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AB222793

Anti-ISPD antibody

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(1 Publication)

Rabbit Polyclonal ISPD antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CRPPA aa 1-300.

View Alternative Names

ISPD, CRPPA, D-ribitol-5-phosphate cytidylyltransferase, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein, Isoprenoid synthase domain-containing protein, hISPD

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISPD antibody (AB222793)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISPD antibody (AB222793)

Paraffin embedded human liver cancer tissue stained for ISPD with ab222793 at a 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISPD antibody (AB222793)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISPD antibody (AB222793)

Paraffin embedded human testis tissue stained for ISPD with ab222793 at a 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-ISPD antibody (AB222793)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ISPD antibody (AB222793)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ISPD (green) using ab222793 at a 1/100 dilution in ICC/IF. Secondary : Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CRPPA aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

A4D126

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ISPD also known as isoprenoid synthase domain-containing protein functions as an important enzyme in the cellular biosynthesis process. It has a molecular mass of approximately 46 kDa. This protein plays a central role in the formation of dolichol phosphate sugars which are essential for the glycosylation of proteins. The ISPD gene expresses highly in tissues such as muscle and heart where glycosylation processes are vital for normal function.
Biological function summary

ISPD contributes to the synthesis of critical isoprenoid compounds. ISPD does not operate alone it functions as part of a larger machinery that ensures proper protein modification which allows correct cellular location and function of glycoproteins. In this setting ISPD is important because it influences how cells manage structural stability and communication between cellular environments.

Pathways

ISPD participates significantly in the dolichol phosphate biosynthesis pathway. This pathway is important for enabling protein glycosylation in the endoplasmic reticulum. ISPD interacts with DPM1 an important protein in the dolichol cycle to effectively transport glycan precursors across the membrane. ISPD also impacts the protein N-linked glycosylation pathway where it assists in modifying proteins for functional diversity and stability.

ISPD has a strong connection to a group of muscular dystrophies notably Walker-Warburg Syndrome. This syndrome is characterized by severe muscular and ocular abnormalities due to defects in O-mannosyl glycan synthesis. Mutations in the ISPD gene can impair normal function leading to these pathologies. Furthermore its interactions with proteins like POMT1 emphasize its pivotal role in muscle integrity as disruptions in these associations can contribute to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytidylyltransferase required for protein O-linked mannosylation (PubMed : 22522420, PubMed : 22522421, PubMed : 26687144, PubMed : 26923585, PubMed : 27130732, PubMed : 27601598). Catalyzes the formation of CDP-ribitol nucleotide sugar from D-ribitol 5-phosphate (PubMed : 26687144, PubMed : 26923585, PubMed : 27130732). CDP-ribitol is a substrate of FKTN during the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed : 26687144, PubMed : 26923585, PubMed : 27130732). Shows activity toward other pentose phosphate sugars and mediates formation of CDP-ribulose or CDP-ribose using CTP and ribulose-5-phosphate or ribose-5-phosphate, respectively (PubMed : 26687144). Not Involved in dolichol production (PubMed : 26687144).
See full target information CRPPA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Regenerative biomaterials 12:rbaf092 PubMed41048975

2025

L-arginine-loaded microneedle patch enhances diabetic wound healing by regulating macrophage polarisation and mitochondrial homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Wang,Shun Yao,Qingyun Mo,Mingyue Chen,Danfeng He,Lingfeng Yan,Chang Wang,Tao Zou,Gaoxing Luo,Jun Deng
View all publications
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