Rabbit Polyclonal IST1 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IST1 aa 1-100.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human IST1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P53990
Reactivity data
Select an application| IHC-P | ICC | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Orangutan, Cow | Dilution info - | Notes - |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Fixation/Permeabilization: PFA/Triton X-100 |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Orangutan, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Orangutan, Cow | Dilution info - | Notes - |
Target data
Function
ESCRT-III-like protein involved in cytokinesis, nuclear envelope reassembly and endosomal tubulation (PubMed:19129479, PubMed:26040712, PubMed:28242692). Is required for efficient abscission during cytokinesis (PubMed:19129479). Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells (PubMed:19129479, PubMed:19129480). During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing (PubMed:26040712). Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2 (PubMed:28242692). Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST (PubMed:23897888).
Alternative names
KIAA0174, IST1, IST1 homolog, hIST1, Charged multivesicular body protein 8, Putative MAPK-activating protein PM28, CHMP8
Recommended products
Rabbit Polyclonal IST1 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IST1 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human IST1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P53990
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IST1 also known as Increased Sodium Tolerance 1 is a protein involved in membrane trafficking within cells. It weighs around 37 kDa and expresses mainly in the cytoplasm of various cell types especially in tissues with high endocytic and exocytic activity. Researchers often study IST1 to understand its role in the endosomal sorting process. In addition to its presence in human tissues IST1 shows conservation across different species highlighting its importance in cellular functions.
- Biological function summary
The IST1 protein operates as a regulator within the ESCRT (endosomal sorting complexes required for transport) machinery. This complex plays a role in the sorting of ubiquitinated cargoes into multivesicular bodies influencing their fate in lysosomal degradation. IST1 interacts with other proteins in the ESCRT-III complex facilitating the proper scission of membrane necks during vesicle budding processes. Its function finds relevance in maintaining cellular homeostasis by orchestrating the turnover of membrane proteins and lipids.
- Pathways
IST1 participates in the ESCRT pathway which is integral for endosomal transportation and membrane reformation processes such as cytokinesis and autophagy. In this context IST1 interacts with proteins such as CHMP1A and CHMP1B serving a critical role in the endosomal sorting and degradation pathway. Proper functioning of IST1 is necessary for the correct execution of membrane repair processes aiding in cellular recycling mechanisms.
- Associated diseases and disorders
IST1 exhibits links to neurodegenerative disorders and viral infections. Its role in membrane trafficking connects it to Alzheimer's disease through interactions with proteins like APP and PSEN1 which are related to amyloid-beta plaque formation. Furthermore viruses exploit the ESCRT machinery including IST1 for viral budding and release influencing the progression of viral diseases. Researchers continue to explore IST1's potential as a therapeutic target in such conditions given its involvement in these critical cellular mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunocytochemistry - Anti-IST1 antibody (ab244502)
PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for IST1 (green) using ab244502 at 4 μg/ml in ICC.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IST1 antibody (ab244502)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling IST1 with ab244502 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-IST1 antibody (ab244502)
All lanes: Western blot - Anti-IST1 antibody (ab244502) at 0.4 µg/mL
Lane 1: NTERA-2 (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 2: SK-BR-3 (human mammary gland adenocarcinoma cell line) whole cell lysate
Predicted band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IST1 antibody (ab244502)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling IST1 with ab244502 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IST1 antibody (ab244502)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human duodenum tissue labelling IST1 with ab244502 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IST1 antibody (ab244502)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling IST1 with ab244502 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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