?

Isotype controls isotype control antibodies are antibodies that do not react with a specific antigen and are used as negative controls to correctly evaluate the results obtained from flow cytometry. immunohistochemistry and other applications.

When should I use an isotype control?

Isotype controls are essential in antibody-based experiments such as immunohistochemistry and flow cytometry to check for non-specific binding—such as interactions with unrelated proteins or Fc receptors on cell surfaces. By using an isotype control antibody that matches the isotype of your primary antibody but lacks target specificity, you can distinguish true signal from background noise and confidently assess the specificity and strength of your primary antibody’s binding.

How do I choose the right isotype control?

Isotype control antibodies should be matched to the primary antibody against your target:

Host species and clonality
Class and subclass
Conjugation form

For example, if the primary antibody is a conjugated mouse monoclonal IgG1 antibody, then the isotype control should also be a conjugated mouse monoclonal IgG1antibody.

The concentration and reaction conditions for the isotype control should be the same as those for the primary antibody.