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AB243732

Anti-ITFG2 antibody

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Rabbit Polyclonal ITFG2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KICSTOR complex protein ITFG2 aa 200-350.

View Alternative Names

KICSTOR complex protein ITFG2, Integrin-alpha FG-GAP repeat-containing protein 2, ITFG2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ITFG2 antibody (AB243732)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ITFG2 antibody (AB243732)

PFA fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells labeling ITFG2 using ab243732 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITFG2 antibody (AB243732)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITFG2 antibody (AB243732)

Formalin-fixed, paraffin-embedded human colon tissue stained for ITFG2 with ab243732 at a 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-ITFG2 antibody (AB243732)
  • WB

Supplier Data

Western blot - Anti-ITFG2 antibody (AB243732)

All lanes:

Western blot - Anti-ITFG2 antibody (ab243732) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

ITFG2 overexpression HEK-293T cell lysate

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KICSTOR complex protein ITFG2 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

Q969R8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein ITFG2 or Integrin Alpha FG-GAP Repeat Containing 2 plays an essential role in cell adhesion and migration mechanisms. Its molecular mass is approximately 42 kDa. Researchers have identified ITFG2 expression in various tissues including the heart brain liver and skeletal muscle indicating its potential involvement in diverse physiological processes.
Biological function summary

ITFG2 contributes to cell-cell interaction and signaling. It acts as part of the integrin protein family which facilitates communication between cells and their surrounding environment. The protein participates in processes involving cell adhesion through forming complexes with other integrins influencing cellular responses. ITFG2 by interacting with other cellular components supports the maintenance of cell structure and tissue architecture.

Pathways

ITFG2's function integrates into significant biological pathways notably the integrin-mediated signaling pathway and focal adhesion pathway. These pathways involve cooperation with other proteins like integrin beta subunits which assist in transmitting signals that affect cell proliferation survival and differentiation. ITFG2's contribution to these pathways highlights its involvement in essential cellular events and homeostasis.

ITFG2 is linked to certain pathological conditions due to its role in cellular communication. Abnormal expression of ITFG2 has been associated with cancer progression as it may alter cell adhesion properties promoting metastasis. Additionally researchers are investigating its connection to cardiovascular diseases given its expression in heart tissue and interaction with integrin-related proteins in these contexts. Understanding ITFG2's function may therefore provide insights into therapeutic strategies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose.
See full target information KICSTOR complex protein ITFG2
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