AB195959

Anti-ITGA7 antibody [3C12]

Name: Anti-ITGA7 antibody [3C12] (ab195959) | Abcam
Brand: Abcam Limited
SKU: AB195959
Price: 495 USD
Availability: InStock

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(2 Publications)

Anti-ITGA7 antibody [3C12] (ab195959) is a mouse monoclonal antibody detecting ITGA7 in Flow Cytometry, IHC-P. Suitable for Human, Mouse.

View Alternative Names

UNQ406/PRO768, ITGA7, Integrin alpha-7

2 Images

Flow Cytometry - Anti-ITGA7 antibody [3C12] (AB195959)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-ITGA7 antibody [3C12] (AB195959)

Flow Cytometrical analysis of C2C12 cells labeling ITGA7 with ab195959 at 20 μg or an isotypic control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGA7 antibody [3C12] (AB195959)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGA7 antibody [3C12] (AB195959)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain, cortex tissue labeling ITGA7 with ab195959 at 10 μg/mL.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3C12

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, Flow Cyt

applications

Immunogen

Cell preparation containing Integrin alpha-7 protein. The exact immunogen used to generate this antibody is proprietary information.

Q61738

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10-20 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

What is this antibody validated in?
Anti-ITGA7 antibody [3C12] (ab195959) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human, Mouse samples.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ITGA7 also known as integrin alpha 7 is a transmembrane protein that plays an important role in cell adhesion and signaling. Weighing approximately 124 kDa ITGA7 is part of the integrin family of proteins functioning as a receptor that binds primarily to extracellular matrix proteins such as laminins. It is expressed largely in skeletal and cardiac muscle tissues but research shows its presence in other tissues like the nervous system.

Biological function summary

ITGA7 interacts with the beta-1 integrin subunit to form a heterodimeric receptor. This combination mediates cell-matrix adhesion promoting cell migration differentiation and survival. ITGA7 is also involved in the stabilization of mature muscle cells and development of muscle fibers. By engaging with specific laminin isoforms ITGA7 influences tissue architecture and function.

Pathways

Researchers recognize that ITGA7 is important in signaling pathways like the PI3K/Akt pathway which affects cell growth and survival. ITGA7 interacts with proteins like focal adhesion kinase (FAK) to transmit signals from the extracellular matrix to the inside of the cell. This communication helps regulate cellular responses and adaptability in dynamic environments.

ITGA7 has connections to muscular dystrophy and certain types of cancer. Mutations or altered expression of ITGA7 can lead to muscular dystrophy where muscle function becomes compromised. In cancer altered ITGA7 expression may affect tumor progression and metastasis with connections to proteins such as dystroglycan. Understanding these relationships is essential for developing targeted therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). Acts as a Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation (By similarity). Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells.

See full target information ITGA7

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancers 12: PubMed32033262

2020

Integrin α7 and Extracellular Matrix Laminin 211 Interaction Promotes Proliferation of Acute Myeloid Leukemia Cells and Is Associated with Granulocytic Sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nobuhiko Kobayashi,Tsukasa Oda,Makiko Takizawa,Takuma Ishizaki,Norifumi Tsukamoto,Akihiko Yokohama,Hisashi Takei,Takayuki Saitoh,Hiroaki Shimizu,Kazuki Honma,Kei Kimura-Masuda,Yuko Kuroda,Rei Ishihara,Yuki Murakami,Hirokazu Murakami,Hiroshi Handa

Oncotarget 9:18747-18759 PubMed29721158

2018

Acquired resistance to temsirolimus is associated with integrin α7 driven chemotactic activity of renal cell carcinoma .

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Engl,Jochen Rutz,Sebastian Maxeiner,Sorel Fanguen,Eva Juengel,Sebastian Koschade,Frederik Roos,Wael Khoder,Igor Tsaur,Roman A Blaheta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ITGA7 antibody [3C12]

Flow Cytometry - Anti-ITGA7 antibody [3C12] (AB195959)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGA7 antibody [3C12] (AB195959)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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